Primordial Germ Cells Quality of Chicken Embryo from Post Cryopreservation Blood Samples
The optimal cellular reservoir for the conservation and propagation of endangered poultry is acknowledged to be primordial germ cells (PGCs). This study aimed to evaluate and compare the efficacy of cryopreserving PGCs derived from purified sources and blood samples. The experimental design encompas...
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Universitas Airlangga, Faculty of Veterinary Medicine
2024-10-01
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| Series: | Jurnal Medik Veteriner |
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| Online Access: | https://e-journal.unair.ac.id/JMV/article/view/55140 |
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| author | Nurul Azizah Tatan Kostaman Yuli Arif Tribudi Diana Andrianita Kusumaningrum Suyatno Suyatno Zultinur Muttaqin Eko Koswara Muhammad Gunawan |
| author_facet | Nurul Azizah Tatan Kostaman Yuli Arif Tribudi Diana Andrianita Kusumaningrum Suyatno Suyatno Zultinur Muttaqin Eko Koswara Muhammad Gunawan |
| author_sort | Nurul Azizah |
| collection | DOAJ |
| description | The optimal cellular reservoir for the conservation and propagation of endangered poultry is acknowledged to be primordial germ cells (PGCs). This study aimed to evaluate and compare the efficacy of cryopreserving PGCs derived from purified sources and blood samples. The experimental design encompassed two treatment groups, each iterated six replication. Blood samples that had been filled with 500 µL of phosphate-buffered saline without Ca2+ and Mg2+ (PBS) underwent centrifugation at 1200 rpm for six minutes. The supernatant was discarded and the pellet was mixed with 500 µL 10% FBS-PBS and was then subdivided for cryopreservation and PGCs purification. Cryopreservation included the controlled freezing of blood and purified PGCs samples in Nalgene cryogenic vials with the incorporation of banker cells. Following thawing, statistical analyses revealed no significant variance in the mean PGCs count between purified PGCs and blood samples (p > 0.05). The percentages of PGCs mortality and the recovery rate demonstrated statistical significance (p < 0.05). Further investigations indicated that cryopreserving blood samples significantly increased the recovery rate of PGCs while decreasing post-thaw mortality. Morphology of PGCs from blood cryopreservation showed similar morphology to PGCs in general. The potential application of cell-based cryo-banking will contribute to the preservation of poultry genetic diversity for sustainability and adaptation to future poultry demands. |
| format | Article |
| id | doaj-art-180722f8d16c4ed1b27cdec1b99fd401 |
| institution | Kabale University |
| issn | 2615-7497 2581-012X |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Universitas Airlangga, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Jurnal Medik Veteriner |
| spelling | doaj-art-180722f8d16c4ed1b27cdec1b99fd4012025-02-03T11:58:36ZengUniversitas Airlangga, Faculty of Veterinary MedicineJurnal Medik Veteriner2615-74972581-012X2024-10-017229229910.20473/jmv.vol7.iss2.2024.292-29953302Primordial Germ Cells Quality of Chicken Embryo from Post Cryopreservation Blood SamplesNurul Azizah0https://orcid.org/0000-0003-0076-3596Tatan Kostaman1https://orcid.org/0000-0003-1248-0614Yuli Arif Tribudi2https://orcid.org/0000-0002-7852-9452Diana Andrianita Kusumaningrum3https://orcid.org/0000-0003-2509-8625Suyatno Suyatno4https://orcid.org/0000-0002-7765-2221Zultinur Muttaqin5https://orcid.org/0000-0001-6859-5934Eko Koswara6https://orcid.org/0009-0007-0582-2619Muhammad Gunawan7https://orcid.org/0000-0002-7940-0028Research Center of Animal Husbandry, Research Organization for Agriculture and Food, National Research and Innovation Agency (BRIN), Cibinong Sciences Center, Cibinong, Bogor 16915, IndonesiaResearch Center of Animal Husbandry, Research Organization for Agriculture and Food, National Research and Innovation Agency (BRIN), Cibinong Sciences Center, Cibinong, Bogor 16915, IndonesiaDepartment of Animal Science, Faculty of Agriculture, Universitas Tanjungpura, Pontianak, West Kalimantan 78124, IndonesiaResearch Center of Animal Husbandry, Research Organization for Agriculture and Food, National Research and Innovation Agency (BRIN), Cibinong Sciences Center, Cibinong, Bogor 16915, IndonesiaResearch Center of Animal Husbandry, Research Organization for Agriculture and Food, National Research and Innovation Agency (BRIN), Cibinong Sciences Center, Cibinong, Bogor 16915, IndonesiaResearch Center of Animal Husbandry, Research Organization for Agriculture and Food, National Research and Innovation Agency (BRIN), Cibinong Sciences Center, Cibinong, Bogor 16915, IndonesiaResearch Center of Animal Husbandry, Research Organization for Agriculture and Food, National Research and Innovation Agency (BRIN), Cibinong Sciences Center, Cibinong, Bogor 16915, IndonesiaResearch Center for Applied Zoology, Research Organization for Life Sciences and Environment, National Research and Innovation Agency (BRIN), Cibinong Sciences Center, Cibinong, Bogor 16915, IndonesiaThe optimal cellular reservoir for the conservation and propagation of endangered poultry is acknowledged to be primordial germ cells (PGCs). This study aimed to evaluate and compare the efficacy of cryopreserving PGCs derived from purified sources and blood samples. The experimental design encompassed two treatment groups, each iterated six replication. Blood samples that had been filled with 500 µL of phosphate-buffered saline without Ca2+ and Mg2+ (PBS) underwent centrifugation at 1200 rpm for six minutes. The supernatant was discarded and the pellet was mixed with 500 µL 10% FBS-PBS and was then subdivided for cryopreservation and PGCs purification. Cryopreservation included the controlled freezing of blood and purified PGCs samples in Nalgene cryogenic vials with the incorporation of banker cells. Following thawing, statistical analyses revealed no significant variance in the mean PGCs count between purified PGCs and blood samples (p > 0.05). The percentages of PGCs mortality and the recovery rate demonstrated statistical significance (p < 0.05). Further investigations indicated that cryopreserving blood samples significantly increased the recovery rate of PGCs while decreasing post-thaw mortality. Morphology of PGCs from blood cryopreservation showed similar morphology to PGCs in general. The potential application of cell-based cryo-banking will contribute to the preservation of poultry genetic diversity for sustainability and adaptation to future poultry demands.https://e-journal.unair.ac.id/JMV/article/view/55140bloodcryopreservationendangered poultryprimordial germ cellspurified |
| spellingShingle | Nurul Azizah Tatan Kostaman Yuli Arif Tribudi Diana Andrianita Kusumaningrum Suyatno Suyatno Zultinur Muttaqin Eko Koswara Muhammad Gunawan Primordial Germ Cells Quality of Chicken Embryo from Post Cryopreservation Blood Samples Jurnal Medik Veteriner blood cryopreservation endangered poultry primordial germ cells purified |
| title | Primordial Germ Cells Quality of Chicken Embryo from Post Cryopreservation Blood Samples |
| title_full | Primordial Germ Cells Quality of Chicken Embryo from Post Cryopreservation Blood Samples |
| title_fullStr | Primordial Germ Cells Quality of Chicken Embryo from Post Cryopreservation Blood Samples |
| title_full_unstemmed | Primordial Germ Cells Quality of Chicken Embryo from Post Cryopreservation Blood Samples |
| title_short | Primordial Germ Cells Quality of Chicken Embryo from Post Cryopreservation Blood Samples |
| title_sort | primordial germ cells quality of chicken embryo from post cryopreservation blood samples |
| topic | blood cryopreservation endangered poultry primordial germ cells purified |
| url | https://e-journal.unair.ac.id/JMV/article/view/55140 |
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