Ultrasound‐assisted minimally invasive technique for tissue collection from bovine mammary glands
Abstract Background and Aims The objective of this case report was to describe an ultrasound‐guided, minimally invasive method for longitudinal mammary gland tissue collection from the bovine species. Materials and Methods Biopsies were performed on 14 8‐week‐old calves and 113 10‐week‐old calves. A...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-11-01
|
| Series: | Veterinary Medicine and Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/vms3.70027 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832593397262581760 |
|---|---|
| author | Alysia L. Vang Waneska S. Frizzarini Haylee H. Webster Thiago O. Cunha Kathryn M. Nelson Joao R. R. Dorea Laura L. Hernandez |
| author_facet | Alysia L. Vang Waneska S. Frizzarini Haylee H. Webster Thiago O. Cunha Kathryn M. Nelson Joao R. R. Dorea Laura L. Hernandez |
| author_sort | Alysia L. Vang |
| collection | DOAJ |
| description | Abstract Background and Aims The objective of this case report was to describe an ultrasound‐guided, minimally invasive method for longitudinal mammary gland tissue collection from the bovine species. Materials and Methods Biopsies were performed on 14 8‐week‐old calves and 113 10‐week‐old calves. A subset of 36 animals had repeated mammary gland biopsies through the first lactation. Mammary gland biopsies were performed using a disposable biopsy punch. The technique was also performed on multiparous cows on other independent research trials. Results One‐hundred and thirteen animals healed from the 10‐week biopsies with no complications. Of the 36 animals that received repeated biopsies, one developed mastitis due to premature suture removal and one had recurring mastitis in all quarters. Thirty‐three animals underwent all biopsies during gestation. Thirty of the original 36 are currently in lactation and still undergoing repeated biopsies. The method has also been successfully replicated on multiparous cows in separate studies. Discussion and Conclusion The described technique is a safe, reliable method for cattle mammary gland biopsies beginning at eight weeks of age and can be utilized to obtain repeated tissue collection from individual animals. The technique is also straightforward to perform and utilizes simple tools while providing acceptable amounts of tissue for most applications, with low risk for infection and long‐term tissue damage. |
| format | Article |
| id | doaj-art-195afac6985f4ad88b9bd08a593a7312 |
| institution | Kabale University |
| issn | 2053-1095 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj-art-195afac6985f4ad88b9bd08a593a73122025-01-20T17:16:44ZengWileyVeterinary Medicine and Science2053-10952024-11-01106n/an/a10.1002/vms3.70027Ultrasound‐assisted minimally invasive technique for tissue collection from bovine mammary glandsAlysia L. Vang0Waneska S. Frizzarini1Haylee H. Webster2Thiago O. Cunha3Kathryn M. Nelson4Joao R. R. Dorea5Laura L. Hernandez6Department of Animal and Dairy Sciences University of Wisconsin—Madison Madison Wisconsin USADepartment of Animal and Dairy Sciences University of Wisconsin—Madison Madison Wisconsin USADepartment of Animal and Dairy Sciences University of Wisconsin—Madison Madison Wisconsin USADepartment of Animal and Dairy Sciences University of Wisconsin—Madison Madison Wisconsin USADepartment of Animal and Dairy Sciences University of Wisconsin—Madison Madison Wisconsin USADepartment of Animal and Dairy Sciences University of Wisconsin—Madison Madison Wisconsin USADepartment of Animal and Dairy Sciences University of Wisconsin—Madison Madison Wisconsin USAAbstract Background and Aims The objective of this case report was to describe an ultrasound‐guided, minimally invasive method for longitudinal mammary gland tissue collection from the bovine species. Materials and Methods Biopsies were performed on 14 8‐week‐old calves and 113 10‐week‐old calves. A subset of 36 animals had repeated mammary gland biopsies through the first lactation. Mammary gland biopsies were performed using a disposable biopsy punch. The technique was also performed on multiparous cows on other independent research trials. Results One‐hundred and thirteen animals healed from the 10‐week biopsies with no complications. Of the 36 animals that received repeated biopsies, one developed mastitis due to premature suture removal and one had recurring mastitis in all quarters. Thirty‐three animals underwent all biopsies during gestation. Thirty of the original 36 are currently in lactation and still undergoing repeated biopsies. The method has also been successfully replicated on multiparous cows in separate studies. Discussion and Conclusion The described technique is a safe, reliable method for cattle mammary gland biopsies beginning at eight weeks of age and can be utilized to obtain repeated tissue collection from individual animals. The technique is also straightforward to perform and utilizes simple tools while providing acceptable amounts of tissue for most applications, with low risk for infection and long‐term tissue damage.https://doi.org/10.1002/vms3.70027biopsybovinelongitudinalmammary gland |
| spellingShingle | Alysia L. Vang Waneska S. Frizzarini Haylee H. Webster Thiago O. Cunha Kathryn M. Nelson Joao R. R. Dorea Laura L. Hernandez Ultrasound‐assisted minimally invasive technique for tissue collection from bovine mammary glands Veterinary Medicine and Science biopsy bovine longitudinal mammary gland |
| title | Ultrasound‐assisted minimally invasive technique for tissue collection from bovine mammary glands |
| title_full | Ultrasound‐assisted minimally invasive technique for tissue collection from bovine mammary glands |
| title_fullStr | Ultrasound‐assisted minimally invasive technique for tissue collection from bovine mammary glands |
| title_full_unstemmed | Ultrasound‐assisted minimally invasive technique for tissue collection from bovine mammary glands |
| title_short | Ultrasound‐assisted minimally invasive technique for tissue collection from bovine mammary glands |
| title_sort | ultrasound assisted minimally invasive technique for tissue collection from bovine mammary glands |
| topic | biopsy bovine longitudinal mammary gland |
| url | https://doi.org/10.1002/vms3.70027 |
| work_keys_str_mv | AT alysialvang ultrasoundassistedminimallyinvasivetechniquefortissuecollectionfrombovinemammaryglands AT waneskasfrizzarini ultrasoundassistedminimallyinvasivetechniquefortissuecollectionfrombovinemammaryglands AT hayleehwebster ultrasoundassistedminimallyinvasivetechniquefortissuecollectionfrombovinemammaryglands AT thiagoocunha ultrasoundassistedminimallyinvasivetechniquefortissuecollectionfrombovinemammaryglands AT kathrynmnelson ultrasoundassistedminimallyinvasivetechniquefortissuecollectionfrombovinemammaryglands AT joaorrdorea ultrasoundassistedminimallyinvasivetechniquefortissuecollectionfrombovinemammaryglands AT lauralhernandez ultrasoundassistedminimallyinvasivetechniquefortissuecollectionfrombovinemammaryglands |