EGF-R is Expressed and AP-1 and NF-κ:B Are Activated in Stromal Myofibroblasts Surrounding Colon Adenocarcinomas Paralleling Expression of COX-2 and VEGF
Background: COX-2 and VEGF are important triggers of colon cancer growth, metastasis and angiogenesis. Cox-2 promoter contains transcriptional regulatory elements for AP-1 and NF-κ:B transcription factors whilst vegf is a known AP-1 downstream target gene. We investigated whether stromal myofibrobla...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2007-01-01
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| Series: | Cellular Oncology |
| Online Access: | http://dx.doi.org/10.1155/2007/831416 |
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| _version_ | 1832554891689590784 |
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| author | Panagiotis A. Konstantinopoulos Gerasimos P. Vandoros Michalis V. Karamouzis Maria Gkermpesi Georgia Sotiropoulou-Bonikou Athanasios G. Papavassiliou |
| author_facet | Panagiotis A. Konstantinopoulos Gerasimos P. Vandoros Michalis V. Karamouzis Maria Gkermpesi Georgia Sotiropoulou-Bonikou Athanasios G. Papavassiliou |
| author_sort | Panagiotis A. Konstantinopoulos |
| collection | DOAJ |
| description | Background: COX-2 and VEGF are important triggers of colon cancer growth, metastasis and angiogenesis. Cox-2 promoter contains transcriptional regulatory elements for AP-1 and NF-κ:B transcription factors whilst vegf is a known AP-1 downstream target gene. We investigated whether stromal myofibroblasts surrounding colon adenocarcinomas express COX-2 and VEGF and whether activation of AP-1 and NF-κ:B, as well as expression of EGF-R parallel expression of COX-2 and VEGF in these cells. Methods: Immunohistochemical methodology was performed on archival sections from 40 patients with colon adenocarcinomas. We evaluated c-FOS, p-c-JUN (phosphorylated c-JUN), p-Iκ:B-α (phosphorylated Iκ:B-α), EGF-R, COX-2, NF-κ:B and VEGF expression in stromal myofibroblasts surrounding colon adenocarcinomas. Double immunostaining with a-smooth muscle actin and each antibody was done to verify the expression of these molecules in stromal myofibroblasts. Results: VEGF, p-Iκ:B-α, NF-κ:B, c-FOS, p-c-JUN, EGF-R and COX-2 were expressed in stromal myofibroblasts surrounding colon adenocarcinomas in the majority of cases. EGF-R, p-Iκ:B-α, NF-κ:B, c-FOS and p-c-JUN correlated positively with COX-2 and VEGF expression. Conclusion: Stromal myofibroblasts surrounding colon adenocarcinomas are an important source of VEGF and COX-2 production, while AP-1 and NF-κ:B transcription factors are activated and EGF-R is expressed in these cells and associated with COX-2 and VEGF production. |
| format | Article |
| id | doaj-art-18873fa20ef8445daf0b69ff4dd4a4d0 |
| institution | Kabale University |
| issn | 1570-5870 1875-8606 |
| language | English |
| publishDate | 2007-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cellular Oncology |
| spelling | doaj-art-18873fa20ef8445daf0b69ff4dd4a4d02025-02-03T05:50:13ZengWileyCellular Oncology1570-58701875-86062007-01-0129647748210.1155/2007/831416EGF-R is Expressed and AP-1 and NF-κ:B Are Activated in Stromal Myofibroblasts Surrounding Colon Adenocarcinomas Paralleling Expression of COX-2 and VEGFPanagiotis A. Konstantinopoulos0Gerasimos P. Vandoros1Michalis V. Karamouzis2Maria Gkermpesi3Georgia Sotiropoulou-Bonikou4Athanasios G. Papavassiliou5Department of Biological Chemistry, Medical School, University of Athens, Athens, GreeceDepartment of Pathology, Aeghion General Hospital, Aeghion, GreeceDepartment of Biological Chemistry, Medical School, University of Athens, Athens, GreeceDepartment of Pathology, Aeghion General Hospital, Aeghion, GreeceDepartment of Anatomy and Histology–Embryology, School of Medicine, University of Patras, Patras, GreeceDepartment of Biological Chemistry, Medical School, University of Athens, Athens, GreeceBackground: COX-2 and VEGF are important triggers of colon cancer growth, metastasis and angiogenesis. Cox-2 promoter contains transcriptional regulatory elements for AP-1 and NF-κ:B transcription factors whilst vegf is a known AP-1 downstream target gene. We investigated whether stromal myofibroblasts surrounding colon adenocarcinomas express COX-2 and VEGF and whether activation of AP-1 and NF-κ:B, as well as expression of EGF-R parallel expression of COX-2 and VEGF in these cells. Methods: Immunohistochemical methodology was performed on archival sections from 40 patients with colon adenocarcinomas. We evaluated c-FOS, p-c-JUN (phosphorylated c-JUN), p-Iκ:B-α (phosphorylated Iκ:B-α), EGF-R, COX-2, NF-κ:B and VEGF expression in stromal myofibroblasts surrounding colon adenocarcinomas. Double immunostaining with a-smooth muscle actin and each antibody was done to verify the expression of these molecules in stromal myofibroblasts. Results: VEGF, p-Iκ:B-α, NF-κ:B, c-FOS, p-c-JUN, EGF-R and COX-2 were expressed in stromal myofibroblasts surrounding colon adenocarcinomas in the majority of cases. EGF-R, p-Iκ:B-α, NF-κ:B, c-FOS and p-c-JUN correlated positively with COX-2 and VEGF expression. Conclusion: Stromal myofibroblasts surrounding colon adenocarcinomas are an important source of VEGF and COX-2 production, while AP-1 and NF-κ:B transcription factors are activated and EGF-R is expressed in these cells and associated with COX-2 and VEGF production.http://dx.doi.org/10.1155/2007/831416 |
| spellingShingle | Panagiotis A. Konstantinopoulos Gerasimos P. Vandoros Michalis V. Karamouzis Maria Gkermpesi Georgia Sotiropoulou-Bonikou Athanasios G. Papavassiliou EGF-R is Expressed and AP-1 and NF-κ:B Are Activated in Stromal Myofibroblasts Surrounding Colon Adenocarcinomas Paralleling Expression of COX-2 and VEGF Cellular Oncology |
| title | EGF-R is Expressed and AP-1 and NF-κ:B Are Activated in Stromal Myofibroblasts Surrounding Colon Adenocarcinomas Paralleling Expression of COX-2 and VEGF |
| title_full | EGF-R is Expressed and AP-1 and NF-κ:B Are Activated in Stromal Myofibroblasts Surrounding Colon Adenocarcinomas Paralleling Expression of COX-2 and VEGF |
| title_fullStr | EGF-R is Expressed and AP-1 and NF-κ:B Are Activated in Stromal Myofibroblasts Surrounding Colon Adenocarcinomas Paralleling Expression of COX-2 and VEGF |
| title_full_unstemmed | EGF-R is Expressed and AP-1 and NF-κ:B Are Activated in Stromal Myofibroblasts Surrounding Colon Adenocarcinomas Paralleling Expression of COX-2 and VEGF |
| title_short | EGF-R is Expressed and AP-1 and NF-κ:B Are Activated in Stromal Myofibroblasts Surrounding Colon Adenocarcinomas Paralleling Expression of COX-2 and VEGF |
| title_sort | egf r is expressed and ap 1 and nf κ b are activated in stromal myofibroblasts surrounding colon adenocarcinomas paralleling expression of cox 2 and vegf |
| url | http://dx.doi.org/10.1155/2007/831416 |
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