Antibacterial Potential of Secondary Metabolites from Indonesian Marine Bacterial Symbionts
Indonesian marine environments are known to house diverse organisms. However, the potential for bacteria from these environments as a source of antibacterial agents has not been widely studied. This study aims to explore the antibacterial potential of secondary metabolites produced by bacterial symb...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2020/8898631 |
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| author | Risa Nofiani Alexandra J. Weisberg Takeshi Tsunoda Ruqiah Ganda Putri Panjaitan Ridho Brilliantoro Jeff H. Chang Benjamin Philmus Taifo Mahmud |
| author_facet | Risa Nofiani Alexandra J. Weisberg Takeshi Tsunoda Ruqiah Ganda Putri Panjaitan Ridho Brilliantoro Jeff H. Chang Benjamin Philmus Taifo Mahmud |
| author_sort | Risa Nofiani |
| collection | DOAJ |
| description | Indonesian marine environments are known to house diverse organisms. However, the potential for bacteria from these environments as a source of antibacterial agents has not been widely studied. This study aims to explore the antibacterial potential of secondary metabolites produced by bacterial symbionts from sponges and corals collected in the Indonesian waters. Extracts of 12 bacterial isolates from sponges or corals were prepared by cultivating the bacteria under a number of different media conditions and using agar well diffusion assays to test for antibacterial activity. In addition, the morphology, physiology, and biochemical characteristics and 16S rRNA sequence of each isolate were used to determine their taxonomic classification. All tested bacterial isolates were able to produce secondary metabolites with various levels of antibacterial activity depending on medium composition and culture conditions. Two of the bacteria (RS3 and RC4) showed strong antibacterial activities against both Gram-negative and Gram-positive bacteria. A number of isolates (RS1, RS3, and RC2) were co-cultured with mycolic acid-containing bacteria, Mycobacterium smegmatis or Rhodococcus sp. However, no improvements in their antibacterial activity were observed. All of the 12 bacteria tested were identified as Streptomyces spp. LC-MS analysis of EtOAc extracts from the most active strains RS3 and RC4 revealed the presence of a number of dactinomycin analogues and potentially new secondary metabolites. Symbiotic Streptomyces spp. from sponges and corals of the Indonesian marine environments have great potential as a source of broad-spectrum antibacterial agents. |
| format | Article |
| id | doaj-art-1998701cdec04031a2f444eb64a6a25e |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-1998701cdec04031a2f444eb64a6a25e2025-02-03T06:43:24ZengWileyInternational Journal of Microbiology1687-918X1687-91982020-01-01202010.1155/2020/88986318898631Antibacterial Potential of Secondary Metabolites from Indonesian Marine Bacterial SymbiontsRisa Nofiani0Alexandra J. Weisberg1Takeshi Tsunoda2Ruqiah Ganda Putri Panjaitan3Ridho Brilliantoro4Jeff H. Chang5Benjamin Philmus6Taifo Mahmud7Department of Chemistry, University of Tanjungpura, Pontianak 78124, IndonesiaDepartment of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, USADepartment of Pharmaceutical Sciences, Oregon State University, Corvallis, OR 97331, USADepartment of Biological Education, University of Tanjungpura, Pontianak 78124, IndonesiaDepartment of Chemistry, University of Tanjungpura, Pontianak 78124, IndonesiaDepartment of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, USADepartment of Pharmaceutical Sciences, Oregon State University, Corvallis, OR 97331, USADepartment of Pharmaceutical Sciences, Oregon State University, Corvallis, OR 97331, USAIndonesian marine environments are known to house diverse organisms. However, the potential for bacteria from these environments as a source of antibacterial agents has not been widely studied. This study aims to explore the antibacterial potential of secondary metabolites produced by bacterial symbionts from sponges and corals collected in the Indonesian waters. Extracts of 12 bacterial isolates from sponges or corals were prepared by cultivating the bacteria under a number of different media conditions and using agar well diffusion assays to test for antibacterial activity. In addition, the morphology, physiology, and biochemical characteristics and 16S rRNA sequence of each isolate were used to determine their taxonomic classification. All tested bacterial isolates were able to produce secondary metabolites with various levels of antibacterial activity depending on medium composition and culture conditions. Two of the bacteria (RS3 and RC4) showed strong antibacterial activities against both Gram-negative and Gram-positive bacteria. A number of isolates (RS1, RS3, and RC2) were co-cultured with mycolic acid-containing bacteria, Mycobacterium smegmatis or Rhodococcus sp. However, no improvements in their antibacterial activity were observed. All of the 12 bacteria tested were identified as Streptomyces spp. LC-MS analysis of EtOAc extracts from the most active strains RS3 and RC4 revealed the presence of a number of dactinomycin analogues and potentially new secondary metabolites. Symbiotic Streptomyces spp. from sponges and corals of the Indonesian marine environments have great potential as a source of broad-spectrum antibacterial agents.http://dx.doi.org/10.1155/2020/8898631 |
| spellingShingle | Risa Nofiani Alexandra J. Weisberg Takeshi Tsunoda Ruqiah Ganda Putri Panjaitan Ridho Brilliantoro Jeff H. Chang Benjamin Philmus Taifo Mahmud Antibacterial Potential of Secondary Metabolites from Indonesian Marine Bacterial Symbionts International Journal of Microbiology |
| title | Antibacterial Potential of Secondary Metabolites from Indonesian Marine Bacterial Symbionts |
| title_full | Antibacterial Potential of Secondary Metabolites from Indonesian Marine Bacterial Symbionts |
| title_fullStr | Antibacterial Potential of Secondary Metabolites from Indonesian Marine Bacterial Symbionts |
| title_full_unstemmed | Antibacterial Potential of Secondary Metabolites from Indonesian Marine Bacterial Symbionts |
| title_short | Antibacterial Potential of Secondary Metabolites from Indonesian Marine Bacterial Symbionts |
| title_sort | antibacterial potential of secondary metabolites from indonesian marine bacterial symbionts |
| url | http://dx.doi.org/10.1155/2020/8898631 |
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