NIR‐II AIEgens nanosystem for fluorescence and chemiluminescence synergistic imaging‐guided precise resection in osteosarcoma surgery
Abstract Osteosarcoma (OS) is characterized by an unfavorable prognosis and high mortality rates, with the local recurrence attributed to residual lesions post‐surgery being a major reason for treatment failure. Precise and tumor‐specific resection guidance to minimize recurrence remains a significa...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-01-01
|
| Series: | Aggregate |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/agt2.658 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832592333350109184 |
|---|---|
| author | Ruotong Li Kaiyuan Liu Qian Hu Jiakang Shen Dongqing Zuo Hongsheng Wang Xingjun Zhu Wei Sun |
| author_facet | Ruotong Li Kaiyuan Liu Qian Hu Jiakang Shen Dongqing Zuo Hongsheng Wang Xingjun Zhu Wei Sun |
| author_sort | Ruotong Li |
| collection | DOAJ |
| description | Abstract Osteosarcoma (OS) is characterized by an unfavorable prognosis and high mortality rates, with the local recurrence attributed to residual lesions post‐surgery being a major reason for treatment failure. Precise and tumor‐specific resection guidance to minimize recurrence remains a significant challenge. In the present study, a nanosystem based on aggregation‐induced emission (AIE) molecules with emission in the second near‐infrared window is proposed for the synergistic fluorescence (FL) and chemiluminescence (CL) imaging‐guided surgical resection for the elimination of tumor foci. The designed AIE molecule, BBTD14, exhibits stable FL with a high quantum yield of up to 3.95%, which effectively matches the energy levels of CL high‐energy states, generating the longest emission wavelength of CL reported to date. Targeted tumor imaging‐guided surgery (IGS) is facilitated by FL and CL nanoprobes (FLNP and CLNP) constructed based on BBTD14. During OS surgery, the FLNP, with the stability of FL and a high targeting capability, was first intravenously used to guide the surgical removal of the main tumor. Subsequently, CLNP was locally incubated to facilitate rapid and accurate evaluation of residual tumors at the operative border. High signal‐to‐noise ratio CL imaging was achieved after spraying with hydrogen peroxide, thereby overcoming the limitations of intraoperative frozen sections. The proposed technique also significantly reduced the recurrence rates in OS mouse models and exhibited high marker specificity in ex vivo OS patient pathology samples, confirming its potential in clinical applications and providing a unique perspective for developing IGS. |
| format | Article |
| id | doaj-art-18a3e10ae3f647f4ae2ad8d1e5702fbb |
| institution | Kabale University |
| issn | 2692-4560 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Aggregate |
| spelling | doaj-art-18a3e10ae3f647f4ae2ad8d1e5702fbb2025-01-21T08:57:07ZengWileyAggregate2692-45602025-01-0161n/an/a10.1002/agt2.658NIR‐II AIEgens nanosystem for fluorescence and chemiluminescence synergistic imaging‐guided precise resection in osteosarcoma surgeryRuotong Li0Kaiyuan Liu1Qian Hu2Jiakang Shen3Dongqing Zuo4Hongsheng Wang5Xingjun Zhu6Wei Sun7Department of Bone Tumor Surgery Shanghai General Hospital School of Medicine Shanghai Jiao Tong University Shanghai P. R. ChinaDepartment of Bone Tumor Surgery Shanghai General Hospital School of Medicine Shanghai Jiao Tong University Shanghai P. R. ChinaSchool of Physical Science and Technology & State Key Laboratory of Advanced Medical Materials and Devices ShanghaiTech University Shanghai P. R. ChinaDepartment of Bone Tumor Surgery Shanghai General Hospital School of Medicine Shanghai Jiao Tong University Shanghai P. R. ChinaDepartment of Bone Tumor Surgery Shanghai General Hospital School of Medicine Shanghai Jiao Tong University Shanghai P. R. ChinaDepartment of Bone Tumor Surgery Shanghai General Hospital School of Medicine Shanghai Jiao Tong University Shanghai P. R. ChinaSchool of Physical Science and Technology & State Key Laboratory of Advanced Medical Materials and Devices ShanghaiTech University Shanghai P. R. ChinaDepartment of Bone Tumor Surgery Shanghai General Hospital School of Medicine Shanghai Jiao Tong University Shanghai P. R. ChinaAbstract Osteosarcoma (OS) is characterized by an unfavorable prognosis and high mortality rates, with the local recurrence attributed to residual lesions post‐surgery being a major reason for treatment failure. Precise and tumor‐specific resection guidance to minimize recurrence remains a significant challenge. In the present study, a nanosystem based on aggregation‐induced emission (AIE) molecules with emission in the second near‐infrared window is proposed for the synergistic fluorescence (FL) and chemiluminescence (CL) imaging‐guided surgical resection for the elimination of tumor foci. The designed AIE molecule, BBTD14, exhibits stable FL with a high quantum yield of up to 3.95%, which effectively matches the energy levels of CL high‐energy states, generating the longest emission wavelength of CL reported to date. Targeted tumor imaging‐guided surgery (IGS) is facilitated by FL and CL nanoprobes (FLNP and CLNP) constructed based on BBTD14. During OS surgery, the FLNP, with the stability of FL and a high targeting capability, was first intravenously used to guide the surgical removal of the main tumor. Subsequently, CLNP was locally incubated to facilitate rapid and accurate evaluation of residual tumors at the operative border. High signal‐to‐noise ratio CL imaging was achieved after spraying with hydrogen peroxide, thereby overcoming the limitations of intraoperative frozen sections. The proposed technique also significantly reduced the recurrence rates in OS mouse models and exhibited high marker specificity in ex vivo OS patient pathology samples, confirming its potential in clinical applications and providing a unique perspective for developing IGS.https://doi.org/10.1002/agt2.658aggregation‐induced emissionchemiluminescencefluorescence‐guided surgeryosteosarcomasecond near‐infrared window |
| spellingShingle | Ruotong Li Kaiyuan Liu Qian Hu Jiakang Shen Dongqing Zuo Hongsheng Wang Xingjun Zhu Wei Sun NIR‐II AIEgens nanosystem for fluorescence and chemiluminescence synergistic imaging‐guided precise resection in osteosarcoma surgery Aggregate aggregation‐induced emission chemiluminescence fluorescence‐guided surgery osteosarcoma second near‐infrared window |
| title | NIR‐II AIEgens nanosystem for fluorescence and chemiluminescence synergistic imaging‐guided precise resection in osteosarcoma surgery |
| title_full | NIR‐II AIEgens nanosystem for fluorescence and chemiluminescence synergistic imaging‐guided precise resection in osteosarcoma surgery |
| title_fullStr | NIR‐II AIEgens nanosystem for fluorescence and chemiluminescence synergistic imaging‐guided precise resection in osteosarcoma surgery |
| title_full_unstemmed | NIR‐II AIEgens nanosystem for fluorescence and chemiluminescence synergistic imaging‐guided precise resection in osteosarcoma surgery |
| title_short | NIR‐II AIEgens nanosystem for fluorescence and chemiluminescence synergistic imaging‐guided precise resection in osteosarcoma surgery |
| title_sort | nir ii aiegens nanosystem for fluorescence and chemiluminescence synergistic imaging guided precise resection in osteosarcoma surgery |
| topic | aggregation‐induced emission chemiluminescence fluorescence‐guided surgery osteosarcoma second near‐infrared window |
| url | https://doi.org/10.1002/agt2.658 |
| work_keys_str_mv | AT ruotongli niriiaiegensnanosystemforfluorescenceandchemiluminescencesynergisticimagingguidedpreciseresectioninosteosarcomasurgery AT kaiyuanliu niriiaiegensnanosystemforfluorescenceandchemiluminescencesynergisticimagingguidedpreciseresectioninosteosarcomasurgery AT qianhu niriiaiegensnanosystemforfluorescenceandchemiluminescencesynergisticimagingguidedpreciseresectioninosteosarcomasurgery AT jiakangshen niriiaiegensnanosystemforfluorescenceandchemiluminescencesynergisticimagingguidedpreciseresectioninosteosarcomasurgery AT dongqingzuo niriiaiegensnanosystemforfluorescenceandchemiluminescencesynergisticimagingguidedpreciseresectioninosteosarcomasurgery AT hongshengwang niriiaiegensnanosystemforfluorescenceandchemiluminescencesynergisticimagingguidedpreciseresectioninosteosarcomasurgery AT xingjunzhu niriiaiegensnanosystemforfluorescenceandchemiluminescencesynergisticimagingguidedpreciseresectioninosteosarcomasurgery AT weisun niriiaiegensnanosystemforfluorescenceandchemiluminescencesynergisticimagingguidedpreciseresectioninosteosarcomasurgery |