Multi-omics elucidates the kidney damage caused by aquatic Cu via the gut-kidney axis in ducks
Copper (Cu) is an essential trace element for biological growth and development. Excessive intake of Cu exists harmful effects on organisms. However, whether excessive Cu intake induces kidney function damage by gut microbiota regulation remains unclear. Ducks are important species of waterfowl that...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-02-01
|
| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325001800 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832087666161614848 |
|---|---|
| author | Mingcai Duan Jinyu Liu Zhaoxia Cai Li Chen Yong Tian Wenwu Xu Tao Zeng Tiantian Gu Lizhi Lu |
| author_facet | Mingcai Duan Jinyu Liu Zhaoxia Cai Li Chen Yong Tian Wenwu Xu Tao Zeng Tiantian Gu Lizhi Lu |
| author_sort | Mingcai Duan |
| collection | DOAJ |
| description | Copper (Cu) is an essential trace element for biological growth and development. Excessive intake of Cu exists harmful effects on organisms. However, whether excessive Cu intake induces kidney function damage by gut microbiota regulation remains unclear. Ducks are important species of waterfowl that are often exposed to Cu contamination in water sources. In this study, we aim to elucidate the effects of Cu exposure on renal inflammation through the gut-kidney axis in ducks. The ducks were gavaged with different doses of CuSO4 (0, 100, and 200 mg/kg body weight) for 4 weeks. Results indicate that Cu exposure causes pathological damage to the kidney, with a significant increase in the levels of TNFα, IL-6, and IL-1β in both serum and renal tissue. 16S rDNA analysis revealed that the relative abundances of Candidatus_Saccharimonas and Bacteroides were significantly reduced in the Cu-induced group. Transcriptomic analysis of kidney tissue reveals that following Cu exposure, 30 genes show significant differential expression. GO and KEGG enrichment analyses were most involved in Interleukin-1 Receptor Activity, Taurine and hypotaurine metabolism, Nitrogen metabolism, and Proximal tubule bicarbonate reclamation. Metabolomic analysis revealed that 28 metabolites are present in both kidney tissue and cecal contents. Correlation analysis revealed a strong correlation among 5 common metabolites: Aminoglutethimide, Boscalid, Dantrolene, Cer[ns] d34:1, and Stearidonic acid. In the cecum, these five metabolites are closely associated with 26 intestinal microorganisms, including Bacteroides, Candidatus_Saccharimonas, and Colidextribacter. In the kidney, apart from Stearidonic acid, the other four metabolites are closely correlated with genes such as FOS, and IL1RL1. Overall, our study indicates that excessive Cu induces significant kidney inflammation, the metabolites alteration and gut microbiota disorders. These findings shed light on the underlying mechanisms of Cu-induced kidney damage via the indirect pathway of the gut-kidney axis. |
| format | Article |
| id | doaj-art-1a0687e3b0a54489b2c17e44bedf7670 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-1a0687e3b0a54489b2c17e44bedf76702025-02-06T05:10:58ZengElsevierEcotoxicology and Environmental Safety0147-65132025-02-01291117844Multi-omics elucidates the kidney damage caused by aquatic Cu via the gut-kidney axis in ducksMingcai Duan0Jinyu Liu1Zhaoxia Cai2Li Chen3Yong Tian4Wenwu Xu5Tao Zeng6Tiantian Gu7Lizhi Lu8Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs of China, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; Hubei Hongshan Laboratory, National Research and Development Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs of China, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaHubei Hongshan Laboratory, National Research and Development Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs of China, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaKey Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs of China, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaKey Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs of China, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaKey Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs of China, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaKey Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs of China, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; Corresponding authors.Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs of China, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; Corresponding authors.Copper (Cu) is an essential trace element for biological growth and development. Excessive intake of Cu exists harmful effects on organisms. However, whether excessive Cu intake induces kidney function damage by gut microbiota regulation remains unclear. Ducks are important species of waterfowl that are often exposed to Cu contamination in water sources. In this study, we aim to elucidate the effects of Cu exposure on renal inflammation through the gut-kidney axis in ducks. The ducks were gavaged with different doses of CuSO4 (0, 100, and 200 mg/kg body weight) for 4 weeks. Results indicate that Cu exposure causes pathological damage to the kidney, with a significant increase in the levels of TNFα, IL-6, and IL-1β in both serum and renal tissue. 16S rDNA analysis revealed that the relative abundances of Candidatus_Saccharimonas and Bacteroides were significantly reduced in the Cu-induced group. Transcriptomic analysis of kidney tissue reveals that following Cu exposure, 30 genes show significant differential expression. GO and KEGG enrichment analyses were most involved in Interleukin-1 Receptor Activity, Taurine and hypotaurine metabolism, Nitrogen metabolism, and Proximal tubule bicarbonate reclamation. Metabolomic analysis revealed that 28 metabolites are present in both kidney tissue and cecal contents. Correlation analysis revealed a strong correlation among 5 common metabolites: Aminoglutethimide, Boscalid, Dantrolene, Cer[ns] d34:1, and Stearidonic acid. In the cecum, these five metabolites are closely associated with 26 intestinal microorganisms, including Bacteroides, Candidatus_Saccharimonas, and Colidextribacter. In the kidney, apart from Stearidonic acid, the other four metabolites are closely correlated with genes such as FOS, and IL1RL1. Overall, our study indicates that excessive Cu induces significant kidney inflammation, the metabolites alteration and gut microbiota disorders. These findings shed light on the underlying mechanisms of Cu-induced kidney damage via the indirect pathway of the gut-kidney axis.http://www.sciencedirect.com/science/article/pii/S0147651325001800CuRenal inflammationGut microbiotaTranscriptomeGut-kidney axisDuck |
| spellingShingle | Mingcai Duan Jinyu Liu Zhaoxia Cai Li Chen Yong Tian Wenwu Xu Tao Zeng Tiantian Gu Lizhi Lu Multi-omics elucidates the kidney damage caused by aquatic Cu via the gut-kidney axis in ducks Ecotoxicology and Environmental Safety Cu Renal inflammation Gut microbiota Transcriptome Gut-kidney axis Duck |
| title | Multi-omics elucidates the kidney damage caused by aquatic Cu via the gut-kidney axis in ducks |
| title_full | Multi-omics elucidates the kidney damage caused by aquatic Cu via the gut-kidney axis in ducks |
| title_fullStr | Multi-omics elucidates the kidney damage caused by aquatic Cu via the gut-kidney axis in ducks |
| title_full_unstemmed | Multi-omics elucidates the kidney damage caused by aquatic Cu via the gut-kidney axis in ducks |
| title_short | Multi-omics elucidates the kidney damage caused by aquatic Cu via the gut-kidney axis in ducks |
| title_sort | multi omics elucidates the kidney damage caused by aquatic cu via the gut kidney axis in ducks |
| topic | Cu Renal inflammation Gut microbiota Transcriptome Gut-kidney axis Duck |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325001800 |
| work_keys_str_mv | AT mingcaiduan multiomicselucidatesthekidneydamagecausedbyaquaticcuviathegutkidneyaxisinducks AT jinyuliu multiomicselucidatesthekidneydamagecausedbyaquaticcuviathegutkidneyaxisinducks AT zhaoxiacai multiomicselucidatesthekidneydamagecausedbyaquaticcuviathegutkidneyaxisinducks AT lichen multiomicselucidatesthekidneydamagecausedbyaquaticcuviathegutkidneyaxisinducks AT yongtian multiomicselucidatesthekidneydamagecausedbyaquaticcuviathegutkidneyaxisinducks AT wenwuxu multiomicselucidatesthekidneydamagecausedbyaquaticcuviathegutkidneyaxisinducks AT taozeng multiomicselucidatesthekidneydamagecausedbyaquaticcuviathegutkidneyaxisinducks AT tiantiangu multiomicselucidatesthekidneydamagecausedbyaquaticcuviathegutkidneyaxisinducks AT lizhilu multiomicselucidatesthekidneydamagecausedbyaquaticcuviathegutkidneyaxisinducks |