Influence of different formulations and process parameters during the preparation of drug-loaded PLGA microspheres evaluated by multivariate data analysis
The main objective of this study was to evaluate the influence of the formulation and process parameters on PLGA microparticles containing a practically insoluble model drug (ibuprofen) prepared by the o/w solvent evaporation method. Multivariate data analysis was used. The effects of altered stirri...
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| Format: | Article |
| Language: | English |
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Sciendo
2014-12-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2014-0032 |
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| author | Vysloužil Jakub Doležel Petr Kejdušová Martina Mašková Eliška Mašek Josef Lukáč Robert Košťál Vratislav Vetchý David Dvořáčková Kateřina |
| author_facet | Vysloužil Jakub Doležel Petr Kejdušová Martina Mašková Eliška Mašek Josef Lukáč Robert Košťál Vratislav Vetchý David Dvořáčková Kateřina |
| author_sort | Vysloužil Jakub |
| collection | DOAJ |
| description | The main objective of this study was to evaluate the influence of the formulation and process parameters on PLGA microparticles containing a practically insoluble model drug (ibuprofen) prepared by the o/w solvent evaporation method. Multivariate data analysis was used. The effects of altered stirring speed of a mechanical stirrer (600, 1000 rpm), emulsifier concentrations (PVA concentration 0.1 %, 1 %) and solvent selection (dichloromethane, ethyl acetate) on microparticle characteristics (encapsulation efficiency, drug loading, burst effect) were observed. It was found that with increased stirring speed, the PVA concentration or the use of ethyl acetate had a significantly negative effect on encapsulation efficiency. In addition, ethyl acetate had an adverse effect on the burst effect, while increased stirring speed had the opposite effect. Drug load was not affected by any particular variable, but rather by the interactions of evaluated variables. |
| format | Article |
| id | doaj-art-19acb2c34684428384b10dd63dde2969 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2014-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-19acb2c34684428384b10dd63dde29692025-02-02T11:27:18ZengSciendoActa Pharmaceutica1846-95582014-12-0164440341710.2478/acph-2014-0032acph-2014-0032Influence of different formulations and process parameters during the preparation of drug-loaded PLGA microspheres evaluated by multivariate data analysisVysloužil Jakub0Doležel Petr1Kejdušová Martina2Mašková Eliška3Mašek Josef4Lukáč Robert5Košťál Vratislav6Vetchý David7Dvořáčková Kateřina8Department of Pharmaceutics Faculty of Pharmacy University of Veterinary and Pharmaceutical Sciences, Brno 61242 Brno, Czech RepublicDepartment of Pharmaceutics Faculty of Pharmacy University of Veterinary and Pharmaceutical Sciences, Brno 61242 Brno, Czech RepublicDepartment of Pharmaceutics Faculty of Pharmacy University of Veterinary and Pharmaceutical Sciences, Brno 61242 Brno, Czech RepublicDepartment of Pharmaceutics Faculty of Pharmacy University of Veterinary and Pharmaceutical Sciences, Brno 61242 Brno, Czech RepublicDepartment of Toxicology Pharmacology and Immunotherapy Veterinary Research Institute 62100 Brno, Czech RepublicDepartment of Toxicology Pharmacology and Immunotherapy Veterinary Research Institute 62100 Brno, Czech RepublicTescan, 62300 Brno-Kohoutovice Czech RepublicDepartment of Pharmaceutics Faculty of Pharmacy University of Veterinary and Pharmaceutical Sciences, Brno 61242 Brno, Czech RepublicDepartment of Pharmaceutics Faculty of Pharmacy University of Veterinary and Pharmaceutical Sciences, Brno 61242 Brno, Czech RepublicThe main objective of this study was to evaluate the influence of the formulation and process parameters on PLGA microparticles containing a practically insoluble model drug (ibuprofen) prepared by the o/w solvent evaporation method. Multivariate data analysis was used. The effects of altered stirring speed of a mechanical stirrer (600, 1000 rpm), emulsifier concentrations (PVA concentration 0.1 %, 1 %) and solvent selection (dichloromethane, ethyl acetate) on microparticle characteristics (encapsulation efficiency, drug loading, burst effect) were observed. It was found that with increased stirring speed, the PVA concentration or the use of ethyl acetate had a significantly negative effect on encapsulation efficiency. In addition, ethyl acetate had an adverse effect on the burst effect, while increased stirring speed had the opposite effect. Drug load was not affected by any particular variable, but rather by the interactions of evaluated variables.https://doi.org/10.2478/acph-2014-0032solvent evaporationencapsulationplgacontrolled releaseburst effect |
| spellingShingle | Vysloužil Jakub Doležel Petr Kejdušová Martina Mašková Eliška Mašek Josef Lukáč Robert Košťál Vratislav Vetchý David Dvořáčková Kateřina Influence of different formulations and process parameters during the preparation of drug-loaded PLGA microspheres evaluated by multivariate data analysis Acta Pharmaceutica solvent evaporation encapsulation plga controlled release burst effect |
| title | Influence of different formulations and process parameters during the preparation of drug-loaded PLGA microspheres evaluated by multivariate data analysis |
| title_full | Influence of different formulations and process parameters during the preparation of drug-loaded PLGA microspheres evaluated by multivariate data analysis |
| title_fullStr | Influence of different formulations and process parameters during the preparation of drug-loaded PLGA microspheres evaluated by multivariate data analysis |
| title_full_unstemmed | Influence of different formulations and process parameters during the preparation of drug-loaded PLGA microspheres evaluated by multivariate data analysis |
| title_short | Influence of different formulations and process parameters during the preparation of drug-loaded PLGA microspheres evaluated by multivariate data analysis |
| title_sort | influence of different formulations and process parameters during the preparation of drug loaded plga microspheres evaluated by multivariate data analysis |
| topic | solvent evaporation encapsulation plga controlled release burst effect |
| url | https://doi.org/10.2478/acph-2014-0032 |
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