Direct immunofluorescence for the diagnosis of legionellosis
Culture and direct immunofluorescent microscopy (DFA) results for Legionella pneumophila were reviewed over a two-year period. In the first year, a positive result was defined as having at least one morphologically typical fluorescing organism. In the second year, a positive was defined as at least...
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| Format: | Article |
| Language: | English |
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Wiley
1993-01-01
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| Series: | Canadian Journal of Infectious Diseases |
| Online Access: | http://dx.doi.org/10.1155/1993/909761 |
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| _version_ | 1832559054319255552 |
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| author | David JM Haldane Ruth Peppard Robert K Sumarah |
| author_facet | David JM Haldane Ruth Peppard Robert K Sumarah |
| author_sort | David JM Haldane |
| collection | DOAJ |
| description | Culture and direct immunofluorescent microscopy (DFA) results for Legionella pneumophila were reviewed over a two-year period. In the first year, a positive result was defined as having at least one morphologically typical fluorescing organism. In the second year, a positive was defined as at least five typical fluorescing organisms. Despite these stricter criteria and other measures to reduce the possibility of reagent contamination, there was no statistically significant difference in the sensitivity or specificity of the DFA in the two years for sputa, deep specimens or overall. Of 37 sputum specimens from infected patients, 16 were positive on DFA. Thirty-two of 38 positive patients were detected by sputum culture. DFA can provide rapid diagnostic information but cannot be used to rule out the diagnosis. Sputum is a useful specimen for the initial laboratory investigation of patients with legionellosis. |
| format | Article |
| id | doaj-art-18d721cf29054815ad1d53fbbde76587 |
| institution | Kabale University |
| issn | 1180-2332 |
| language | English |
| publishDate | 1993-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases |
| spelling | doaj-art-18d721cf29054815ad1d53fbbde765872025-02-03T01:30:49ZengWileyCanadian Journal of Infectious Diseases1180-23321993-01-014210110410.1155/1993/909761Direct immunofluorescence for the diagnosis of legionellosisDavid JM Haldane0Ruth Peppard1Robert K Sumarah2Department of Microbiology, Victoria General Hospital, Halifax, Nova Scotia, CanadaDepartment of Microbiology, Victoria General Hospital, Halifax, Nova Scotia, CanadaDepartment of Microbiology, Victoria General Hospital, Halifax, Nova Scotia, CanadaCulture and direct immunofluorescent microscopy (DFA) results for Legionella pneumophila were reviewed over a two-year period. In the first year, a positive result was defined as having at least one morphologically typical fluorescing organism. In the second year, a positive was defined as at least five typical fluorescing organisms. Despite these stricter criteria and other measures to reduce the possibility of reagent contamination, there was no statistically significant difference in the sensitivity or specificity of the DFA in the two years for sputa, deep specimens or overall. Of 37 sputum specimens from infected patients, 16 were positive on DFA. Thirty-two of 38 positive patients were detected by sputum culture. DFA can provide rapid diagnostic information but cannot be used to rule out the diagnosis. Sputum is a useful specimen for the initial laboratory investigation of patients with legionellosis.http://dx.doi.org/10.1155/1993/909761 |
| spellingShingle | David JM Haldane Ruth Peppard Robert K Sumarah Direct immunofluorescence for the diagnosis of legionellosis Canadian Journal of Infectious Diseases |
| title | Direct immunofluorescence for the diagnosis of legionellosis |
| title_full | Direct immunofluorescence for the diagnosis of legionellosis |
| title_fullStr | Direct immunofluorescence for the diagnosis of legionellosis |
| title_full_unstemmed | Direct immunofluorescence for the diagnosis of legionellosis |
| title_short | Direct immunofluorescence for the diagnosis of legionellosis |
| title_sort | direct immunofluorescence for the diagnosis of legionellosis |
| url | http://dx.doi.org/10.1155/1993/909761 |
| work_keys_str_mv | AT davidjmhaldane directimmunofluorescenceforthediagnosisoflegionellosis AT ruthpeppard directimmunofluorescenceforthediagnosisoflegionellosis AT robertksumarah directimmunofluorescenceforthediagnosisoflegionellosis |