Effects of Long-Term Dietary Administration of Kale ( Brassica oleracea L. var. acephala DC) Leaves on the Antioxidant Status and Blood Biochemical Markers in Rats
Kale ( Brassica oleracea L. var. acephala DC) is a leafy green vegetable which belongs to the Brassicaceae family, one of the most commonly cultivated and consumed edible plants. The aim of this study was to investigate the potential adverse effects and antioxidant properties of freeze-dried kale...
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Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
2022-08-01
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| Series: | Polish Journal of Food and Nutrition Sciences |
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| Online Access: | http://journal.pan.olsztyn.pl/Effects-of-Long-Term-Dietary-Administration-of-Kale-Brassica-oleracea-L-var-acephala,152434,0,2.html |
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| author | Katarzyna Papierska Ewa Ignatowicz Jadwiga Jodynis-Liebert Małgorzata Kujawska Róża Biegańska-Marecik |
| author_facet | Katarzyna Papierska Ewa Ignatowicz Jadwiga Jodynis-Liebert Małgorzata Kujawska Róża Biegańska-Marecik |
| author_sort | Katarzyna Papierska |
| collection | DOAJ |
| description | Kale ( Brassica oleracea L. var. acephala DC) is a leafy green vegetable which belongs to the Brassicaceae family, one of the most commonly cultivated and consumed edible plants. The aim of this study was to investigate the potential adverse effects and antioxidant properties of freeze-dried kale leaves in 90-day dietary experiment on Wistar rats. The kale leaf powder was added to a diet at levels: 10, 30, and 60 g/kg feed. The standard blood biochemical and hematological markers, antioxidant enzyme activities, level of lipid peroxidation, reduced glutathione content, and damage to DNA in the liver were measured. Antioxidant potential in serum was measured and expressed as Trolox equivalents antioxidant capacity (TEAC). The kale leaf phytochemicals, i.e., glucosinolates, phenolic acids and flavonols, were quantified. Major glucosinolates were glucoiberin (164.6 mg/100 g d.w.) and glucobrassicin (130.6 mg/100 g d.w.), whereas kaempferol (159.1 mg/100 g d.w.), quercetin (119.6 mg/100 g d.w.), and sinapic acid (73.8 mg/100 g d.w.) dominated among phenolics. The lowest dose of kale leaves augmented the activity of catalase by 34% in males and by 44% in females. The highest applied dose increased activities of glutathione reductase by 31%, superoxide dismutase by 27%, and glutathione S-transferase by 24% in males only. Kale administration did not affect the activities of glutathione peroxidase and paraoxonase 1, glutathione concentration, lipid peroxidation level, and the level of DNA damage in the liver and the whole blood leukocytes. TEAC increased upon all doses of kale leaves by 30–90% in males and by 40–90% in females. The diet with kale did not affect blood biochemical and hematological markers. Results confirm no adverse effects of dried kale leaves used in model rat’s diet up to 60 g/kg for 90 days. The changes in hepatic antioxidant enzymes and plasma TEAC suggest beneficial effect of kale leaves on the antioxidant status in rats. Interestingly, these changes were more pronounced in male versus female animals. |
| format | Article |
| id | doaj-art-18ab8e2e69f64c3ebd180487ca304be7 |
| institution | Kabale University |
| issn | 2083-6007 |
| language | English |
| publishDate | 2022-08-01 |
| publisher | Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences |
| record_format | Article |
| series | Polish Journal of Food and Nutrition Sciences |
| spelling | doaj-art-18ab8e2e69f64c3ebd180487ca304be72025-02-02T03:40:18ZengInstitute of Animal Reproduction and Food Research of the Polish Academy of SciencesPolish Journal of Food and Nutrition Sciences2083-60072022-08-0172323924710.31883/pjfns/152434152434Effects of Long-Term Dietary Administration of Kale ( Brassica oleracea L. var. acephala DC) Leaves on the Antioxidant Status and Blood Biochemical Markers in RatsKatarzyna Papierska0https://orcid.org/0000-0002-5301-7944Ewa Ignatowicz1https://orcid.org/0000-0002-8128-0408Jadwiga Jodynis-Liebert2https://orcid.org/0000-0002-5008-4877Małgorzata Kujawska3https://orcid.org/0000-0002-5306-9904Róża Biegańska-Marecik4https://orcid.org/0000-0002-3734-4806Department of Pharmaceutical Biochemistry, Poznan University of Medical Sciences, 4 Święcickiego Str., 60-781 Poznań, PolandDepartment of Pharmaceutical Biochemistry, Poznan University of Medical Sciences, 4 Święcickiego Str., 60-781 Poznań, PolandDepartment of Toxicology, Poznan University of Medical Sciences, 30 Dojazd Str., 60-631 Poznań, PolandDepartment of Toxicology, Poznan University of Medical Sciences, 30 Dojazd Str., 60-631 Poznań, PolandDepartment of Food Technology of Plant Origin, Poznan University of Life Sciences, 31 Wojska Polskiego Str., 60-624 Poznan, PolandKale ( Brassica oleracea L. var. acephala DC) is a leafy green vegetable which belongs to the Brassicaceae family, one of the most commonly cultivated and consumed edible plants. The aim of this study was to investigate the potential adverse effects and antioxidant properties of freeze-dried kale leaves in 90-day dietary experiment on Wistar rats. The kale leaf powder was added to a diet at levels: 10, 30, and 60 g/kg feed. The standard blood biochemical and hematological markers, antioxidant enzyme activities, level of lipid peroxidation, reduced glutathione content, and damage to DNA in the liver were measured. Antioxidant potential in serum was measured and expressed as Trolox equivalents antioxidant capacity (TEAC). The kale leaf phytochemicals, i.e., glucosinolates, phenolic acids and flavonols, were quantified. Major glucosinolates were glucoiberin (164.6 mg/100 g d.w.) and glucobrassicin (130.6 mg/100 g d.w.), whereas kaempferol (159.1 mg/100 g d.w.), quercetin (119.6 mg/100 g d.w.), and sinapic acid (73.8 mg/100 g d.w.) dominated among phenolics. The lowest dose of kale leaves augmented the activity of catalase by 34% in males and by 44% in females. The highest applied dose increased activities of glutathione reductase by 31%, superoxide dismutase by 27%, and glutathione S-transferase by 24% in males only. Kale administration did not affect the activities of glutathione peroxidase and paraoxonase 1, glutathione concentration, lipid peroxidation level, and the level of DNA damage in the liver and the whole blood leukocytes. TEAC increased upon all doses of kale leaves by 30–90% in males and by 40–90% in females. The diet with kale did not affect blood biochemical and hematological markers. Results confirm no adverse effects of dried kale leaves used in model rat’s diet up to 60 g/kg for 90 days. The changes in hepatic antioxidant enzymes and plasma TEAC suggest beneficial effect of kale leaves on the antioxidant status in rats. Interestingly, these changes were more pronounced in male versus female animals.http://journal.pan.olsztyn.pl/Effects-of-Long-Term-Dietary-Administration-of-Kale-Brassica-oleracea-L-var-acephala,152434,0,2.htmlantioxidant statusdietary antioxidantsfunctional foodkale |
| spellingShingle | Katarzyna Papierska Ewa Ignatowicz Jadwiga Jodynis-Liebert Małgorzata Kujawska Róża Biegańska-Marecik Effects of Long-Term Dietary Administration of Kale ( Brassica oleracea L. var. acephala DC) Leaves on the Antioxidant Status and Blood Biochemical Markers in Rats Polish Journal of Food and Nutrition Sciences antioxidant status dietary antioxidants functional food kale |
| title | Effects of Long-Term Dietary Administration of Kale ( Brassica oleracea L. var. acephala DC) Leaves on the Antioxidant Status and Blood Biochemical Markers in Rats |
| title_full | Effects of Long-Term Dietary Administration of Kale ( Brassica oleracea L. var. acephala DC) Leaves on the Antioxidant Status and Blood Biochemical Markers in Rats |
| title_fullStr | Effects of Long-Term Dietary Administration of Kale ( Brassica oleracea L. var. acephala DC) Leaves on the Antioxidant Status and Blood Biochemical Markers in Rats |
| title_full_unstemmed | Effects of Long-Term Dietary Administration of Kale ( Brassica oleracea L. var. acephala DC) Leaves on the Antioxidant Status and Blood Biochemical Markers in Rats |
| title_short | Effects of Long-Term Dietary Administration of Kale ( Brassica oleracea L. var. acephala DC) Leaves on the Antioxidant Status and Blood Biochemical Markers in Rats |
| title_sort | effects of long term dietary administration of kale brassica oleracea l var acephala dc leaves on the antioxidant status and blood biochemical markers in rats |
| topic | antioxidant status dietary antioxidants functional food kale |
| url | http://journal.pan.olsztyn.pl/Effects-of-Long-Term-Dietary-Administration-of-Kale-Brassica-oleracea-L-var-acephala,152434,0,2.html |
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