Effects of Storage Temperature and Time on Stability of Serum Tacrolimus and Cyclosporine A Levels in Whole Blood by LC-MS/MS
Tacrolimus and cyclosporine A are immunosuppressant drugs with narrow therapeutic windows. The aim of this study was to investigate the stability of tacrolimus and cyclosporin A levels in whole blood samples under different storage conditions. Whole blood samples were obtained from 15 patients recei...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/956389 |
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| author | İbrahim Kaplan Hatice Yüksel Osman Evliyaoğlu M. Kemal Basarali Gülten Toprak Leyla Çolpan Velat Şen |
| author_facet | İbrahim Kaplan Hatice Yüksel Osman Evliyaoğlu M. Kemal Basarali Gülten Toprak Leyla Çolpan Velat Şen |
| author_sort | İbrahim Kaplan |
| collection | DOAJ |
| description | Tacrolimus and cyclosporine A are immunosuppressant drugs with narrow therapeutic windows. The aim of this study was to investigate the stability of tacrolimus and cyclosporin A levels in whole blood samples under different storage conditions. Whole blood samples were obtained from 15 patients receiving tacrolimus and 15 patients receiving cyclosporine A. Samples were immediately analyzed and then stored at different conditions (room temperature (24°C−26°C) for 24 hours, +4°C for 24 and 48 hours, and −20°C for one month) and then analyzed again. For tacrolimus, there was a significant difference between samples analyzed immediately and those kept 24 hours at room temperature (P=0.005) (percent change 32.89%). However, there were no significant differences between the other groups. For cyclosporine A, there was a significant difference between samples analyzed immediately and those kept 24 hours (P=0.003) (percent change 19.47%) and 48 hours (P=0.002) (percent change 15.38%) at +4°C and those kept 24 hours at room temperature (P=0.011) (percent change 9.71%). Samples of tacrolimus should be analyzed immediately or stored at either +4°C or −20°C, while samples of cyclosporine A should be analyzed immediately or stored at −20°C. |
| format | Article |
| id | doaj-art-19164e917b944900bea1ba36cd832dc7 |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-19164e917b944900bea1ba36cd832dc72025-02-03T01:11:28ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792015-01-01201510.1155/2015/956389956389Effects of Storage Temperature and Time on Stability of Serum Tacrolimus and Cyclosporine A Levels in Whole Blood by LC-MS/MSİbrahim Kaplan0Hatice Yüksel1Osman Evliyaoğlu2M. Kemal Basarali3Gülten Toprak4Leyla Çolpan5Velat Şen6Department of Biochemistry, Faculty of Medicine, Dicle University, Diyarbakir, TurkeyDepartment of Biochemistry, Faculty of Medicine, Dicle University, Diyarbakir, TurkeyDepartment of Biochemistry, Okmeydani Hospital, Istanbul, TurkeyDepartment of Biochemistry, Faculty of Medicine, Dicle University, Diyarbakir, TurkeyDepartment of Biochemistry, Faculty of Medicine, Dicle University, Diyarbakir, TurkeyDepartment of Biochemistry, Faculty of Medicine, Dicle University, Diyarbakir, TurkeyDepartment of Pediatrics, Faculty of Medicine, Dicle University, Diyarbakir, TurkeyTacrolimus and cyclosporine A are immunosuppressant drugs with narrow therapeutic windows. The aim of this study was to investigate the stability of tacrolimus and cyclosporin A levels in whole blood samples under different storage conditions. Whole blood samples were obtained from 15 patients receiving tacrolimus and 15 patients receiving cyclosporine A. Samples were immediately analyzed and then stored at different conditions (room temperature (24°C−26°C) for 24 hours, +4°C for 24 and 48 hours, and −20°C for one month) and then analyzed again. For tacrolimus, there was a significant difference between samples analyzed immediately and those kept 24 hours at room temperature (P=0.005) (percent change 32.89%). However, there were no significant differences between the other groups. For cyclosporine A, there was a significant difference between samples analyzed immediately and those kept 24 hours (P=0.003) (percent change 19.47%) and 48 hours (P=0.002) (percent change 15.38%) at +4°C and those kept 24 hours at room temperature (P=0.011) (percent change 9.71%). Samples of tacrolimus should be analyzed immediately or stored at either +4°C or −20°C, while samples of cyclosporine A should be analyzed immediately or stored at −20°C.http://dx.doi.org/10.1155/2015/956389 |
| spellingShingle | İbrahim Kaplan Hatice Yüksel Osman Evliyaoğlu M. Kemal Basarali Gülten Toprak Leyla Çolpan Velat Şen Effects of Storage Temperature and Time on Stability of Serum Tacrolimus and Cyclosporine A Levels in Whole Blood by LC-MS/MS International Journal of Analytical Chemistry |
| title | Effects of Storage Temperature and Time on Stability of Serum Tacrolimus and Cyclosporine A Levels in Whole Blood by LC-MS/MS |
| title_full | Effects of Storage Temperature and Time on Stability of Serum Tacrolimus and Cyclosporine A Levels in Whole Blood by LC-MS/MS |
| title_fullStr | Effects of Storage Temperature and Time on Stability of Serum Tacrolimus and Cyclosporine A Levels in Whole Blood by LC-MS/MS |
| title_full_unstemmed | Effects of Storage Temperature and Time on Stability of Serum Tacrolimus and Cyclosporine A Levels in Whole Blood by LC-MS/MS |
| title_short | Effects of Storage Temperature and Time on Stability of Serum Tacrolimus and Cyclosporine A Levels in Whole Blood by LC-MS/MS |
| title_sort | effects of storage temperature and time on stability of serum tacrolimus and cyclosporine a levels in whole blood by lc ms ms |
| url | http://dx.doi.org/10.1155/2015/956389 |
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