Development of a Confocal Optical System Design for Molecular Imaging Applications of Biochip
A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 ma...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2007-01-01
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| Series: | International Journal of Biomedical Imaging |
| Online Access: | http://dx.doi.org/10.1155/2007/79710 |
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| _version_ | 1832549914620461056 |
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| author | Guoliang Huang Shukuan Xu Jiang Zhu Cheng Deng Zhonghua Dong Yang Yang Xiaoyong Yang Xianhua Wang Guofan Jin |
| author_facet | Guoliang Huang Shukuan Xu Jiang Zhu Cheng Deng Zhonghua Dong Yang Yang Xiaoyong Yang Xianhua Wang Guofan Jin |
| author_sort | Guoliang Huang |
| collection | DOAJ |
| description | A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used. This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners. The scanner is as equally good for the molecular imaging detection of enclosed biochips as for the detection of biological samples on a slide surface covered with a cover-slip glass. Some applications of gene and protein imagings using the dual laser confocal scanner are described. |
| format | Article |
| id | doaj-art-19d8d8b5df0341cab56f22ab7b8dcb40 |
| institution | Kabale University |
| issn | 1687-4188 1687-4196 |
| language | English |
| publishDate | 2007-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomedical Imaging |
| spelling | doaj-art-19d8d8b5df0341cab56f22ab7b8dcb402025-02-03T06:08:14ZengWileyInternational Journal of Biomedical Imaging1687-41881687-41962007-01-01200710.1155/2007/7971079710Development of a Confocal Optical System Design for Molecular Imaging Applications of BiochipGuoliang Huang0Shukuan Xu1Jiang Zhu2Cheng Deng3Zhonghua Dong4Yang Yang5Xiaoyong Yang6Xianhua Wang7Guofan Jin8Medical Systems Biology Research Center, School of Medicine, Tsinghua University, Beijing 100084, ChinaMedical Systems Biology Research Center, School of Medicine, Tsinghua University, Beijing 100084, ChinaMedical Systems Biology Research Center, School of Medicine, Tsinghua University, Beijing 100084, ChinaMedical Systems Biology Research Center, School of Medicine, Tsinghua University, Beijing 100084, ChinaMedical Systems Biology Research Center, School of Medicine, Tsinghua University, Beijing 100084, ChinaMedical Systems Biology Research Center, School of Medicine, Tsinghua University, Beijing 100084, ChinaNational Engineering Research Center for Beijing Biochip Technology, Beijing 102206, ChinaNational Engineering Research Center for Beijing Biochip Technology, Beijing 102206, ChinaDepartment of Precision Instruments and Mechanology, School of Mechanical Engineering, Tsinghua University, Beijing 100084, ChinaA novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used. This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners. The scanner is as equally good for the molecular imaging detection of enclosed biochips as for the detection of biological samples on a slide surface covered with a cover-slip glass. Some applications of gene and protein imagings using the dual laser confocal scanner are described.http://dx.doi.org/10.1155/2007/79710 |
| spellingShingle | Guoliang Huang Shukuan Xu Jiang Zhu Cheng Deng Zhonghua Dong Yang Yang Xiaoyong Yang Xianhua Wang Guofan Jin Development of a Confocal Optical System Design for Molecular Imaging Applications of Biochip International Journal of Biomedical Imaging |
| title | Development of a Confocal Optical System Design for Molecular Imaging Applications of Biochip |
| title_full | Development of a Confocal Optical System Design for Molecular Imaging Applications of Biochip |
| title_fullStr | Development of a Confocal Optical System Design for Molecular Imaging Applications of Biochip |
| title_full_unstemmed | Development of a Confocal Optical System Design for Molecular Imaging Applications of Biochip |
| title_short | Development of a Confocal Optical System Design for Molecular Imaging Applications of Biochip |
| title_sort | development of a confocal optical system design for molecular imaging applications of biochip |
| url | http://dx.doi.org/10.1155/2007/79710 |
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