Occupationally Acquired American Cutaneous Leishmaniasis
We report two occupationally acquired cases of American cutaneous leishmaniasis (ACL): one accidental laboratory autoinoculation by contaminated needlestick while handling an ACL lesion sample, and one acquired during field studies on bird biology. Polymerase chain reaction (PCR) assays of patient l...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Case Reports in Dermatological Medicine |
| Online Access: | http://dx.doi.org/10.1155/2012/279517 |
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| author | Maria Edileuza Felinto de Brito Maria Sandra Andrade Éricka Lima de Almeida Ângela Cristina Rapela Medeiros Roberto Pereira Werkhäuser Ana Isabele Freitas de Araújo Sinval Pinto Brandão-Filho Alzira Maria Paiva de Almeida Eduardo Henrique Gomes Rodrigues |
| author_facet | Maria Edileuza Felinto de Brito Maria Sandra Andrade Éricka Lima de Almeida Ângela Cristina Rapela Medeiros Roberto Pereira Werkhäuser Ana Isabele Freitas de Araújo Sinval Pinto Brandão-Filho Alzira Maria Paiva de Almeida Eduardo Henrique Gomes Rodrigues |
| author_sort | Maria Edileuza Felinto de Brito |
| collection | DOAJ |
| description | We report two occupationally acquired cases of American cutaneous leishmaniasis (ACL): one accidental laboratory autoinoculation by contaminated needlestick while handling an ACL lesion sample, and one acquired during field studies on bird biology. Polymerase chain reaction (PCR) assays of patient lesions were positive for Leishmania, subgenus Viannia. One isolate was obtained by culture (from patient 2 biopsy samples) and characterized as Leishmania (Viannia) naiffi through an indirect immunofluorescence assay (IFA) with species-specific monoclonal antibodies (mAbs) and by multilocus enzyme electrophoresis (MLEE). Patients were successfully treated with N-methyl-glucamine. These two cases highlight the potential risks of laboratory and field work and the need to comply with strict biosafety procedures in daily routines. The swab collection method, coupled with PCR detection, has greatly improved ACL laboratory diagnosis. |
| format | Article |
| id | doaj-art-184c007e3be14d159f9d4ac06142df59 |
| institution | Kabale University |
| issn | 2090-6463 2090-6471 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Case Reports in Dermatological Medicine |
| spelling | doaj-art-184c007e3be14d159f9d4ac06142df592025-02-03T01:11:31ZengWileyCase Reports in Dermatological Medicine2090-64632090-64712012-01-01201210.1155/2012/279517279517Occupationally Acquired American Cutaneous LeishmaniasisMaria Edileuza Felinto de Brito0Maria Sandra Andrade1Éricka Lima de Almeida2Ângela Cristina Rapela Medeiros3Roberto Pereira Werkhäuser4Ana Isabele Freitas de Araújo5Sinval Pinto Brandão-Filho6Alzira Maria Paiva de Almeida7Eduardo Henrique Gomes Rodrigues8Centro de Pesquisas Aggeu Magalhães, FIOCRUZ-PE, Campus da Universidade Federal de Pernambuco s/n, Cidade Universitária, 50.670-901 Recife, PE, BrazilDepartamentos de Enfermagem, Universidade de Pernambuco, Rua Arnóbio Marques 310, Santo Amaro, 50.100-130 Recife, PE, BrazilCentro de Pesquisas Aggeu Magalhães, FIOCRUZ-PE, Campus da Universidade Federal de Pernambuco s/n, Cidade Universitária, 50.670-901 Recife, PE, BrazilDepartamentos de Dermatologia, Universidade de Pernambuco, Rua Arnóbio Marques 310, Santo Amaro, 50.100-130 Recife, PE, BrazilCentro de Pesquisas Aggeu Magalhães, FIOCRUZ-PE, Campus da Universidade Federal de Pernambuco s/n, Cidade Universitária, 50.670-901 Recife, PE, BrazilCentro de Pesquisas Aggeu Magalhães, FIOCRUZ-PE, Campus da Universidade Federal de Pernambuco s/n, Cidade Universitária, 50.670-901 Recife, PE, BrazilCentro de Pesquisas Aggeu Magalhães, FIOCRUZ-PE, Campus da Universidade Federal de Pernambuco s/n, Cidade Universitária, 50.670-901 Recife, PE, BrazilCentro de Pesquisas Aggeu Magalhães, FIOCRUZ-PE, Campus da Universidade Federal de Pernambuco s/n, Cidade Universitária, 50.670-901 Recife, PE, BrazilCentro de Pesquisas Aggeu Magalhães, FIOCRUZ-PE, Campus da Universidade Federal de Pernambuco s/n, Cidade Universitária, 50.670-901 Recife, PE, BrazilWe report two occupationally acquired cases of American cutaneous leishmaniasis (ACL): one accidental laboratory autoinoculation by contaminated needlestick while handling an ACL lesion sample, and one acquired during field studies on bird biology. Polymerase chain reaction (PCR) assays of patient lesions were positive for Leishmania, subgenus Viannia. One isolate was obtained by culture (from patient 2 biopsy samples) and characterized as Leishmania (Viannia) naiffi through an indirect immunofluorescence assay (IFA) with species-specific monoclonal antibodies (mAbs) and by multilocus enzyme electrophoresis (MLEE). Patients were successfully treated with N-methyl-glucamine. These two cases highlight the potential risks of laboratory and field work and the need to comply with strict biosafety procedures in daily routines. The swab collection method, coupled with PCR detection, has greatly improved ACL laboratory diagnosis.http://dx.doi.org/10.1155/2012/279517 |
| spellingShingle | Maria Edileuza Felinto de Brito Maria Sandra Andrade Éricka Lima de Almeida Ângela Cristina Rapela Medeiros Roberto Pereira Werkhäuser Ana Isabele Freitas de Araújo Sinval Pinto Brandão-Filho Alzira Maria Paiva de Almeida Eduardo Henrique Gomes Rodrigues Occupationally Acquired American Cutaneous Leishmaniasis Case Reports in Dermatological Medicine |
| title | Occupationally Acquired American Cutaneous Leishmaniasis |
| title_full | Occupationally Acquired American Cutaneous Leishmaniasis |
| title_fullStr | Occupationally Acquired American Cutaneous Leishmaniasis |
| title_full_unstemmed | Occupationally Acquired American Cutaneous Leishmaniasis |
| title_short | Occupationally Acquired American Cutaneous Leishmaniasis |
| title_sort | occupationally acquired american cutaneous leishmaniasis |
| url | http://dx.doi.org/10.1155/2012/279517 |
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