Different Transcriptional Profiles of Human Monocyte-Derived Dendritic Cells Infected with Distinct Strains of Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin
In order to analyze dendritic cells (DCs) activation following infection with different mycobacterial strains, we studied the expression profiles of 165 genes of human monocyte-derived DCs infected with H37Rv, a virulent Mycobacterium tuberculosis (MTB) laboratory strain, CMT97, a clinical MTB isola...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2011/741051 |
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| author | Nunzia Sanarico Alessia Colone Manuela Grassi Viviana Speranza Daniela Giovannini Antonio Ciaramella Vittorio Colizzi Francesca Mariani |
| author_facet | Nunzia Sanarico Alessia Colone Manuela Grassi Viviana Speranza Daniela Giovannini Antonio Ciaramella Vittorio Colizzi Francesca Mariani |
| author_sort | Nunzia Sanarico |
| collection | DOAJ |
| description | In order to analyze dendritic cells (DCs) activation following infection with different mycobacterial strains, we studied the expression profiles of 165 genes of human monocyte-derived DCs infected with H37Rv, a virulent Mycobacterium tuberculosis (MTB) laboratory strain, CMT97, a clinical MTB isolate, Mycobacterium bovis bacillus Calmette-Guérin (BCG), Aventis Pasteur, and BCG Japan, both employed as vaccine against tuberculosis. The analysis of the gene expression reveals that, despite a set of genes similarly modulated, DCs response resulted strain dependent. In particular, H37Rv significantly upregulated EBI3 expression compared with BCG Japan, while it was the only strain that failed to release a significant IL-10 amount. Of note, BCG Japan showed a marked increase in CCR7 and TNF-α expression regarding both MTB strains and it resulted the only strain failing in exponential intracellular growth. Our results suggest that DCs display the ability to elicit a tailored strain-specific immune response. |
| format | Article |
| id | doaj-art-181b8296a99a4ebb84973bd39d44aa83 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-181b8296a99a4ebb84973bd39d44aa832025-02-03T01:21:15ZengWileyClinical and Developmental Immunology1740-25221740-25302011-01-01201110.1155/2011/741051741051Different Transcriptional Profiles of Human Monocyte-Derived Dendritic Cells Infected with Distinct Strains of Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-GuérinNunzia Sanarico0Alessia Colone1Manuela Grassi2Viviana Speranza3Daniela Giovannini4Antonio Ciaramella5Vittorio Colizzi6Francesca Mariani7Institute of Cell Biology and Neurobiology, National Research Council, Via del Fosso del Cavaliere, 100, 00133 Rome, ItalyInstitute of Cell Biology and Neurobiology, National Research Council, Via del Fosso del Cavaliere, 100, 00133 Rome, ItalyInstitute of Cell Biology and Neurobiology, National Research Council, Via del Fosso del Cavaliere, 100, 00133 Rome, ItalyInstitute of Cell Biology and Neurobiology, National Research Council, Via del Fosso del Cavaliere, 100, 00133 Rome, ItalyInstitute of Cell Biology and Neurobiology, National Research Council, Via del Fosso del Cavaliere, 100, 00133 Rome, ItalyDepartment of Clinical and Behavioral Neurology, IRCCS Santa Lucia Foundation, Via Ardeatina 306, 00179 Rome, ItalyDepartment of Biology, University of Rome “Tor Vergata”, Via della Ricerca Scientifica, 00133 Rome, ItalyInstitute of Cell Biology and Neurobiology, National Research Council, Via del Fosso del Cavaliere, 100, 00133 Rome, ItalyIn order to analyze dendritic cells (DCs) activation following infection with different mycobacterial strains, we studied the expression profiles of 165 genes of human monocyte-derived DCs infected with H37Rv, a virulent Mycobacterium tuberculosis (MTB) laboratory strain, CMT97, a clinical MTB isolate, Mycobacterium bovis bacillus Calmette-Guérin (BCG), Aventis Pasteur, and BCG Japan, both employed as vaccine against tuberculosis. The analysis of the gene expression reveals that, despite a set of genes similarly modulated, DCs response resulted strain dependent. In particular, H37Rv significantly upregulated EBI3 expression compared with BCG Japan, while it was the only strain that failed to release a significant IL-10 amount. Of note, BCG Japan showed a marked increase in CCR7 and TNF-α expression regarding both MTB strains and it resulted the only strain failing in exponential intracellular growth. Our results suggest that DCs display the ability to elicit a tailored strain-specific immune response.http://dx.doi.org/10.1155/2011/741051 |
| spellingShingle | Nunzia Sanarico Alessia Colone Manuela Grassi Viviana Speranza Daniela Giovannini Antonio Ciaramella Vittorio Colizzi Francesca Mariani Different Transcriptional Profiles of Human Monocyte-Derived Dendritic Cells Infected with Distinct Strains of Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin Clinical and Developmental Immunology |
| title | Different Transcriptional Profiles of Human Monocyte-Derived Dendritic Cells Infected with Distinct Strains of Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin |
| title_full | Different Transcriptional Profiles of Human Monocyte-Derived Dendritic Cells Infected with Distinct Strains of Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin |
| title_fullStr | Different Transcriptional Profiles of Human Monocyte-Derived Dendritic Cells Infected with Distinct Strains of Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin |
| title_full_unstemmed | Different Transcriptional Profiles of Human Monocyte-Derived Dendritic Cells Infected with Distinct Strains of Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin |
| title_short | Different Transcriptional Profiles of Human Monocyte-Derived Dendritic Cells Infected with Distinct Strains of Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin |
| title_sort | different transcriptional profiles of human monocyte derived dendritic cells infected with distinct strains of mycobacterium tuberculosis and mycobacterium bovis bacillus calmette guerin |
| url | http://dx.doi.org/10.1155/2011/741051 |
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