Biocatalysis in Transforming Biofuel Technologies

Recently, the importance of biocatalysis in bioenergy has been noted, with policymakers and regulatory authorities intervening at the technological level to establish more efficient, varied, and vast-scale exploitations of biocatalysis. These approaches leverage natural catalysts, primarily enzymes,...

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Main Authors: Gautam Kumar Meghwanshi, Swati Verma, Rajaram Choyal, Abhishek Vashishtha, Nhung Thi Trang Trinh, Rajender Kumar
Format: Article
Language:English
Published: IMR Press 2025-06-01
Series:Frontiers in Bioscience-Elite
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Online Access:https://www.imrpress.com/journal/FBE/17/2/10.31083/FBE37729
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author Gautam Kumar Meghwanshi
Swati Verma
Rajaram Choyal
Abhishek Vashishtha
Nhung Thi Trang Trinh
Rajender Kumar
author_facet Gautam Kumar Meghwanshi
Swati Verma
Rajaram Choyal
Abhishek Vashishtha
Nhung Thi Trang Trinh
Rajender Kumar
author_sort Gautam Kumar Meghwanshi
collection DOAJ
description Recently, the importance of biocatalysis in bioenergy has been noted, with policymakers and regulatory authorities intervening at the technological level to establish more efficient, varied, and vast-scale exploitations of biocatalysis. These approaches leverage natural catalysts, primarily enzymes, to facilitate the breakdown of larger organic compounds into simpler molecules, which can be further biochemically transformed into biofuels, such as ethanol, biodiesel, and biogas, using improved versions of metabolic enzymes. Advances in enzyme engineering have significantly enhanced the stability, specificity, and activity of key enzymes involved in biofuel synthesis, such as cellulases, oxidoreductases, xylanases, glucose isomerases, butanol dehydrogenase, acetoacetate decarboxylase, ferredoxin oxidoreductases, etc. Further, synthetic biological approaches have allowed the construction of microbial cell factories with restructured integrated biocatalytic pathways, capable of converting the raw biomass directly into biofuels. Despite these advancements, challenges remain, such as the cost of enzymes, their robustness, and the scalability of their production and biotransformation processes. Ongoing research is focused on overcoming these hurdles through innovative biocatalyst design, metabolic engineering, in silico modeling, and optimization. However, changes in government policies and reduced regulatory frameworks are expected to leverage biofuel production and competitiveness with fossil fuels and gradually replace them completely. This review highlights the recent advances in the field of biocatalysis related to the production of biofuels. This review also discusses the current challenges, sustainability, promotional initiatives performed at the government level, and future directions in the field of biofuels.
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spelling doaj-art-1902d21bd9bf44acae914b0ae7431aac2025-08-20T03:50:11ZengIMR PressFrontiers in Bioscience-Elite1945-04942025-06-011723772910.31083/FBE37729S1945-0494(25)00188-2Biocatalysis in Transforming Biofuel TechnologiesGautam Kumar Meghwanshi0Swati Verma1Rajaram Choyal2Abhishek Vashishtha3Nhung Thi Trang Trinh4Rajender Kumar5Department of Microbiology, M.G.S. University, 334004 Bikaner, IndiaDepartment of Industrial Biotechnology, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), KTH Royal Institute of Technology, 114 28 Stockholm, SwedenDepartment of Environmental Sciences, M.G.S. University, 334004 Bikaner, IndiaDepartment of Microbiology, M.G.S. University, 334004 Bikaner, IndiaFaculty of Biomedical Sciences, Phenikaa University, Yen Nghia, Ha Dong, 12116 Hanoi, VietnamDepartment of Chemistry, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, AlbaNova University Center, 106 91 Stockholm, SwedenRecently, the importance of biocatalysis in bioenergy has been noted, with policymakers and regulatory authorities intervening at the technological level to establish more efficient, varied, and vast-scale exploitations of biocatalysis. These approaches leverage natural catalysts, primarily enzymes, to facilitate the breakdown of larger organic compounds into simpler molecules, which can be further biochemically transformed into biofuels, such as ethanol, biodiesel, and biogas, using improved versions of metabolic enzymes. Advances in enzyme engineering have significantly enhanced the stability, specificity, and activity of key enzymes involved in biofuel synthesis, such as cellulases, oxidoreductases, xylanases, glucose isomerases, butanol dehydrogenase, acetoacetate decarboxylase, ferredoxin oxidoreductases, etc. Further, synthetic biological approaches have allowed the construction of microbial cell factories with restructured integrated biocatalytic pathways, capable of converting the raw biomass directly into biofuels. Despite these advancements, challenges remain, such as the cost of enzymes, their robustness, and the scalability of their production and biotransformation processes. Ongoing research is focused on overcoming these hurdles through innovative biocatalyst design, metabolic engineering, in silico modeling, and optimization. However, changes in government policies and reduced regulatory frameworks are expected to leverage biofuel production and competitiveness with fossil fuels and gradually replace them completely. This review highlights the recent advances in the field of biocatalysis related to the production of biofuels. This review also discusses the current challenges, sustainability, promotional initiatives performed at the government level, and future directions in the field of biofuels.https://www.imrpress.com/journal/FBE/17/2/10.31083/FBE37729biocatalysisbiofuelsbiohydrogenbiodieselbiogasbioethanol
spellingShingle Gautam Kumar Meghwanshi
Swati Verma
Rajaram Choyal
Abhishek Vashishtha
Nhung Thi Trang Trinh
Rajender Kumar
Biocatalysis in Transforming Biofuel Technologies
Frontiers in Bioscience-Elite
biocatalysis
biofuels
biohydrogen
biodiesel
biogas
bioethanol
title Biocatalysis in Transforming Biofuel Technologies
title_full Biocatalysis in Transforming Biofuel Technologies
title_fullStr Biocatalysis in Transforming Biofuel Technologies
title_full_unstemmed Biocatalysis in Transforming Biofuel Technologies
title_short Biocatalysis in Transforming Biofuel Technologies
title_sort biocatalysis in transforming biofuel technologies
topic biocatalysis
biofuels
biohydrogen
biodiesel
biogas
bioethanol
url https://www.imrpress.com/journal/FBE/17/2/10.31083/FBE37729
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