Construction of Trans-4-hydroxy-L-proline-producing <i>Escherichia coli</i> and Optimization of Fermentation Conditions
In this study, we combined the citric acid cycle with the biosynthesis pathways of L-proline and L-hydroxyproline to construct a strain that produces L-hydroxyproline directly from glucose and other raw materials, without the addition of L-proline and α-ketoglutarate. The results showed that the lev...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
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| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/11/2/54 |
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| Summary: | In this study, we combined the citric acid cycle with the biosynthesis pathways of L-proline and L-hydroxyproline to construct a strain that produces L-hydroxyproline directly from glucose and other raw materials, without the addition of L-proline and α-ketoglutarate. The results showed that the level of L-hydroxyproline production was 550 mg/L. Through the optimization of one-way and orthogonal experiments, the optimal shake flask fermentation conditions were obtained, at which time the production of L-hydroxyproline reached 1800 mg/L, which was 3.3-fold higher. The glutamate permease gene <i>GltS</i> was added to the recombinant plasmid pRSFDuet1-p4h-proBA, and the recombinant plasmid obtained was transformed into <i>E. coli</i> T7E by Gibson seamless cloning to obtain the recombinant strain T7E/pRSFDuet1-p4h-GltS-proBA. Finally, by the addition of 30 mmol/L of sodium glutamate, the recombinant strain achieved a yield of L-hydroxyproline of 2150 mg/L, which was about 1.2-fold higher than the yield of L-hydroxyproline without the addition of sodium glutamate. |
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| ISSN: | 2311-5637 |