Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA
This study aimed to validate an analytical method to determine DNA concentration using standard reference material (NIST SRM 2372) and Sprague Dawley rat and human DNA. Microvolumes were used to analyse DNA samples. Linearity showed correlation coefficients higher than R ≥ 0.9950, and the precision...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2020/8896738 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832554065392828416 |
|---|---|
| author | Alejandro Monserrat García-Alegría Iván Anduro-Corona Cinthia Jhovanna Pérez-Martínez María Alba Guadalupe Corella-Madueño María Lucila Rascón-Durán Humberto Astiazaran-Garcia |
| author_facet | Alejandro Monserrat García-Alegría Iván Anduro-Corona Cinthia Jhovanna Pérez-Martínez María Alba Guadalupe Corella-Madueño María Lucila Rascón-Durán Humberto Astiazaran-Garcia |
| author_sort | Alejandro Monserrat García-Alegría |
| collection | DOAJ |
| description | This study aimed to validate an analytical method to determine DNA concentration using standard reference material (NIST SRM 2372) and Sprague Dawley rat and human DNA. Microvolumes were used to analyse DNA samples. Linearity showed correlation coefficients higher than R ≥ 0.9950, and the precision value was ≤2% CV. Trueness based on bias and the percentage of recovery showed bias values lower than Z-test with a 95% confidence level and a recovery percentage within the range (% Rec = 100% ± 5%), and the stability of the samples was 60 days (2–4°C). |
| format | Article |
| id | doaj-art-1a2925db60774668b577e0f5b55d5eff |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-1a2925db60774668b577e0f5b55d5eff2025-02-03T05:52:26ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792020-01-01202010.1155/2020/88967388896738Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNAAlejandro Monserrat García-Alegría0Iván Anduro-Corona1Cinthia Jhovanna Pérez-Martínez2María Alba Guadalupe Corella-Madueño3María Lucila Rascón-Durán4Humberto Astiazaran-Garcia5Universidad de Sonora, Departamento de Ciencias Químico Biológicas, Hermosillo, Sonora CP 83000, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C. (CIAD AC), Coordinación de Nutrición, Hermosillo, Sonora CP 83304, MexicoUniversidad de Sonora, Departamento de Ciencias Químico Biológicas, Hermosillo, Sonora CP 83000, MexicoUniversidad de Sonora, Departamento de Ciencias Químico Biológicas, Hermosillo, Sonora CP 83000, MexicoUniversidad de Sonora, Departamento de Ciencias Químico Biológicas, Hermosillo, Sonora CP 83000, MexicoUniversidad de Sonora, Departamento de Ciencias Químico Biológicas, Hermosillo, Sonora CP 83000, MexicoThis study aimed to validate an analytical method to determine DNA concentration using standard reference material (NIST SRM 2372) and Sprague Dawley rat and human DNA. Microvolumes were used to analyse DNA samples. Linearity showed correlation coefficients higher than R ≥ 0.9950, and the precision value was ≤2% CV. Trueness based on bias and the percentage of recovery showed bias values lower than Z-test with a 95% confidence level and a recovery percentage within the range (% Rec = 100% ± 5%), and the stability of the samples was 60 days (2–4°C).http://dx.doi.org/10.1155/2020/8896738 |
| spellingShingle | Alejandro Monserrat García-Alegría Iván Anduro-Corona Cinthia Jhovanna Pérez-Martínez María Alba Guadalupe Corella-Madueño María Lucila Rascón-Durán Humberto Astiazaran-Garcia Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA International Journal of Analytical Chemistry |
| title | Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA |
| title_full | Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA |
| title_fullStr | Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA |
| title_full_unstemmed | Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA |
| title_short | Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA |
| title_sort | quantification of dna through the nanodrop spectrophotometer methodological validation using standard reference material and sprague dawley rat and human dna |
| url | http://dx.doi.org/10.1155/2020/8896738 |
| work_keys_str_mv | AT alejandromonserratgarciaalegria quantificationofdnathroughthenanodropspectrophotometermethodologicalvalidationusingstandardreferencematerialandspraguedawleyratandhumandna AT ivanandurocorona quantificationofdnathroughthenanodropspectrophotometermethodologicalvalidationusingstandardreferencematerialandspraguedawleyratandhumandna AT cinthiajhovannaperezmartinez quantificationofdnathroughthenanodropspectrophotometermethodologicalvalidationusingstandardreferencematerialandspraguedawleyratandhumandna AT mariaalbaguadalupecorellamadueno quantificationofdnathroughthenanodropspectrophotometermethodologicalvalidationusingstandardreferencematerialandspraguedawleyratandhumandna AT marialucilarasconduran quantificationofdnathroughthenanodropspectrophotometermethodologicalvalidationusingstandardreferencematerialandspraguedawleyratandhumandna AT humbertoastiazarangarcia quantificationofdnathroughthenanodropspectrophotometermethodologicalvalidationusingstandardreferencematerialandspraguedawleyratandhumandna |