Comparative study on the weight loss and lipid metabolism by tea polyphenols in diet induced obese C57BL/6J pseudo germ free and conventionalized mice
The role of gut microbiota in terms of host health is becoming increasingly important. In this study, the comparative effects of tea polyphenols (TPs) on weight loss and lipid metabolism on conventionalized mice (CVZ) and pseudo germ-free (PGF) mice (treated with antibiotics) were investigated. Our...
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Tsinghua University Press
2022-05-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453021001555 |
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| author | Kashif Hussain Yingying Yang Jie Wang Hengjuan Bian Xi Lei Junjie Chen Qianying Li Li Wang Qingping Zhong Xiang Fang Yutao Wang Hong Wei Yigang Tong Zhenlin Liao |
| author_facet | Kashif Hussain Yingying Yang Jie Wang Hengjuan Bian Xi Lei Junjie Chen Qianying Li Li Wang Qingping Zhong Xiang Fang Yutao Wang Hong Wei Yigang Tong Zhenlin Liao |
| author_sort | Kashif Hussain |
| collection | DOAJ |
| description | The role of gut microbiota in terms of host health is becoming increasingly important. In this study, the comparative effects of tea polyphenols (TPs) on weight loss and lipid metabolism on conventionalized mice (CVZ) and pseudo germ-free (PGF) mice (treated with antibiotics) were investigated. Our findings revealed that high fat (HF) diet considerably increased the body weight, total fat and upsurge lipid indices in CVZ mice but PGF mice were not sensitive to the effect of HF diet as CVZ mice. After the dietary administration of TP, body weight, perirenal fat and epididymal fat, liver weight, glucose (GLU) level, total chloestrol (TC level), high density lipoprotein-cholesterol (HDL-C) level significantly lowered in PGF mice as compared to CVZ mice group. However, the area of fat cells and triglyceride (TG) level were significantly increased in PGF mice. In CVZ mice, TP intervention resulted in a considerable drop in the Firmicutes/Bacteroides ratio as compared to PGF mice. The intestinal flora of PGF mice was severely reduced after antibiotic treatment, while TP administration restored intestinal diversity; the abundance of Akkermansia and Lactobacillus increased, whereas the abundance of Enterobacteriaceae and Prevotella reduced. Overall, we can assume that PGF obese mice administered with TP have less anti-obesity effects compared to obese CVZ mice. |
| format | Article |
| id | doaj-art-18d182755815421da470c7b320af9f53 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2022-05-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-18d182755815421da470c7b320af9f532025-02-02T23:24:54ZengTsinghua University PressFood Science and Human Wellness2213-45302022-05-01113697710Comparative study on the weight loss and lipid metabolism by tea polyphenols in diet induced obese C57BL/6J pseudo germ free and conventionalized miceKashif Hussain0Yingying Yang1Jie Wang2Hengjuan Bian3Xi Lei4Junjie Chen5Qianying Li6Li Wang7Qingping Zhong8Xiang Fang9Yutao Wang10Hong Wei11Yigang Tong12Zhenlin Liao13College of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, China; Department of Food Science, University of Guelph, Guelph, Ontario N1G 2W1, CanadaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Life and Geographic Sciences, Key Laboratory of Biological Resources and Ecology of Pamirs Plateau in Xinjiang Uygur Autonomous Region, Kashi University, Kashi 844000, ChinaInstitute of Precision Medicine, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, ChinaCollege of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, China; College of Life and Geographic Sciences, Key Laboratory of Biological Resources and Ecology of Pamirs Plateau in Xinjiang Uygur Autonomous Region, Kashi University, Kashi 844000, China; Corresponding author at: College of Food Science, South China Agricultural University, Guangzhou 510642, China. Tel/fax: +86 186 8023 8136.The role of gut microbiota in terms of host health is becoming increasingly important. In this study, the comparative effects of tea polyphenols (TPs) on weight loss and lipid metabolism on conventionalized mice (CVZ) and pseudo germ-free (PGF) mice (treated with antibiotics) were investigated. Our findings revealed that high fat (HF) diet considerably increased the body weight, total fat and upsurge lipid indices in CVZ mice but PGF mice were not sensitive to the effect of HF diet as CVZ mice. After the dietary administration of TP, body weight, perirenal fat and epididymal fat, liver weight, glucose (GLU) level, total chloestrol (TC level), high density lipoprotein-cholesterol (HDL-C) level significantly lowered in PGF mice as compared to CVZ mice group. However, the area of fat cells and triglyceride (TG) level were significantly increased in PGF mice. In CVZ mice, TP intervention resulted in a considerable drop in the Firmicutes/Bacteroides ratio as compared to PGF mice. The intestinal flora of PGF mice was severely reduced after antibiotic treatment, while TP administration restored intestinal diversity; the abundance of Akkermansia and Lactobacillus increased, whereas the abundance of Enterobacteriaceae and Prevotella reduced. Overall, we can assume that PGF obese mice administered with TP have less anti-obesity effects compared to obese CVZ mice.http://www.sciencedirect.com/science/article/pii/S2213453021001555High fat dietPseudo germ-free miceTea polyphenolObesityLipid metabolism |
| spellingShingle | Kashif Hussain Yingying Yang Jie Wang Hengjuan Bian Xi Lei Junjie Chen Qianying Li Li Wang Qingping Zhong Xiang Fang Yutao Wang Hong Wei Yigang Tong Zhenlin Liao Comparative study on the weight loss and lipid metabolism by tea polyphenols in diet induced obese C57BL/6J pseudo germ free and conventionalized mice Food Science and Human Wellness High fat diet Pseudo germ-free mice Tea polyphenol Obesity Lipid metabolism |
| title | Comparative study on the weight loss and lipid metabolism by tea polyphenols in diet induced obese C57BL/6J pseudo germ free and conventionalized mice |
| title_full | Comparative study on the weight loss and lipid metabolism by tea polyphenols in diet induced obese C57BL/6J pseudo germ free and conventionalized mice |
| title_fullStr | Comparative study on the weight loss and lipid metabolism by tea polyphenols in diet induced obese C57BL/6J pseudo germ free and conventionalized mice |
| title_full_unstemmed | Comparative study on the weight loss and lipid metabolism by tea polyphenols in diet induced obese C57BL/6J pseudo germ free and conventionalized mice |
| title_short | Comparative study on the weight loss and lipid metabolism by tea polyphenols in diet induced obese C57BL/6J pseudo germ free and conventionalized mice |
| title_sort | comparative study on the weight loss and lipid metabolism by tea polyphenols in diet induced obese c57bl 6j pseudo germ free and conventionalized mice |
| topic | High fat diet Pseudo germ-free mice Tea polyphenol Obesity Lipid metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S2213453021001555 |
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