Decolourization Kinetics of Congo Red Using Immobilized and Free Cells of Rhodococcus biphenylivorans
Abstract In the present study, a cost-effective and eco-friendly approach is being explored to remove the harmful dye Congo red from synthetic wastewater, using an equatorial strain of Rhodococcus biphenylivorans. The immobilized cells’ Congo red-decolorizing activity in calcium alginate showed an i...
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Instituto de Tecnologia do Paraná (Tecpar)
2025-02-01
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| Series: | Brazilian Archives of Biology and Technology |
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| author | Maegala Nallapan Maniyam Primeela Gunalan Hazeeq Hazman Azman Hasdianty Abdullah Nor Suhaila Yaacob |
| author_facet | Maegala Nallapan Maniyam Primeela Gunalan Hazeeq Hazman Azman Hasdianty Abdullah Nor Suhaila Yaacob |
| author_sort | Maegala Nallapan Maniyam |
| collection | DOAJ |
| description | Abstract In the present study, a cost-effective and eco-friendly approach is being explored to remove the harmful dye Congo red from synthetic wastewater, using an equatorial strain of Rhodococcus biphenylivorans. The immobilized cells’ Congo red-decolorizing activity in calcium alginate showed an increase of 217% compared to the free cells. By improving the alginate concentration to 4% (w/v), the bead diameter to 3 mm and using 50 beads, a 67% decrease in the time required for complete decolorization was accomplished. Both free and immobilized cells followed first-order kinetics for the elimination of Congo red, with immobilized cells displaying a decolorization rate 3.2 times greater than that of free cells, resulting in an 84% reduction in half-life. The immobilized cells displayed the capability to remove 0.10 g/L Congo red without any significant decline in their efficiency for up to twelve cycles, offering an economic advantage due to their reusability. Examining the decolorized products of Congo red showed no hindrance in the germination of Triticum aestivum and Vigna radiata, indicating the effective removal of all toxic components from the original dye via the biological method. Therefore, this strain has significant potential as a biological agent to eliminate wastewater contaminated with Congo red efficiently. |
| format | Article |
| id | doaj-art-1a00eafec48049c59a2b14cd5e10010f |
| institution | Kabale University |
| issn | 1678-4324 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj-art-1a00eafec48049c59a2b14cd5e10010f2025-02-04T07:40:38ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242025-02-016810.1590/1678-4324-2025231220Decolourization Kinetics of Congo Red Using Immobilized and Free Cells of Rhodococcus biphenylivoransMaegala Nallapan Maniyamhttps://orcid.org/0000-0001-5670-3061Primeela Gunalanhttps://orcid.org/0009-0009-9051-9968Hazeeq Hazman Azmanhttps://orcid.org/0000-0003-4058-2378Hasdianty Abdullahhttps://orcid.org/0000-0002-2669-0121Nor Suhaila Yaacobhttps://orcid.org/0000-0002-5582-4715Abstract In the present study, a cost-effective and eco-friendly approach is being explored to remove the harmful dye Congo red from synthetic wastewater, using an equatorial strain of Rhodococcus biphenylivorans. The immobilized cells’ Congo red-decolorizing activity in calcium alginate showed an increase of 217% compared to the free cells. By improving the alginate concentration to 4% (w/v), the bead diameter to 3 mm and using 50 beads, a 67% decrease in the time required for complete decolorization was accomplished. Both free and immobilized cells followed first-order kinetics for the elimination of Congo red, with immobilized cells displaying a decolorization rate 3.2 times greater than that of free cells, resulting in an 84% reduction in half-life. The immobilized cells displayed the capability to remove 0.10 g/L Congo red without any significant decline in their efficiency for up to twelve cycles, offering an economic advantage due to their reusability. Examining the decolorized products of Congo red showed no hindrance in the germination of Triticum aestivum and Vigna radiata, indicating the effective removal of all toxic components from the original dye via the biological method. Therefore, this strain has significant potential as a biological agent to eliminate wastewater contaminated with Congo red efficiently.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132025000100700&lng=en&tlng=enCongo reddecolorizationimmobilizationkinetic studyRhodococcus biphenylivorans |
| spellingShingle | Maegala Nallapan Maniyam Primeela Gunalan Hazeeq Hazman Azman Hasdianty Abdullah Nor Suhaila Yaacob Decolourization Kinetics of Congo Red Using Immobilized and Free Cells of Rhodococcus biphenylivorans Brazilian Archives of Biology and Technology Congo red decolorization immobilization kinetic study Rhodococcus biphenylivorans |
| title | Decolourization Kinetics of Congo Red Using Immobilized and Free Cells of Rhodococcus biphenylivorans |
| title_full | Decolourization Kinetics of Congo Red Using Immobilized and Free Cells of Rhodococcus biphenylivorans |
| title_fullStr | Decolourization Kinetics of Congo Red Using Immobilized and Free Cells of Rhodococcus biphenylivorans |
| title_full_unstemmed | Decolourization Kinetics of Congo Red Using Immobilized and Free Cells of Rhodococcus biphenylivorans |
| title_short | Decolourization Kinetics of Congo Red Using Immobilized and Free Cells of Rhodococcus biphenylivorans |
| title_sort | decolourization kinetics of congo red using immobilized and free cells of rhodococcus biphenylivorans |
| topic | Congo red decolorization immobilization kinetic study Rhodococcus biphenylivorans |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132025000100700&lng=en&tlng=en |
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