Small RNA sequencing highlights potential functions of tsRNAs in regulating pyroptosis in trigeminal neuralgia
The mechanism of pyroptosis in trigeminal neuralgia (TN) progression remains unclear. The discovery of tRNA-derived small RNAs (tsRNAs) in the mechanism of pyroptosis is a breakthrough. We aim to study whether tsRNA is involved in TN progression through pyroptosis. A TN rat model was constructed by...
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Taylor & Francis Group
2024-12-01
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| Online Access: | http://dx.doi.org/10.1080/26895293.2024.2381679 |
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| author | Mizhen Qiu Fei Zeng Yi Yan Mu Xu Xuexue Zhang Zhijian Wang Yutong Wang Daying Zhang Shibiao Chen |
| author_facet | Mizhen Qiu Fei Zeng Yi Yan Mu Xu Xuexue Zhang Zhijian Wang Yutong Wang Daying Zhang Shibiao Chen |
| author_sort | Mizhen Qiu |
| collection | DOAJ |
| description | The mechanism of pyroptosis in trigeminal neuralgia (TN) progression remains unclear. The discovery of tRNA-derived small RNAs (tsRNAs) in the mechanism of pyroptosis is a breakthrough. We aim to study whether tsRNA is involved in TN progression through pyroptosis. A TN rat model was constructed by chronic constriction injury of infraorbital nerve and then mechanical hyperalgesia was quantitated using a withdrawal threshold test. Mechanical hyperalgesia was significantly elevated by constriction injury. Immunofluorescence revealed that expression of GSDMD and NLRP3 were significantly enhanced in TN rats. The expression of caspase-1, IL-1β, and IL-18 was significantly boosted after TN induction. CoPP + H2O2 exposure induced cell injury and pyroptosis of rat Schwann RSC96 cells, but reversed by VX-765 (a caspase-1 inhibitor). A total of 87 differentially expressed tsRNAs (DEtsRNAs) were identified in TN rats. KEGG pathway enrichment showed that DEtsRNAs were mainly involved in pyroptosis-related pathways, such as MAPK, ErbB, and mTOR signaling pathways. RT-qPCR showed that pyroptosis-related DEtsRNAs of tsRNA003489, tsRNA001840, and tsRNA003424 were downregulated in TN. Network analysis indicated tsRNA003489 and tsRNA001840 interact with NLRP3, a key regulator of pyroptosis. tsRNA has potential to participate in TN progression by targeting pyroptosis-related genes, which provides a new perspective for understanding pathology of TN. |
| format | Article |
| id | doaj-art-19c54869b08341edb39f7e56d29e97c5 |
| institution | Kabale University |
| issn | 2689-5307 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
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| spelling | doaj-art-19c54869b08341edb39f7e56d29e97c52025-01-20T14:38:00ZengTaylor & Francis GroupAll Life2689-53072024-12-01170110.1080/26895293.2024.23816792381679Small RNA sequencing highlights potential functions of tsRNAs in regulating pyroptosis in trigeminal neuralgiaMizhen Qiu0Fei Zeng1Yi Yan2Mu Xu3Xuexue Zhang4Zhijian Wang5Yutong Wang6Daying Zhang7Shibiao Chen8The First Affiliated Hospital, Jiangxi Medical College, Nanchang UniversityThe First Affiliated Hospital, Jiangxi Medical College, Nanchang UniversityThe First Affiliated Hospital, Jiangxi Medical College, Nanchang UniversityThe First Affiliated Hospital, Jiangxi Medical College, Nanchang UniversityThe First Affiliated Hospital, Jiangxi Medical College, Nanchang UniversityThe First Affiliated Hospital, Jiangxi Medical College, Nanchang UniversityThe Affiliated Hospital of Jiangxi University of Traditional Chinese MedicineThe First Affiliated Hospital, Jiangxi Medical College, Nanchang UniversityThe First Affiliated Hospital, Jiangxi Medical College, Nanchang UniversityThe mechanism of pyroptosis in trigeminal neuralgia (TN) progression remains unclear. The discovery of tRNA-derived small RNAs (tsRNAs) in the mechanism of pyroptosis is a breakthrough. We aim to study whether tsRNA is involved in TN progression through pyroptosis. A TN rat model was constructed by chronic constriction injury of infraorbital nerve and then mechanical hyperalgesia was quantitated using a withdrawal threshold test. Mechanical hyperalgesia was significantly elevated by constriction injury. Immunofluorescence revealed that expression of GSDMD and NLRP3 were significantly enhanced in TN rats. The expression of caspase-1, IL-1β, and IL-18 was significantly boosted after TN induction. CoPP + H2O2 exposure induced cell injury and pyroptosis of rat Schwann RSC96 cells, but reversed by VX-765 (a caspase-1 inhibitor). A total of 87 differentially expressed tsRNAs (DEtsRNAs) were identified in TN rats. KEGG pathway enrichment showed that DEtsRNAs were mainly involved in pyroptosis-related pathways, such as MAPK, ErbB, and mTOR signaling pathways. RT-qPCR showed that pyroptosis-related DEtsRNAs of tsRNA003489, tsRNA001840, and tsRNA003424 were downregulated in TN. Network analysis indicated tsRNA003489 and tsRNA001840 interact with NLRP3, a key regulator of pyroptosis. tsRNA has potential to participate in TN progression by targeting pyroptosis-related genes, which provides a new perspective for understanding pathology of TN.http://dx.doi.org/10.1080/26895293.2024.2381679nlrp3pyroptosistrigeminal neuralgiatrna-derived small rnas |
| spellingShingle | Mizhen Qiu Fei Zeng Yi Yan Mu Xu Xuexue Zhang Zhijian Wang Yutong Wang Daying Zhang Shibiao Chen Small RNA sequencing highlights potential functions of tsRNAs in regulating pyroptosis in trigeminal neuralgia All Life nlrp3 pyroptosis trigeminal neuralgia trna-derived small rnas |
| title | Small RNA sequencing highlights potential functions of tsRNAs in regulating pyroptosis in trigeminal neuralgia |
| title_full | Small RNA sequencing highlights potential functions of tsRNAs in regulating pyroptosis in trigeminal neuralgia |
| title_fullStr | Small RNA sequencing highlights potential functions of tsRNAs in regulating pyroptosis in trigeminal neuralgia |
| title_full_unstemmed | Small RNA sequencing highlights potential functions of tsRNAs in regulating pyroptosis in trigeminal neuralgia |
| title_short | Small RNA sequencing highlights potential functions of tsRNAs in regulating pyroptosis in trigeminal neuralgia |
| title_sort | small rna sequencing highlights potential functions of tsrnas in regulating pyroptosis in trigeminal neuralgia |
| topic | nlrp3 pyroptosis trigeminal neuralgia trna-derived small rnas |
| url | http://dx.doi.org/10.1080/26895293.2024.2381679 |
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