Isolation, Characterization, and Identification of Biological Control Agent for Potato Soft Rot in Bangladesh
A total of 91 isolates of probable antagonistic bacteria of potato soft rot bacterium Erwinia carotovora subsp. carotovora (Ecc) were extracted from rhizospheres and endophytes of various crop plants, different soil varieties, and atmospheres in the potato farming areas of Bangladesh. Antibacterial...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/723293 |
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| author | M. M. Rahman M. E. Ali A. A. Khan A. M. Akanda Md. Kamal Uddin U. Hashim S. B. Abd Hamid |
| author_facet | M. M. Rahman M. E. Ali A. A. Khan A. M. Akanda Md. Kamal Uddin U. Hashim S. B. Abd Hamid |
| author_sort | M. M. Rahman |
| collection | DOAJ |
| description | A total of 91 isolates of probable antagonistic bacteria of potato soft rot bacterium Erwinia carotovora subsp. carotovora (Ecc) were extracted from rhizospheres and endophytes of various crop plants, different soil varieties, and atmospheres in the potato farming areas of Bangladesh. Antibacterial activity of the isolated probable antagonistic bacteria was tested in vitro against the previously identified most common and most virulent soft rot causing bacterial strain Ecc P-138. Only two isolates E-45 and E-65 significantly inhibited the in vitro growth of Ecc P-138. Physiological, biochemical, and carbon source utilization tests identified isolate E-65 as a member of the genus Bacillus and the isolate E-45 as Lactobacillus sp. The stronger antagonistic activity against Ecc P-138 was found in E-65 in vitro screening and storage potatoes. E-65 reduced the soft rot infection to 22-week storage potatoes of different varieties by 32.5–62.5% in model experiment, demonstrating its strong potential to be used as an effective biological control agent for the major pectolytic bacteria Ecc. The highest (62.5%) antagonistic effect of E-65 was observed in the Granola and the lowest (32.7%) of that was found in the Cardinal varieties of the Bangladeshi potatoes. The findings suggest that isolate E-65 could be exploited as a biocontrol agent for potato tubers. |
| format | Article |
| id | doaj-art-17f50e08ee954cf2af832c072c32673f |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-17f50e08ee954cf2af832c072c32673f2025-02-03T01:23:19ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/723293723293Isolation, Characterization, and Identification of Biological Control Agent for Potato Soft Rot in BangladeshM. M. Rahman0M. E. Ali1A. A. Khan2A. M. Akanda3Md. Kamal Uddin4U. Hashim5S. B. Abd Hamid6Department of Plant Pathology, Bangabadhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, BangladeshNanotechnology and Catalysis Research Center, Universiti Malaya, 50603 Kuala Lumpur, MalaysiaDepartment of Plant Pathology, Bangabadhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, BangladeshDepartment of Plant Pathology, Bangabadhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, BangladeshInstitute of Tropical Agriculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, MalaysiaInstitute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000 Kangar, MalaysiaNanotechnology and Catalysis Research Center, Universiti Malaya, 50603 Kuala Lumpur, MalaysiaA total of 91 isolates of probable antagonistic bacteria of potato soft rot bacterium Erwinia carotovora subsp. carotovora (Ecc) were extracted from rhizospheres and endophytes of various crop plants, different soil varieties, and atmospheres in the potato farming areas of Bangladesh. Antibacterial activity of the isolated probable antagonistic bacteria was tested in vitro against the previously identified most common and most virulent soft rot causing bacterial strain Ecc P-138. Only two isolates E-45 and E-65 significantly inhibited the in vitro growth of Ecc P-138. Physiological, biochemical, and carbon source utilization tests identified isolate E-65 as a member of the genus Bacillus and the isolate E-45 as Lactobacillus sp. The stronger antagonistic activity against Ecc P-138 was found in E-65 in vitro screening and storage potatoes. E-65 reduced the soft rot infection to 22-week storage potatoes of different varieties by 32.5–62.5% in model experiment, demonstrating its strong potential to be used as an effective biological control agent for the major pectolytic bacteria Ecc. The highest (62.5%) antagonistic effect of E-65 was observed in the Granola and the lowest (32.7%) of that was found in the Cardinal varieties of the Bangladeshi potatoes. The findings suggest that isolate E-65 could be exploited as a biocontrol agent for potato tubers.http://dx.doi.org/10.1100/2012/723293 |
| spellingShingle | M. M. Rahman M. E. Ali A. A. Khan A. M. Akanda Md. Kamal Uddin U. Hashim S. B. Abd Hamid Isolation, Characterization, and Identification of Biological Control Agent for Potato Soft Rot in Bangladesh The Scientific World Journal |
| title | Isolation, Characterization, and Identification of Biological Control Agent for Potato Soft Rot in Bangladesh |
| title_full | Isolation, Characterization, and Identification of Biological Control Agent for Potato Soft Rot in Bangladesh |
| title_fullStr | Isolation, Characterization, and Identification of Biological Control Agent for Potato Soft Rot in Bangladesh |
| title_full_unstemmed | Isolation, Characterization, and Identification of Biological Control Agent for Potato Soft Rot in Bangladesh |
| title_short | Isolation, Characterization, and Identification of Biological Control Agent for Potato Soft Rot in Bangladesh |
| title_sort | isolation characterization and identification of biological control agent for potato soft rot in bangladesh |
| url | http://dx.doi.org/10.1100/2012/723293 |
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