Major royal-jelly proteins intake modulates immune functions and gut microbiota in mice
In this study, we investigated the effects of major royal jelly proteins (MRJPs) on the estrogen, gut microbiota, and immunological responses in mice. Mice given 250 or 500 mg/kg, not 125 mg/kg of MRJPs, enhanced the proliferation of splenocytes in response to mitogens. The splenocytes and mesenteri...
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Tsinghua University Press
2024-01-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023001386 |
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| author | Hang Wu Shican Zhou Wenjuan Ning Xiao Wu Xiaoxiao Xu Zejin Liu Wenhua Liu Kun Liu Lirong Shen Junpeng Wang |
| author_facet | Hang Wu Shican Zhou Wenjuan Ning Xiao Wu Xiaoxiao Xu Zejin Liu Wenhua Liu Kun Liu Lirong Shen Junpeng Wang |
| author_sort | Hang Wu |
| collection | DOAJ |
| description | In this study, we investigated the effects of major royal jelly proteins (MRJPs) on the estrogen, gut microbiota, and immunological responses in mice. Mice given 250 or 500 mg/kg, not 125 mg/kg of MRJPs, enhanced the proliferation of splenocytes in response to mitogens. The splenocytes and mesenteric lymphocytes activated by T-cell mitogens (ConA and anti-CD3/CD28 antibodies) released high levels of IL-2 but low levels of IFN-γ and IL-17A. The release of IL-4 was unaffected by MRJPs. Additionally, splenocytes and mesenteric lymphocytes activated by LPS were prevented by MRJPs at the same dose as that required for producing IL-1β and IL-6, two pro-inflammatory cytokines. The production of IL-1β, IL-6, and IFN-γ was negatively associated with estrogen levels, which were higher in the MRJP-treated animals than in the control group. Analysis of the gut microbiota revealed that feeding mice 250 mg/kg of MRJPs maintained the stability of the natural intestinal microflora of mice. Additionally, the LEfSe analysis identified biomarkers in the MRJP-treated mice, including Prevotella, Bacillales, Enterobacteriales, Gammaproteobacteria, Candidatus_Arthromitus, and Shigella. Our results showed that MRJPs are important components of royal jelly that modulate host immunity and hormone levels and help maintain gut microbiota stability. |
| format | Article |
| id | doaj-art-196d5efef251403c829435eedab06154 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-196d5efef251403c829435eedab061542025-02-03T04:09:17ZengTsinghua University PressFood Science and Human Wellness2213-45302024-01-01131444453Major royal-jelly proteins intake modulates immune functions and gut microbiota in miceHang Wu0Shican Zhou1Wenjuan Ning2Xiao Wu3Xiaoxiao Xu4Zejin Liu5Wenhua Liu6Kun Liu7Lirong Shen8Junpeng Wang9Infection and Immunity Institute and Translational Medical Center of Huaihe Hospital, Henan University, Kaifeng 475000, ChinaInfection and Immunity Institute and Translational Medical Center of Huaihe Hospital, Henan University, Kaifeng 475000, ChinaInfection and Immunity Institute and Translational Medical Center of Huaihe Hospital, Henan University, Kaifeng 475000, ChinaInfection and Immunity Institute and Translational Medical Center of Huaihe Hospital, Henan University, Kaifeng 475000, ChinaInfection and Immunity Institute and Translational Medical Center of Huaihe Hospital, Henan University, Kaifeng 475000, ChinaInfection and Immunity Institute and Translational Medical Center of Huaihe Hospital, Henan University, Kaifeng 475000, ChinaInfection and Immunity Institute and Translational Medical Center of Huaihe Hospital, Henan University, Kaifeng 475000, ChinaCollege of Biology Science and Engineering, Hebei University of Economics and Business, Shijiazhuang 050061, ChinaCollege of Biosystems Engineering and Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, ChinaInfection and Immunity Institute and Translational Medical Center of Huaihe Hospital, Henan University, Kaifeng 475000, China; Corresponding author at: 115 Ximen Street, Institute of Infection and Immunity and Translational Medical Center of Huaihe Hospital, Henan University, Kaifeng 475000, China.In this study, we investigated the effects of major royal jelly proteins (MRJPs) on the estrogen, gut microbiota, and immunological responses in mice. Mice given 250 or 500 mg/kg, not 125 mg/kg of MRJPs, enhanced the proliferation of splenocytes in response to mitogens. The splenocytes and mesenteric lymphocytes activated by T-cell mitogens (ConA and anti-CD3/CD28 antibodies) released high levels of IL-2 but low levels of IFN-γ and IL-17A. The release of IL-4 was unaffected by MRJPs. Additionally, splenocytes and mesenteric lymphocytes activated by LPS were prevented by MRJPs at the same dose as that required for producing IL-1β and IL-6, two pro-inflammatory cytokines. The production of IL-1β, IL-6, and IFN-γ was negatively associated with estrogen levels, which were higher in the MRJP-treated animals than in the control group. Analysis of the gut microbiota revealed that feeding mice 250 mg/kg of MRJPs maintained the stability of the natural intestinal microflora of mice. Additionally, the LEfSe analysis identified biomarkers in the MRJP-treated mice, including Prevotella, Bacillales, Enterobacteriales, Gammaproteobacteria, Candidatus_Arthromitus, and Shigella. Our results showed that MRJPs are important components of royal jelly that modulate host immunity and hormone levels and help maintain gut microbiota stability.http://www.sciencedirect.com/science/article/pii/S2213453023001386Major royal-jelly proteinsImmunityEstrogenGut microbiotaCytokines |
| spellingShingle | Hang Wu Shican Zhou Wenjuan Ning Xiao Wu Xiaoxiao Xu Zejin Liu Wenhua Liu Kun Liu Lirong Shen Junpeng Wang Major royal-jelly proteins intake modulates immune functions and gut microbiota in mice Food Science and Human Wellness Major royal-jelly proteins Immunity Estrogen Gut microbiota Cytokines |
| title | Major royal-jelly proteins intake modulates immune functions and gut microbiota in mice |
| title_full | Major royal-jelly proteins intake modulates immune functions and gut microbiota in mice |
| title_fullStr | Major royal-jelly proteins intake modulates immune functions and gut microbiota in mice |
| title_full_unstemmed | Major royal-jelly proteins intake modulates immune functions and gut microbiota in mice |
| title_short | Major royal-jelly proteins intake modulates immune functions and gut microbiota in mice |
| title_sort | major royal jelly proteins intake modulates immune functions and gut microbiota in mice |
| topic | Major royal-jelly proteins Immunity Estrogen Gut microbiota Cytokines |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023001386 |
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