Influence of different priming treatments on germination potential and seedling establishment of four important hemp (Cannabis sativa L.) cultivars
Abstract Medicinal hemp (Cannabis sativa L.) is gaining popularity due to its natural products with potential therapeutic properties, and its cultivation has recently been initiated in developing countries such as Turkey, Iran, India, and Pakistan. The crop is primarily grown using feminized seeds o...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-025-86469-y |
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| author | Saba Latif Rahmatullah Qureshi Abdul Rauf NoshinIlyas Qaiser Hussain Syed Sabir Hussain Shah Sabith Rehman Arshad Mahmood Khan Nasrullah Khan Mostafa A. Abdel-Maksoud Abdul Malik Sabiha Fatima Bushra Hafeez Kiani |
| author_facet | Saba Latif Rahmatullah Qureshi Abdul Rauf NoshinIlyas Qaiser Hussain Syed Sabir Hussain Shah Sabith Rehman Arshad Mahmood Khan Nasrullah Khan Mostafa A. Abdel-Maksoud Abdul Malik Sabiha Fatima Bushra Hafeez Kiani |
| author_sort | Saba Latif |
| collection | DOAJ |
| description | Abstract Medicinal hemp (Cannabis sativa L.) is gaining popularity due to its natural products with potential therapeutic properties, and its cultivation has recently been initiated in developing countries such as Turkey, Iran, India, and Pakistan. The crop is primarily grown using feminized seeds or popular varieties to achieve higher cannabidiol (CBD) yields, with seeds costing approximately $5–10 each. However, limited knowledge exists on hemp seed germination under different seed priming techniques and variations in seedling growth parameters such as root and shoot lengths, as well as the influence of in vitro germination and growth in soil and cocopeat media. This study hypothesizes that various seed priming methods could significantly enhance hemp seed germination and different growth media may distinctly impact seedling growth of the tested cultivars. To test this, the study evaluated four cultivars/chemovars (viz., Fedora-17, Finola, CBD Pink Kush, and a local variety, Gulistan) under five seed priming treatments: indole butyric acid (IBA), moringa leaf extract, KNO3, hydro-priming, and a control (no priming). A randomized complete block design (RCBD) was used, and experimental data (in triplicate) were analyzed through analysis of variance (ANOVA) using a time-to-event approach. The findings revealed that IBA significantly (p < 0.05) improved seed germination rates, with CBD Pink Kush achieving the highest germination response (83%) among the tested cultivars. Furthermore, cocopeat demonstrated superior performance as a seedling growth medium across all cultivars, indicating its potential for hemp cultivation practices. This pioneering study provides a baseline for understanding hemp seed germination and seedling growth under different priming and growth media conditions, offering valuable insights for future research aimed at industrial and medicinal hemp production. |
| format | Article |
| id | doaj-art-1a21de1648ed428f83b387e40b6022ee |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-1a21de1648ed428f83b387e40b6022ee2025-01-26T12:34:29ZengNature PortfolioScientific Reports2045-23222025-01-0115111510.1038/s41598-025-86469-yInfluence of different priming treatments on germination potential and seedling establishment of four important hemp (Cannabis sativa L.) cultivarsSaba Latif0Rahmatullah Qureshi1Abdul Rauf2NoshinIlyas3Qaiser Hussain4Syed Sabir Hussain Shah5Sabith Rehman6Arshad Mahmood Khan7Nasrullah Khan8Mostafa A. Abdel-Maksoud9Abdul Malik10Sabiha Fatima11Bushra Hafeez Kiani12Department of Botany, Pir Mehr Ali Shah Arid Agriculture University RawalpindiDepartment of Botany, Pir Mehr Ali Shah Arid Agriculture University RawalpindiDepartment of Zoology, Faculty of Science, University of Azad Jammu and KashmirDepartment of Botany, Pir Mehr Ali Shah Arid Agriculture University RawalpindiDepartment of Environmental and Soil Sciences, Pir Mehr Ali Shah Arid Agriculture University RawalpindiDepartment of Botany, Pir Mehr Ali Shah Arid Agriculture University RawalpindiDepartment of Botany, Hazara University MansehraDepartment of Botany, Government Hashmat Ali Islamia Associate College RawalpindiDepartment of Botany, University of MalakandBotany and Microbiology department, College of Science, King Saud UniversityDepartment of Pharmaceutics, College of Pharmacy, King Saud UniversityDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud Universityartment of Biology and Biotechnology, Worcester Polytechnic InstituteAbstract Medicinal hemp (Cannabis sativa L.) is gaining popularity due to its natural products with potential therapeutic properties, and its cultivation has recently been initiated in developing countries such as Turkey, Iran, India, and Pakistan. The crop is primarily grown using feminized seeds or popular varieties to achieve higher cannabidiol (CBD) yields, with seeds costing approximately $5–10 each. However, limited knowledge exists on hemp seed germination under different seed priming techniques and variations in seedling growth parameters such as root and shoot lengths, as well as the influence of in vitro germination and growth in soil and cocopeat media. This study hypothesizes that various seed priming methods could significantly enhance hemp seed germination and different growth media may distinctly impact seedling growth of the tested cultivars. To test this, the study evaluated four cultivars/chemovars (viz., Fedora-17, Finola, CBD Pink Kush, and a local variety, Gulistan) under five seed priming treatments: indole butyric acid (IBA), moringa leaf extract, KNO3, hydro-priming, and a control (no priming). A randomized complete block design (RCBD) was used, and experimental data (in triplicate) were analyzed through analysis of variance (ANOVA) using a time-to-event approach. The findings revealed that IBA significantly (p < 0.05) improved seed germination rates, with CBD Pink Kush achieving the highest germination response (83%) among the tested cultivars. Furthermore, cocopeat demonstrated superior performance as a seedling growth medium across all cultivars, indicating its potential for hemp cultivation practices. This pioneering study provides a baseline for understanding hemp seed germination and seedling growth under different priming and growth media conditions, offering valuable insights for future research aimed at industrial and medicinal hemp production.https://doi.org/10.1038/s41598-025-86469-yHemp cultivarsSeed primingGerminationSeedling vigorCannabis sativa |
| spellingShingle | Saba Latif Rahmatullah Qureshi Abdul Rauf NoshinIlyas Qaiser Hussain Syed Sabir Hussain Shah Sabith Rehman Arshad Mahmood Khan Nasrullah Khan Mostafa A. Abdel-Maksoud Abdul Malik Sabiha Fatima Bushra Hafeez Kiani Influence of different priming treatments on germination potential and seedling establishment of four important hemp (Cannabis sativa L.) cultivars Scientific Reports Hemp cultivars Seed priming Germination Seedling vigor Cannabis sativa |
| title | Influence of different priming treatments on germination potential and seedling establishment of four important hemp (Cannabis sativa L.) cultivars |
| title_full | Influence of different priming treatments on germination potential and seedling establishment of four important hemp (Cannabis sativa L.) cultivars |
| title_fullStr | Influence of different priming treatments on germination potential and seedling establishment of four important hemp (Cannabis sativa L.) cultivars |
| title_full_unstemmed | Influence of different priming treatments on germination potential and seedling establishment of four important hemp (Cannabis sativa L.) cultivars |
| title_short | Influence of different priming treatments on germination potential and seedling establishment of four important hemp (Cannabis sativa L.) cultivars |
| title_sort | influence of different priming treatments on germination potential and seedling establishment of four important hemp cannabis sativa l cultivars |
| topic | Hemp cultivars Seed priming Germination Seedling vigor Cannabis sativa |
| url | https://doi.org/10.1038/s41598-025-86469-y |
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