Mitigation of PFOA/PFOS toxicity in zebrafish (Danio rerio) by oxidative stress modulation and gut microbial metabolism through the use of aquatic probiotic Lactobacillus rhamnosus
Perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) have been classified as typical persistent organic pollutants (POPs), thus the residues and risks in the environment should not be underestimated. In order to ensure healthy aquaculture process and safe aquatic products, it is impera...
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| Format: | Article |
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KeAi Communications Co., Ltd.
2025-01-01
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| Series: | Water Cycle |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266644532400031X |
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| author | Chun Wang Guanyi Li Sibao Chen Ruixuan Li Mengyan Wang Xia Wang Ning Ding Yingxue Sun |
| author_facet | Chun Wang Guanyi Li Sibao Chen Ruixuan Li Mengyan Wang Xia Wang Ning Ding Yingxue Sun |
| author_sort | Chun Wang |
| collection | DOAJ |
| description | Perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) have been classified as typical persistent organic pollutants (POPs), thus the residues and risks in the environment should not be underestimated. In order to ensure healthy aquaculture process and safe aquatic products, it is imperative to explore methods that can effectively mitigate the toxicity of PFOS and PFOA on fish. Research has demonstrated that Lactobacillus rhamnosus can ameliorate the toxic impacts induced by perfluorobutyric acid (PFBS) on zebrafish through regulation of host intestinal microbiota imbalance and enhancement of host antioxidant capacity. However, the potential ameliorative effects of probiotics on the toxic effects caused by PFOA and PFOS in zebrafish have not been studied. In this study, we conducted innovative research to examine the protective effects of L. rhamnosus against PFOA- and PFOS-induced oxidative damage, neurotoxicity, and disruption of intestinal microflora in adult zebrafish. The results demonstrated that L. rhamnosus effectively mitigated the oxidative stress induced by PFOA and PFOS in zebrafish through modulation of oxidative stress indicators (superoxide dismutase, SOD, lactate dehydrogenase, LDH, and catalase, CAT). The impact of PFOA and PFOS on intestinal tissue damage followed the order: PFOA + PFOS > PFOS > PFOA. Additionally, L. rhamnosus exhibited significant reparative effects on damaged intestinal tissues, as evidenced by the restoration of damaged intestinal villi and an increase in lymphocyte count. Furthermore, it was capable of enhancing the abundance of thick-walled phylum and actinomycetes phylum within the zebrafish gut microbiota. Notably, through the analysis of metabolic pathway differences, it was observed that the PFOA-exposure group exhibited a higher proportion of Rhodococcus in the PWY-7315 pathway, whereas L. rhamnosus mitigated this trend. These novel findings not only elucidate the mechanism by which L. rhamnosus efficiently reduces PFOS and PFOA toxicity in zebrafish but also provide a theoretical basis and practical recommendations for utilizing probiotics in aquaculture. |
| format | Article |
| id | doaj-art-19c8016507594c038748a92e24716f0c |
| institution | Kabale University |
| issn | 2666-4453 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Water Cycle |
| spelling | doaj-art-19c8016507594c038748a92e24716f0c2025-02-06T05:12:52ZengKeAi Communications Co., Ltd.Water Cycle2666-44532025-01-0167181Mitigation of PFOA/PFOS toxicity in zebrafish (Danio rerio) by oxidative stress modulation and gut microbial metabolism through the use of aquatic probiotic Lactobacillus rhamnosusChun Wang0Guanyi Li1Sibao Chen2Ruixuan Li3Mengyan Wang4Xia Wang5Ning Ding6Yingxue Sun7Department of Environmental Science and Engineering, Beijing Technology and Business University, Beijing, 100048, ChinaDepartment of Environmental Science and Engineering, Beijing Technology and Business University, Beijing, 100048, ChinaChangjiang Institute of Survey Planning Design and Research, Key Laboratory of Changjiang Regulation and Protection of Ministry of Water Resources, Wuhan, 430010, ChinaDepartment of Environmental Science and Engineering, Beijing Technology and Business University, Beijing, 100048, ChinaDepartment of Environmental Science and Engineering, Beijing Technology and Business University, Beijing, 100048, ChinaDepartment of Environmental Science and Engineering, Beijing Technology and Business University, Beijing, 100048, ChinaDepartment of Environmental Science and Engineering, Beijing Technology and Business University, Beijing, 100048, China; Corresponding author.Department of Environmental Science and Engineering, Beijing Technology and Business University, Beijing, 100048, ChinaPerfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) have been classified as typical persistent organic pollutants (POPs), thus the residues and risks in the environment should not be underestimated. In order to ensure healthy aquaculture process and safe aquatic products, it is imperative to explore methods that can effectively mitigate the toxicity of PFOS and PFOA on fish. Research has demonstrated that Lactobacillus rhamnosus can ameliorate the toxic impacts induced by perfluorobutyric acid (PFBS) on zebrafish through regulation of host intestinal microbiota imbalance and enhancement of host antioxidant capacity. However, the potential ameliorative effects of probiotics on the toxic effects caused by PFOA and PFOS in zebrafish have not been studied. In this study, we conducted innovative research to examine the protective effects of L. rhamnosus against PFOA- and PFOS-induced oxidative damage, neurotoxicity, and disruption of intestinal microflora in adult zebrafish. The results demonstrated that L. rhamnosus effectively mitigated the oxidative stress induced by PFOA and PFOS in zebrafish through modulation of oxidative stress indicators (superoxide dismutase, SOD, lactate dehydrogenase, LDH, and catalase, CAT). The impact of PFOA and PFOS on intestinal tissue damage followed the order: PFOA + PFOS > PFOS > PFOA. Additionally, L. rhamnosus exhibited significant reparative effects on damaged intestinal tissues, as evidenced by the restoration of damaged intestinal villi and an increase in lymphocyte count. Furthermore, it was capable of enhancing the abundance of thick-walled phylum and actinomycetes phylum within the zebrafish gut microbiota. Notably, through the analysis of metabolic pathway differences, it was observed that the PFOA-exposure group exhibited a higher proportion of Rhodococcus in the PWY-7315 pathway, whereas L. rhamnosus mitigated this trend. These novel findings not only elucidate the mechanism by which L. rhamnosus efficiently reduces PFOS and PFOA toxicity in zebrafish but also provide a theoretical basis and practical recommendations for utilizing probiotics in aquaculture.http://www.sciencedirect.com/science/article/pii/S266644532400031XPFOAPFOSLactobacillus rhamnosusOxidative stressGut microbiota |
| spellingShingle | Chun Wang Guanyi Li Sibao Chen Ruixuan Li Mengyan Wang Xia Wang Ning Ding Yingxue Sun Mitigation of PFOA/PFOS toxicity in zebrafish (Danio rerio) by oxidative stress modulation and gut microbial metabolism through the use of aquatic probiotic Lactobacillus rhamnosus Water Cycle PFOA PFOS Lactobacillus rhamnosus Oxidative stress Gut microbiota |
| title | Mitigation of PFOA/PFOS toxicity in zebrafish (Danio rerio) by oxidative stress modulation and gut microbial metabolism through the use of aquatic probiotic Lactobacillus rhamnosus |
| title_full | Mitigation of PFOA/PFOS toxicity in zebrafish (Danio rerio) by oxidative stress modulation and gut microbial metabolism through the use of aquatic probiotic Lactobacillus rhamnosus |
| title_fullStr | Mitigation of PFOA/PFOS toxicity in zebrafish (Danio rerio) by oxidative stress modulation and gut microbial metabolism through the use of aquatic probiotic Lactobacillus rhamnosus |
| title_full_unstemmed | Mitigation of PFOA/PFOS toxicity in zebrafish (Danio rerio) by oxidative stress modulation and gut microbial metabolism through the use of aquatic probiotic Lactobacillus rhamnosus |
| title_short | Mitigation of PFOA/PFOS toxicity in zebrafish (Danio rerio) by oxidative stress modulation and gut microbial metabolism through the use of aquatic probiotic Lactobacillus rhamnosus |
| title_sort | mitigation of pfoa pfos toxicity in zebrafish danio rerio by oxidative stress modulation and gut microbial metabolism through the use of aquatic probiotic lactobacillus rhamnosus |
| topic | PFOA PFOS Lactobacillus rhamnosus Oxidative stress Gut microbiota |
| url | http://www.sciencedirect.com/science/article/pii/S266644532400031X |
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