The effect of culture media and seeding densities on the human malignant testicular germ cell line (NT2/D1) growth pattern
Testicular cancer is a common malignancy in young males, comprising 5% of urological cancers and 1% of all male cancers. The NT2/D1 cell line, a pluripotent embryonal carcinoma, is valuable in cancer and developmental biology research due to its differentiation potential into neurons and glial cells...
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PAGEPress Publications
2025-02-01
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| Series: | Journal of Biological Research |
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| Online Access: | https://www.pagepressjournals.org/jbr/article/view/13148 |
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| author | Rawabi S. Altuwayjiri Ibtesam S. Almami |
| author_facet | Rawabi S. Altuwayjiri Ibtesam S. Almami |
| author_sort | Rawabi S. Altuwayjiri |
| collection | DOAJ |
| description | Testicular cancer is a common malignancy in young males, comprising 5% of urological cancers and 1% of all male cancers. The NT2/D1 cell line, a pluripotent embryonal carcinoma, is valuable in cancer and developmental biology research due to its differentiation potential into neurons and glial cells. Although Fetal Bovine Serum (FBS) is often used to promote cell growth, its limitations necessitate optimized culture conditions. Monitoring cell growth and health through methods such as cell counting, viability assays, growth curves, and microscopy is crucial for reliable research, particularly for evaluating specific cell characteristics before in vitro studies. The study compared the growth patterns of NT2/D1 cell lines under different culture conditions and seeding densities using trypan blue cell counting and MTT methods. The NT2/D1 cell lines cultured in heat-inactivated FBS (H.iFBS) and non-heat-inactivated FBS (Non-H.iFBS) exhibit distinct growth patterns, doubling times, and cell morphologies. Cell density and the condition of the culture medium are two key parameters influencing metabolic activities and enhancing variability. There is no preference between culturing cells in different FBS conditions yet it should be considered in experiment design. Thus systematic reporting of all cell culture parameters is essential to facilitate better comparisons of in vitro and in vivo experimental data.
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| format | Article |
| id | doaj-art-18a5d175b9d0403aaad7f61dcdfa5167 |
| institution | Kabale University |
| issn | 1826-8838 2284-0230 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Journal of Biological Research |
| spelling | doaj-art-18a5d175b9d0403aaad7f61dcdfa51672025-02-06T10:56:32ZengPAGEPress PublicationsJournal of Biological Research1826-88382284-02302025-02-0110.4081/jbr.2025.13148The effect of culture media and seeding densities on the human malignant testicular germ cell line (NT2/D1) growth patternRawabi S. Altuwayjiri0Ibtesam S. Almami1Department of Biology, College of Science, Qassim University, Buraydah, Al-QassimDepartment of Biology, College of Science, Qassim University, Buraydah, Al-QassimTesticular cancer is a common malignancy in young males, comprising 5% of urological cancers and 1% of all male cancers. The NT2/D1 cell line, a pluripotent embryonal carcinoma, is valuable in cancer and developmental biology research due to its differentiation potential into neurons and glial cells. Although Fetal Bovine Serum (FBS) is often used to promote cell growth, its limitations necessitate optimized culture conditions. Monitoring cell growth and health through methods such as cell counting, viability assays, growth curves, and microscopy is crucial for reliable research, particularly for evaluating specific cell characteristics before in vitro studies. The study compared the growth patterns of NT2/D1 cell lines under different culture conditions and seeding densities using trypan blue cell counting and MTT methods. The NT2/D1 cell lines cultured in heat-inactivated FBS (H.iFBS) and non-heat-inactivated FBS (Non-H.iFBS) exhibit distinct growth patterns, doubling times, and cell morphologies. Cell density and the condition of the culture medium are two key parameters influencing metabolic activities and enhancing variability. There is no preference between culturing cells in different FBS conditions yet it should be considered in experiment design. Thus systematic reporting of all cell culture parameters is essential to facilitate better comparisons of in vitro and in vivo experimental data. https://www.pagepressjournals.org/jbr/article/view/13148Growth curveFBShuman malignanttesticular germ cell lineseeding densitiescell culture |
| spellingShingle | Rawabi S. Altuwayjiri Ibtesam S. Almami The effect of culture media and seeding densities on the human malignant testicular germ cell line (NT2/D1) growth pattern Journal of Biological Research Growth curve FBS human malignant testicular germ cell line seeding densities cell culture |
| title | The effect of culture media and seeding densities on the human malignant testicular germ cell line (NT2/D1) growth pattern |
| title_full | The effect of culture media and seeding densities on the human malignant testicular germ cell line (NT2/D1) growth pattern |
| title_fullStr | The effect of culture media and seeding densities on the human malignant testicular germ cell line (NT2/D1) growth pattern |
| title_full_unstemmed | The effect of culture media and seeding densities on the human malignant testicular germ cell line (NT2/D1) growth pattern |
| title_short | The effect of culture media and seeding densities on the human malignant testicular germ cell line (NT2/D1) growth pattern |
| title_sort | effect of culture media and seeding densities on the human malignant testicular germ cell line nt2 d1 growth pattern |
| topic | Growth curve FBS human malignant testicular germ cell line seeding densities cell culture |
| url | https://www.pagepressjournals.org/jbr/article/view/13148 |
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