Modulation of adiposity and adipocyte inflammation by methanol extracts of Alpinia calcarata leaf in high-fat-diet induced-obese mice: Involvement of COX-2 and PPAR-γ
Obesity is a worldwide problem linked to several lifestyle disorders like diabetes, hypertension, heart failure, dyslipidaemias, asthma, etc. Finding a cure for obesity and its consequences is essential. Alpinia calcarata, a plant from the Zingiberaceae family, has been reported for several medicina...
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Elsevier
2025-01-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844025003299 |
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| author | Imtiaz Ahmad Maruful Hasan Dipty Rani Bhowmik Rahima Begum Sourav Roy Md Monirul Islam Md Abdur Rahman Ripon Mohammad Salim Hossain |
| author_facet | Imtiaz Ahmad Maruful Hasan Dipty Rani Bhowmik Rahima Begum Sourav Roy Md Monirul Islam Md Abdur Rahman Ripon Mohammad Salim Hossain |
| author_sort | Imtiaz Ahmad |
| collection | DOAJ |
| description | Obesity is a worldwide problem linked to several lifestyle disorders like diabetes, hypertension, heart failure, dyslipidaemias, asthma, etc. Finding a cure for obesity and its consequences is essential. Alpinia calcarata, a plant from the Zingiberaceae family, has been reported for several medicinal properties. The current study aimed to check out the role of Alpinia calcarata leaf in decreasing adiposity and adipocyte inflammation in high-fat diet-induced obese mice and understand the molecular principles underlying this occurrence. An in-silico test was done with more abounded compounds of Alpinia calcarata with adiposity and inflammatory genes. Moreover, methanol extract of Alpinia calcarata leaves were utilized to confirm the in-silico data in-vivo. High-fat diet induced-obese mice were treated with the extract at 200mg/kg-body weight dose. Body weight, organ weight, fat accumulation, serum cholesterol, serum triglyceride level, and liver function test were monitored as function of obesity. Alteration in the expression of IL-6, COX-2, MCP-1, PPARγ, TNFα, and GLUT-4 at transcript level were also studied. Our in-vivo results indicated that the plant extracts significantly (p < 0.05) decreased weight and accumulation of abdominal fat which was followed by a considerable reduction in total cholesterol and triglyceride levels. In agreement with the in-silico data, the extract was capable to reduce the mRNA expression of IL-6, COX-2, MCP-1, PPARγ, TNFα, and GLUT-4 which were consistent with the biochemical evidence; demonstrating the extract's capacity to attenuate adiposity and adipocyte inflammation. Taking it all together, it is noteworthy to report this novel function of A. calcarata leaf in reducing adiposity and adipocyte inflammation. |
| format | Article |
| id | doaj-art-187d3ecb4e654b0e860f05debd20424b |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-187d3ecb4e654b0e860f05debd20424b2025-02-02T05:28:37ZengElsevierHeliyon2405-84402025-01-01112e41949Modulation of adiposity and adipocyte inflammation by methanol extracts of Alpinia calcarata leaf in high-fat-diet induced-obese mice: Involvement of COX-2 and PPAR-γImtiaz Ahmad0Maruful Hasan1Dipty Rani Bhowmik2Rahima Begum3Sourav Roy4Md Monirul Islam5Md Abdur Rahman Ripon6Mohammad Salim Hossain7Department of Pharmacy, Noakhali Science and Technology University, Noakhali, 3814, BangladeshDepartment of Pharmacy, Noakhali Science and Technology University, Noakhali, 3814, BangladeshDepartment of Pharmacy, Noakhali Science and Technology University, Noakhali, 3814, BangladeshDepartment of Pharmacy, Noakhali Science and Technology University, Noakhali, 3814, BangladeshDepartment of Pharmacy, Noakhali Science and Technology University, Noakhali, 3814, BangladeshDepartment of Pharmacy, Noakhali Science and Technology University, Noakhali, 3814, BangladeshDepartment of Pharmacy, Noakhali Science and Technology University, Noakhali, 3814, BangladeshDepartment of Pharmacy, Noakhali Science and Technology University, Noakhali, 3814, Bangladesh; Bangladesh Obesity Research Network (BORN), Noakhali, 3814, Bangladesh; Corresponding author. Department of Pharmacy, Noakhali Science and Technology University, Noakhali, 3814, Bangladesh.Obesity is a worldwide problem linked to several lifestyle disorders like diabetes, hypertension, heart failure, dyslipidaemias, asthma, etc. Finding a cure for obesity and its consequences is essential. Alpinia calcarata, a plant from the Zingiberaceae family, has been reported for several medicinal properties. The current study aimed to check out the role of Alpinia calcarata leaf in decreasing adiposity and adipocyte inflammation in high-fat diet-induced obese mice and understand the molecular principles underlying this occurrence. An in-silico test was done with more abounded compounds of Alpinia calcarata with adiposity and inflammatory genes. Moreover, methanol extract of Alpinia calcarata leaves were utilized to confirm the in-silico data in-vivo. High-fat diet induced-obese mice were treated with the extract at 200mg/kg-body weight dose. Body weight, organ weight, fat accumulation, serum cholesterol, serum triglyceride level, and liver function test were monitored as function of obesity. Alteration in the expression of IL-6, COX-2, MCP-1, PPARγ, TNFα, and GLUT-4 at transcript level were also studied. Our in-vivo results indicated that the plant extracts significantly (p < 0.05) decreased weight and accumulation of abdominal fat which was followed by a considerable reduction in total cholesterol and triglyceride levels. In agreement with the in-silico data, the extract was capable to reduce the mRNA expression of IL-6, COX-2, MCP-1, PPARγ, TNFα, and GLUT-4 which were consistent with the biochemical evidence; demonstrating the extract's capacity to attenuate adiposity and adipocyte inflammation. Taking it all together, it is noteworthy to report this novel function of A. calcarata leaf in reducing adiposity and adipocyte inflammation.http://www.sciencedirect.com/science/article/pii/S2405844025003299Alpinia calcarataInflammationObesityAdipogenesisCOX-2PPARγ |
| spellingShingle | Imtiaz Ahmad Maruful Hasan Dipty Rani Bhowmik Rahima Begum Sourav Roy Md Monirul Islam Md Abdur Rahman Ripon Mohammad Salim Hossain Modulation of adiposity and adipocyte inflammation by methanol extracts of Alpinia calcarata leaf in high-fat-diet induced-obese mice: Involvement of COX-2 and PPAR-γ Heliyon Alpinia calcarata Inflammation Obesity Adipogenesis COX-2 PPARγ |
| title | Modulation of adiposity and adipocyte inflammation by methanol extracts of Alpinia calcarata leaf in high-fat-diet induced-obese mice: Involvement of COX-2 and PPAR-γ |
| title_full | Modulation of adiposity and adipocyte inflammation by methanol extracts of Alpinia calcarata leaf in high-fat-diet induced-obese mice: Involvement of COX-2 and PPAR-γ |
| title_fullStr | Modulation of adiposity and adipocyte inflammation by methanol extracts of Alpinia calcarata leaf in high-fat-diet induced-obese mice: Involvement of COX-2 and PPAR-γ |
| title_full_unstemmed | Modulation of adiposity and adipocyte inflammation by methanol extracts of Alpinia calcarata leaf in high-fat-diet induced-obese mice: Involvement of COX-2 and PPAR-γ |
| title_short | Modulation of adiposity and adipocyte inflammation by methanol extracts of Alpinia calcarata leaf in high-fat-diet induced-obese mice: Involvement of COX-2 and PPAR-γ |
| title_sort | modulation of adiposity and adipocyte inflammation by methanol extracts of alpinia calcarata leaf in high fat diet induced obese mice involvement of cox 2 and ppar γ |
| topic | Alpinia calcarata Inflammation Obesity Adipogenesis COX-2 PPARγ |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025003299 |
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