Demineralized Freeze-Dried Bovine Cortical Bone: Its Potential for Guided Bone Regeneration Membrane
Background. Bovine pericardium collagen membrane (BPCM) had been widely used in guided bone regeneration (GBR) whose manufacturing process usually required chemical cross-linking to prolong its biodegradation. However, cross-linking of collagen fibrils was associated with poorer tissue integration a...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2017/5149675 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832546112991395840 |
|---|---|
| author | David B. Kamadjaja Achmad Harijadi Pratiwi Soesilawati Eny Wahyuni Nurul Maulidah Akhsanal Fauzi Fika Rah Ayu Roberto Simanjuntak R. Soesanto Djodi Asmara Andra Rizqiawan Peter Agus Coen Pramono |
| author_facet | David B. Kamadjaja Achmad Harijadi Pratiwi Soesilawati Eny Wahyuni Nurul Maulidah Akhsanal Fauzi Fika Rah Ayu Roberto Simanjuntak R. Soesanto Djodi Asmara Andra Rizqiawan Peter Agus Coen Pramono |
| author_sort | David B. Kamadjaja |
| collection | DOAJ |
| description | Background. Bovine pericardium collagen membrane (BPCM) had been widely used in guided bone regeneration (GBR) whose manufacturing process usually required chemical cross-linking to prolong its biodegradation. However, cross-linking of collagen fibrils was associated with poorer tissue integration and delayed vascular invasion. Objective. This study evaluated the potential of bovine cortical bone collagen membrane for GBR by evaluating its antigenicity potential, cytotoxicity, immune and tissue response, and biodegradation behaviors. Material and Methods. Antigenicity potential of demineralized freeze-dried bovine cortical bone membrane (DFDBCBM) was done with histology-based anticellularity evaluation, while cytotoxicity was analyzed using MTT Assay. Evaluation of immune response, tissue response, and biodegradation was done by randomly implanting DFDBCBM and BPCM in rat’s subcutaneous dorsum. Samples were collected at 2, 5, and 7 days and 7, 14, 21, and 28 days for biocompatibility and tissue response-biodegradation study, respectively. Result. DFDBCBM, histologically, showed no retained cells; however, it showed some level of in vitro cytotoxicity. In vivo study exhibited increased immune response to DFDBCBM in early healing phase; however, normal tissue response and degradation rate were observed up to 4 weeks after DFDBCBM implantation. Conclusion. Demineralized freeze-dried bovine cortical bone membrane showed potential for clinical application; however, it needs to be optimized in its biocompatibility to fulfill all requirements for GBR membrane. |
| format | Article |
| id | doaj-art-1a17ce1f100c4dd9906b3e4352756241 |
| institution | Kabale University |
| issn | 1687-8728 1687-8736 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj-art-1a17ce1f100c4dd9906b3e43527562412025-02-03T07:23:49ZengWileyInternational Journal of Dentistry1687-87281687-87362017-01-01201710.1155/2017/51496755149675Demineralized Freeze-Dried Bovine Cortical Bone: Its Potential for Guided Bone Regeneration MembraneDavid B. Kamadjaja0Achmad Harijadi1Pratiwi Soesilawati2Eny Wahyuni3Nurul Maulidah4Akhsanal Fauzi5Fika Rah Ayu6Roberto Simanjuntak7R. Soesanto8Djodi Asmara9Andra Rizqiawan10Peter Agus11Coen Pramono12Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaDepartment of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaDepartment of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaResidency Program, Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaResidency Program, Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaResidency Program, Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaResidency Program, Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaDepartment of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaDepartment of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaDepartment of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaDepartment of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaDepartment of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaDepartment of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaBackground. Bovine pericardium collagen membrane (BPCM) had been widely used in guided bone regeneration (GBR) whose manufacturing process usually required chemical cross-linking to prolong its biodegradation. However, cross-linking of collagen fibrils was associated with poorer tissue integration and delayed vascular invasion. Objective. This study evaluated the potential of bovine cortical bone collagen membrane for GBR by evaluating its antigenicity potential, cytotoxicity, immune and tissue response, and biodegradation behaviors. Material and Methods. Antigenicity potential of demineralized freeze-dried bovine cortical bone membrane (DFDBCBM) was done with histology-based anticellularity evaluation, while cytotoxicity was analyzed using MTT Assay. Evaluation of immune response, tissue response, and biodegradation was done by randomly implanting DFDBCBM and BPCM in rat’s subcutaneous dorsum. Samples were collected at 2, 5, and 7 days and 7, 14, 21, and 28 days for biocompatibility and tissue response-biodegradation study, respectively. Result. DFDBCBM, histologically, showed no retained cells; however, it showed some level of in vitro cytotoxicity. In vivo study exhibited increased immune response to DFDBCBM in early healing phase; however, normal tissue response and degradation rate were observed up to 4 weeks after DFDBCBM implantation. Conclusion. Demineralized freeze-dried bovine cortical bone membrane showed potential for clinical application; however, it needs to be optimized in its biocompatibility to fulfill all requirements for GBR membrane.http://dx.doi.org/10.1155/2017/5149675 |
| spellingShingle | David B. Kamadjaja Achmad Harijadi Pratiwi Soesilawati Eny Wahyuni Nurul Maulidah Akhsanal Fauzi Fika Rah Ayu Roberto Simanjuntak R. Soesanto Djodi Asmara Andra Rizqiawan Peter Agus Coen Pramono Demineralized Freeze-Dried Bovine Cortical Bone: Its Potential for Guided Bone Regeneration Membrane International Journal of Dentistry |
| title | Demineralized Freeze-Dried Bovine Cortical Bone: Its Potential for Guided Bone Regeneration Membrane |
| title_full | Demineralized Freeze-Dried Bovine Cortical Bone: Its Potential for Guided Bone Regeneration Membrane |
| title_fullStr | Demineralized Freeze-Dried Bovine Cortical Bone: Its Potential for Guided Bone Regeneration Membrane |
| title_full_unstemmed | Demineralized Freeze-Dried Bovine Cortical Bone: Its Potential for Guided Bone Regeneration Membrane |
| title_short | Demineralized Freeze-Dried Bovine Cortical Bone: Its Potential for Guided Bone Regeneration Membrane |
| title_sort | demineralized freeze dried bovine cortical bone its potential for guided bone regeneration membrane |
| url | http://dx.doi.org/10.1155/2017/5149675 |
| work_keys_str_mv | AT davidbkamadjaja demineralizedfreezedriedbovinecorticalboneitspotentialforguidedboneregenerationmembrane AT achmadharijadi demineralizedfreezedriedbovinecorticalboneitspotentialforguidedboneregenerationmembrane AT pratiwisoesilawati demineralizedfreezedriedbovinecorticalboneitspotentialforguidedboneregenerationmembrane AT enywahyuni demineralizedfreezedriedbovinecorticalboneitspotentialforguidedboneregenerationmembrane AT nurulmaulidah demineralizedfreezedriedbovinecorticalboneitspotentialforguidedboneregenerationmembrane AT akhsanalfauzi demineralizedfreezedriedbovinecorticalboneitspotentialforguidedboneregenerationmembrane AT fikarahayu demineralizedfreezedriedbovinecorticalboneitspotentialforguidedboneregenerationmembrane AT robertosimanjuntak demineralizedfreezedriedbovinecorticalboneitspotentialforguidedboneregenerationmembrane AT rsoesanto demineralizedfreezedriedbovinecorticalboneitspotentialforguidedboneregenerationmembrane AT djodiasmara demineralizedfreezedriedbovinecorticalboneitspotentialforguidedboneregenerationmembrane AT andrarizqiawan demineralizedfreezedriedbovinecorticalboneitspotentialforguidedboneregenerationmembrane AT peteragus demineralizedfreezedriedbovinecorticalboneitspotentialforguidedboneregenerationmembrane AT coenpramono demineralizedfreezedriedbovinecorticalboneitspotentialforguidedboneregenerationmembrane |