Evaluation of a user‐friendly CSDS cage apparatus for studying depressive‐like behaviors in rodents
Abstract Background Previously, a chronic social defeat stress (CSDS) model has been widely‐adopted for assessing depressive‐like behaviors in animals. However, there is still room for improvement in the CSDS model to safeguard study accuracy and the welfare of lab rodents. Our study team developed...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-01-01
|
| Series: | Animal Models and Experimental Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ame2.12510 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832087666646056960 |
|---|---|
| author | Hao Zhang Dongmei Gao Minghu Hu Wanqing Zhou Muxuan Han Ya Sun Yang Zhang Jieqiong Wang Mingzhou Gao |
| author_facet | Hao Zhang Dongmei Gao Minghu Hu Wanqing Zhou Muxuan Han Ya Sun Yang Zhang Jieqiong Wang Mingzhou Gao |
| author_sort | Hao Zhang |
| collection | DOAJ |
| description | Abstract Background Previously, a chronic social defeat stress (CSDS) model has been widely‐adopted for assessing depressive‐like behaviors in animals. However, there is still room for improvement in the CSDS model to safeguard study accuracy and the welfare of lab rodents. Our study team developed a novel, standardized apparatus to induce CSDS in rodents and assessed the model's practical adaptability. Methods An innovative CSDS cage apparatus and water bottle was designed. To evaluate the effectiveness of the newly developed tools, a variety of animal models, including the tail suspension test (TST), sucrose preference test, forced swimming test (FST), novelty‐suppressed feeding test, female urine sniffing test, and open field test (OFT), were adopted to assess depressive‐like behaviors in mice. Fluoxetine treatment was also administered to observe the reversal effect, as part of the validation. Results The CSDS cage apparatus resulted in the manifestation of depressive‐like behaviors in the model mice. Significant reductions in sucrose preference and urine sniffing time were observed, while the OFT revealed decreased central zone total distance, residence time, and frequency of entry. Moreover, increased immobility was found in the FST and TST. Fluoxetine treatment was found to successfully reverse the modeling effect. Conclusion The CSDS cage apparatus was validated for enhanced usability and addressed the previous challenges of water bottle leakage and lab rodent welfare issues. The consistent results from multiple behavioral tests also supported real‐world application of the apparatus, offering researchers a promising alternative to conventional rodent cages. |
| format | Article |
| id | doaj-art-1a070b67b0114eeeb6bc0e6ffa6b1c28 |
| institution | Kabale University |
| issn | 2576-2095 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Animal Models and Experimental Medicine |
| spelling | doaj-art-1a070b67b0114eeeb6bc0e6ffa6b1c282025-02-06T03:52:56ZengWileyAnimal Models and Experimental Medicine2576-20952025-01-018117918610.1002/ame2.12510Evaluation of a user‐friendly CSDS cage apparatus for studying depressive‐like behaviors in rodentsHao Zhang0Dongmei Gao1Minghu Hu2Wanqing Zhou3Muxuan Han4Ya Sun5Yang Zhang6Jieqiong Wang7Mingzhou Gao8High‐Level Key Disciplines of Traditional Chinese Medicine: Basic Theory of Traditional Chinese Medicine, National Administration of Traditional Chinese Medicine Shandong University of Traditional Chinese Medicine Jinan ChinaHigh‐Level Key Disciplines of Traditional Chinese Medicine: Basic Theory of Traditional Chinese Medicine, National Administration of Traditional Chinese Medicine Shandong University of Traditional Chinese Medicine Jinan ChinaExperimental Center Shandong University of Traditional Chinese Medicine Jinan Shandong ChinaExperimental Center Shandong University of Traditional Chinese Medicine Jinan Shandong ChinaExperimental Center Shandong University of Traditional Chinese Medicine Jinan Shandong ChinaInnovative Institute of Chinese Medicine and Pharmacy Shandong University of Traditional Chinese Medicine Jinan Shandong ChinaInnovative Institute of Chinese Medicine and Pharmacy Shandong University of Traditional Chinese Medicine Jinan Shandong ChinaSocial Cooperation and Achievement Transformation Department Shandong University of Traditional Chinese Medicine Jinan Shandong ChinaHigh‐Level Key Disciplines of Traditional Chinese Medicine: Basic Theory of Traditional Chinese Medicine, National Administration of Traditional Chinese Medicine Shandong University of Traditional Chinese Medicine Jinan ChinaAbstract Background Previously, a chronic social defeat stress (CSDS) model has been widely‐adopted for assessing depressive‐like behaviors in animals. However, there is still room for improvement in the CSDS model to safeguard study accuracy and the welfare of lab rodents. Our study team developed a novel, standardized apparatus to induce CSDS in rodents and assessed the model's practical adaptability. Methods An innovative CSDS cage apparatus and water bottle was designed. To evaluate the effectiveness of the newly developed tools, a variety of animal models, including the tail suspension test (TST), sucrose preference test, forced swimming test (FST), novelty‐suppressed feeding test, female urine sniffing test, and open field test (OFT), were adopted to assess depressive‐like behaviors in mice. Fluoxetine treatment was also administered to observe the reversal effect, as part of the validation. Results The CSDS cage apparatus resulted in the manifestation of depressive‐like behaviors in the model mice. Significant reductions in sucrose preference and urine sniffing time were observed, while the OFT revealed decreased central zone total distance, residence time, and frequency of entry. Moreover, increased immobility was found in the FST and TST. Fluoxetine treatment was found to successfully reverse the modeling effect. Conclusion The CSDS cage apparatus was validated for enhanced usability and addressed the previous challenges of water bottle leakage and lab rodent welfare issues. The consistent results from multiple behavioral tests also supported real‐world application of the apparatus, offering researchers a promising alternative to conventional rodent cages.https://doi.org/10.1002/ame2.12510CSDSdepressionexperimental cageleak‐proof water bottlemethodology |
| spellingShingle | Hao Zhang Dongmei Gao Minghu Hu Wanqing Zhou Muxuan Han Ya Sun Yang Zhang Jieqiong Wang Mingzhou Gao Evaluation of a user‐friendly CSDS cage apparatus for studying depressive‐like behaviors in rodents Animal Models and Experimental Medicine CSDS depression experimental cage leak‐proof water bottle methodology |
| title | Evaluation of a user‐friendly CSDS cage apparatus for studying depressive‐like behaviors in rodents |
| title_full | Evaluation of a user‐friendly CSDS cage apparatus for studying depressive‐like behaviors in rodents |
| title_fullStr | Evaluation of a user‐friendly CSDS cage apparatus for studying depressive‐like behaviors in rodents |
| title_full_unstemmed | Evaluation of a user‐friendly CSDS cage apparatus for studying depressive‐like behaviors in rodents |
| title_short | Evaluation of a user‐friendly CSDS cage apparatus for studying depressive‐like behaviors in rodents |
| title_sort | evaluation of a user friendly csds cage apparatus for studying depressive like behaviors in rodents |
| topic | CSDS depression experimental cage leak‐proof water bottle methodology |
| url | https://doi.org/10.1002/ame2.12510 |
| work_keys_str_mv | AT haozhang evaluationofauserfriendlycsdscageapparatusforstudyingdepressivelikebehaviorsinrodents AT dongmeigao evaluationofauserfriendlycsdscageapparatusforstudyingdepressivelikebehaviorsinrodents AT minghuhu evaluationofauserfriendlycsdscageapparatusforstudyingdepressivelikebehaviorsinrodents AT wanqingzhou evaluationofauserfriendlycsdscageapparatusforstudyingdepressivelikebehaviorsinrodents AT muxuanhan evaluationofauserfriendlycsdscageapparatusforstudyingdepressivelikebehaviorsinrodents AT yasun evaluationofauserfriendlycsdscageapparatusforstudyingdepressivelikebehaviorsinrodents AT yangzhang evaluationofauserfriendlycsdscageapparatusforstudyingdepressivelikebehaviorsinrodents AT jieqiongwang evaluationofauserfriendlycsdscageapparatusforstudyingdepressivelikebehaviorsinrodents AT mingzhougao evaluationofauserfriendlycsdscageapparatusforstudyingdepressivelikebehaviorsinrodents |