Small RNA sequencing highlights potential functions of tsRNAs in regulating pyroptosis in trigeminal neuralgia
The mechanism of pyroptosis in trigeminal neuralgia (TN) progression remains unclear. The discovery of tRNA-derived small RNAs (tsRNAs) in the mechanism of pyroptosis is a breakthrough. We aim to study whether tsRNA is involved in TN progression through pyroptosis. A TN rat model was constructed by...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
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| Series: | All Life |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/26895293.2024.2381679 |
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| Summary: | The mechanism of pyroptosis in trigeminal neuralgia (TN) progression remains unclear. The discovery of tRNA-derived small RNAs (tsRNAs) in the mechanism of pyroptosis is a breakthrough. We aim to study whether tsRNA is involved in TN progression through pyroptosis. A TN rat model was constructed by chronic constriction injury of infraorbital nerve and then mechanical hyperalgesia was quantitated using a withdrawal threshold test. Mechanical hyperalgesia was significantly elevated by constriction injury. Immunofluorescence revealed that expression of GSDMD and NLRP3 were significantly enhanced in TN rats. The expression of caspase-1, IL-1β, and IL-18 was significantly boosted after TN induction. CoPP + H2O2 exposure induced cell injury and pyroptosis of rat Schwann RSC96 cells, but reversed by VX-765 (a caspase-1 inhibitor). A total of 87 differentially expressed tsRNAs (DEtsRNAs) were identified in TN rats. KEGG pathway enrichment showed that DEtsRNAs were mainly involved in pyroptosis-related pathways, such as MAPK, ErbB, and mTOR signaling pathways. RT-qPCR showed that pyroptosis-related DEtsRNAs of tsRNA003489, tsRNA001840, and tsRNA003424 were downregulated in TN. Network analysis indicated tsRNA003489 and tsRNA001840 interact with NLRP3, a key regulator of pyroptosis. tsRNA has potential to participate in TN progression by targeting pyroptosis-related genes, which provides a new perspective for understanding pathology of TN. |
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| ISSN: | 2689-5307 |