Candidate vaccine construction against tick-born encephalitis based on hybrid recombinant flagG-protE-protein
The present work describes the construction of the gene encoding the recombinant protein flagGprotE, its synthesis, purification and study. The recombinant flagGprotE protein is a promising molecule for developing a candidate recombinant vaccine against tickborne encephalitis by the ability to bin...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2018-01-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/1278 |
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| author | P. A. Belavin D. A. Kunyk E. V. Protopopova V. B. Loktev E. V. Deineko |
| author_facet | P. A. Belavin D. A. Kunyk E. V. Protopopova V. B. Loktev E. V. Deineko |
| author_sort | P. A. Belavin |
| collection | DOAJ |
| description | The present work describes the construction of the gene encoding the recombinant protein flagGprotE, its synthesis, purification and study. The recombinant flagGprotE protein is a promising molecule for developing a candidate recombinant vaccine against tickborne encephalitis by the ability to bind to monoclonal antibodies (MCA) against native protein E of tickborne encephalitis virus. The antigenic determinants of two recombinant proteins were studied: protE and flagGprotE using a panel of 8 MCA. The recombinant protein protE comprises the tickborne encephalitis virus envelope protein and the flagGprotE recombinant protein has an additional flagG domain encoding flagellin G of Salmonella typhi. It was found that the MCA tested revealed epitopes on the recombinant protein protE. This indicates that the investigated recombinant protein has an antigenic structure similar to the antigenic structure of the native tickborne encephalitis virus protein E. In the study of the recombinant protein flagGprotE by the ability to bind a panel of 8 MCA, only five of them react with epitopes of the tested protein. MCA 4F6, 7F10, and 6B9 did not recognize the corresponding epitope in the recombinant flagGprotE protein, while in the recombinant protein protE, these epitopes were detected successfully. Our data indicate that the antigenic structure of recombinant protEprotein can be changed under the influence of the flagellin domain, which in turn can lead to the unavailability of some antigenic determinants. This fact must be taken into account when constructing recombinant molecules with antigenic properties. Nevertheless, the fundamentally important regions in the region of the fusion peptide and III domain are antigenically present on the surface of the recombinant protein. This should ensure the formation of neutralizing antibodies, and the presence of a complete amino acid sequence of protein E in the recombinant protein induces the formation of a Tcell immune response. The emergence of a new generation of vaccines against tickborne encephalitis with a higher level of safety and immunogenicity will improve the vaccine prevention of the population from tickborne encephalitis. |
| format | Article |
| id | doaj-art-185e957251d34311ad350f30c2475041 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-185e957251d34311ad350f30c24750412025-02-01T09:58:05ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592018-01-0121898699210.18699/VJ17.323712Candidate vaccine construction against tick-born encephalitis based on hybrid recombinant flagG-protE-proteinP. A. Belavin0D. A. Kunyk1E. V. Protopopova2V. B. Loktev3E. V. Deineko4Institute of Cytology and Genetics SB RAS.Institute of Cytology and Genetics SB RAS.State Research Center of Virology and Biotechnology “Vector”.Institute of Cytology and Genetics SB RAS; State Research Center of Virology and Biotechnology “Vector”.Institute of Cytology and Genetics SB RAS.The present work describes the construction of the gene encoding the recombinant protein flagGprotE, its synthesis, purification and study. The recombinant flagGprotE protein is a promising molecule for developing a candidate recombinant vaccine against tickborne encephalitis by the ability to bind to monoclonal antibodies (MCA) against native protein E of tickborne encephalitis virus. The antigenic determinants of two recombinant proteins were studied: protE and flagGprotE using a panel of 8 MCA. The recombinant protein protE comprises the tickborne encephalitis virus envelope protein and the flagGprotE recombinant protein has an additional flagG domain encoding flagellin G of Salmonella typhi. It was found that the MCA tested revealed epitopes on the recombinant protein protE. This indicates that the investigated recombinant protein has an antigenic structure similar to the antigenic structure of the native tickborne encephalitis virus protein E. In the study of the recombinant protein flagGprotE by the ability to bind a panel of 8 MCA, only five of them react with epitopes of the tested protein. MCA 4F6, 7F10, and 6B9 did not recognize the corresponding epitope in the recombinant flagGprotE protein, while in the recombinant protein protE, these epitopes were detected successfully. Our data indicate that the antigenic structure of recombinant protEprotein can be changed under the influence of the flagellin domain, which in turn can lead to the unavailability of some antigenic determinants. This fact must be taken into account when constructing recombinant molecules with antigenic properties. Nevertheless, the fundamentally important regions in the region of the fusion peptide and III domain are antigenically present on the surface of the recombinant protein. This should ensure the formation of neutralizing antibodies, and the presence of a complete amino acid sequence of protein E in the recombinant protein induces the formation of a Tcell immune response. The emergence of a new generation of vaccines against tickborne encephalitis with a higher level of safety and immunogenicity will improve the vaccine prevention of the population from tickborne encephalitis.https://vavilov.elpub.ru/jour/article/view/1278flagellin genvelope proteinadjuvanttbeimmunogenicityvaccine |
| spellingShingle | P. A. Belavin D. A. Kunyk E. V. Protopopova V. B. Loktev E. V. Deineko Candidate vaccine construction against tick-born encephalitis based on hybrid recombinant flagG-protE-protein Вавиловский журнал генетики и селекции flagellin g envelope protein adjuvant tbe immunogenicity vaccine |
| title | Candidate vaccine construction against tick-born encephalitis based on hybrid recombinant flagG-protE-protein |
| title_full | Candidate vaccine construction against tick-born encephalitis based on hybrid recombinant flagG-protE-protein |
| title_fullStr | Candidate vaccine construction against tick-born encephalitis based on hybrid recombinant flagG-protE-protein |
| title_full_unstemmed | Candidate vaccine construction against tick-born encephalitis based on hybrid recombinant flagG-protE-protein |
| title_short | Candidate vaccine construction against tick-born encephalitis based on hybrid recombinant flagG-protE-protein |
| title_sort | candidate vaccine construction against tick born encephalitis based on hybrid recombinant flagg prote protein |
| topic | flagellin g envelope protein adjuvant tbe immunogenicity vaccine |
| url | https://vavilov.elpub.ru/jour/article/view/1278 |
| work_keys_str_mv | AT pabelavin candidatevaccineconstructionagainsttickbornencephalitisbasedonhybridrecombinantflaggproteprotein AT dakunyk candidatevaccineconstructionagainsttickbornencephalitisbasedonhybridrecombinantflaggproteprotein AT evprotopopova candidatevaccineconstructionagainsttickbornencephalitisbasedonhybridrecombinantflaggproteprotein AT vbloktev candidatevaccineconstructionagainsttickbornencephalitisbasedonhybridrecombinantflaggproteprotein AT evdeineko candidatevaccineconstructionagainsttickbornencephalitisbasedonhybridrecombinantflaggproteprotein |