INVESTIGATION OF HYPOLIPIDEMIC EFFECT OF SESQUITERPENE Γ-LACTONE AHILLIN IN HEPATOMA TISSUE CULTURE (HTC) CELLS
Objective. Investigation of hypolipidemic effect of sesquiterpene γ-lactone ahillin in hepatoma tissue culture (HTC) cells.Material and methods. In this study we’ve evaluated the effect of γ-lactone sesquiterpene aсhillin and gemfibrozil (comparator drug) on the lipid content in the hepatoma tissue...
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Siberian State Medical University (Tomsk)
2014-10-01
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| Series: | Бюллетень сибирской медицины |
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| Online Access: | https://bulletin.ssmu.ru/jour/article/view/203 |
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| author | V. V. Ivanov A. V. Ratkin Yu. A. Pfarger O. A. Kaidash N. V. Ryazantseva S. M. Adekenov V. S. Chuchalin |
| author_facet | V. V. Ivanov A. V. Ratkin Yu. A. Pfarger O. A. Kaidash N. V. Ryazantseva S. M. Adekenov V. S. Chuchalin |
| author_sort | V. V. Ivanov |
| collection | DOAJ |
| description | Objective. Investigation of hypolipidemic effect of sesquiterpene γ-lactone ahillin in hepatoma tissue culture (HTC) cells.Material and methods. In this study we’ve evaluated the effect of γ-lactone sesquiterpene aсhillin and gemfibrozil (comparator drug) on the lipid content in the hepatoma tissue culture (HTC) cell which were incubated with a fat emulsion lipofundin by fluorescent method with vital dye Nile Redand staining the cells with the dye Oil Red O. The cell viability was investigated using the MTT-test and staining with Trypan blue.Results. Cultivation cells HTC with aсhillin and gemfibrozilat concentrations ranging from 0.5 to1.5 mM and from0.25 mM to0.5 mM, respectively, resulted in dose-dependent decrease of the fluorescence’s intensity Nile Red. It reflects a decrease in lipid content in the cells. At these concentrations the drugs didn’t have cytotoxic effect and the cell viability didn’t change compared to the control culture.An experimental hyperlipidemia in the hepatoma culture cells was induced by adding to the incubation medium a fat emulsion lipofundin at a final concentration 0.05%. The intensity of fluorescence Nile Red in the cells was increased 4 fold (p < 0.05). This result suggests the significant accumulation of lipids in the cell’s cytosol and confirmed by microscopy after staining neutral lipids with the dye Oil Red O. Under these conditions aсhillin and gemfibrozil reduced lipid content in cells and hadthe effect at concentrations of0.5 mM and0.25 mM respectively.Conclusion. In the lipofundin-mediated model of hyperlipidemia the sesquiterpene lactone aсhillin prevents the lipid accumulation in cells. It confirms by decrease of fluorescence Nile Red and reduction lipid drops which were stained with Oil Red O in cytosol. To establish the molecular targets of aсhillin’saction on lipid metabolism in cell culture HTC we need to investigate a gene expression of key enzymes of lipid metabolism. |
| format | Article |
| id | doaj-art-18bd0cf8741a4e3bb74956a0a861c97a |
| institution | Kabale University |
| issn | 1682-0363 1819-3684 |
| language | English |
| publishDate | 2014-10-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj-art-18bd0cf8741a4e3bb74956a0a861c97a2025-08-20T03:38:07ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842014-10-01135283510.20538/1682-0363-2014-5-28-35201INVESTIGATION OF HYPOLIPIDEMIC EFFECT OF SESQUITERPENE Γ-LACTONE AHILLIN IN HEPATOMA TISSUE CULTURE (HTC) CELLSV. V. Ivanov0A. V. Ratkin1Yu. A. Pfarger2O. A. Kaidash3N. V. Ryazantseva4S. M. Adekenov5V. S. Chuchalin6Siberian State Medical University, TomskSiberian State Medical University, TomskSiberian State Medical University, TomskSiberian State Medical University, TomskSiberian State Medical University, TomskInternational scientific-industrial holding “Phytochemistry”, KaragandaSiberian State Medical University, TomskObjective. Investigation of hypolipidemic effect of sesquiterpene γ-lactone ahillin in hepatoma tissue culture (HTC) cells.Material and methods. In this study we’ve evaluated the effect of γ-lactone sesquiterpene aсhillin and gemfibrozil (comparator drug) on the lipid content in the hepatoma tissue culture (HTC) cell which were incubated with a fat emulsion lipofundin by fluorescent method with vital dye Nile Redand staining the cells with the dye Oil Red O. The cell viability was investigated using the MTT-test and staining with Trypan blue.Results. Cultivation cells HTC with aсhillin and gemfibrozilat concentrations ranging from 0.5 to1.5 mM and from0.25 mM to0.5 mM, respectively, resulted in dose-dependent decrease of the fluorescence’s intensity Nile Red. It reflects a decrease in lipid content in the cells. At these concentrations the drugs didn’t have cytotoxic effect and the cell viability didn’t change compared to the control culture.An experimental hyperlipidemia in the hepatoma culture cells was induced by adding to the incubation medium a fat emulsion lipofundin at a final concentration 0.05%. The intensity of fluorescence Nile Red in the cells was increased 4 fold (p < 0.05). This result suggests the significant accumulation of lipids in the cell’s cytosol and confirmed by microscopy after staining neutral lipids with the dye Oil Red O. Under these conditions aсhillin and gemfibrozil reduced lipid content in cells and hadthe effect at concentrations of0.5 mM and0.25 mM respectively.Conclusion. In the lipofundin-mediated model of hyperlipidemia the sesquiterpene lactone aсhillin prevents the lipid accumulation in cells. It confirms by decrease of fluorescence Nile Red and reduction lipid drops which were stained with Oil Red O in cytosol. To establish the molecular targets of aсhillin’saction on lipid metabolism in cell culture HTC we need to investigate a gene expression of key enzymes of lipid metabolism.https://bulletin.ssmu.ru/jour/article/view/203sesquiterpene γ-lactone aсhillingemfibrozilhepatoma tissue culture (htc) cellshypolipidemic effect |
| spellingShingle | V. V. Ivanov A. V. Ratkin Yu. A. Pfarger O. A. Kaidash N. V. Ryazantseva S. M. Adekenov V. S. Chuchalin INVESTIGATION OF HYPOLIPIDEMIC EFFECT OF SESQUITERPENE Γ-LACTONE AHILLIN IN HEPATOMA TISSUE CULTURE (HTC) CELLS Бюллетень сибирской медицины sesquiterpene γ-lactone aсhillin gemfibrozil hepatoma tissue culture (htc) cells hypolipidemic effect |
| title | INVESTIGATION OF HYPOLIPIDEMIC EFFECT OF SESQUITERPENE Γ-LACTONE AHILLIN IN HEPATOMA TISSUE CULTURE (HTC) CELLS |
| title_full | INVESTIGATION OF HYPOLIPIDEMIC EFFECT OF SESQUITERPENE Γ-LACTONE AHILLIN IN HEPATOMA TISSUE CULTURE (HTC) CELLS |
| title_fullStr | INVESTIGATION OF HYPOLIPIDEMIC EFFECT OF SESQUITERPENE Γ-LACTONE AHILLIN IN HEPATOMA TISSUE CULTURE (HTC) CELLS |
| title_full_unstemmed | INVESTIGATION OF HYPOLIPIDEMIC EFFECT OF SESQUITERPENE Γ-LACTONE AHILLIN IN HEPATOMA TISSUE CULTURE (HTC) CELLS |
| title_short | INVESTIGATION OF HYPOLIPIDEMIC EFFECT OF SESQUITERPENE Γ-LACTONE AHILLIN IN HEPATOMA TISSUE CULTURE (HTC) CELLS |
| title_sort | investigation of hypolipidemic effect of sesquiterpene γ lactone ahillin in hepatoma tissue culture htc cells |
| topic | sesquiterpene γ-lactone aсhillin gemfibrozil hepatoma tissue culture (htc) cells hypolipidemic effect |
| url | https://bulletin.ssmu.ru/jour/article/view/203 |
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