Identification of the Related Substances in Ampicillin Capsule by Rapid Resolution Liquid Chromatography Coupled with Electrospray Ionization Tandem Mass Spectrometry
Rapid Resolution Liquid Chromatography coupled with Electrospray Ionization Tandem Mass Spectrometry (RRLC-ESI-MSn) was used to separate and identify related substances in ampicillin capsule. The fragmentation behaviors of related substances were used to identify their chemical structures. Finally,...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2014/397492 |
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| author | Lei Zhang Xian Long Cheng Yang Liu Miao Liang Honghuan Dong Beiran Lv Wenning Yang Zhiqiang Luo Mingmin Tang |
| author_facet | Lei Zhang Xian Long Cheng Yang Liu Miao Liang Honghuan Dong Beiran Lv Wenning Yang Zhiqiang Luo Mingmin Tang |
| author_sort | Lei Zhang |
| collection | DOAJ |
| description | Rapid Resolution Liquid Chromatography coupled with Electrospray Ionization Tandem Mass Spectrometry (RRLC-ESI-MSn) was used to separate and identify related substances in ampicillin capsule. The fragmentation behaviors of related substances were used to identify their chemical structures. Finally, a total of 13 related substances in ampicillin capsule were identified, including four identified components for the first time and three groups of isomers on the basis of the exact mass, fragmentation behaviors, retention time, and chemical structures in the literature. This study avoided time-consuming and complex chemosynthesis of related substances of ampicillin and the results could be useful for the quality control of ampicillin capsule to guarantee its safety in clinic. In the meantime, it provided a good example for the rapid identification of chemical structures of related substances of drugs. |
| format | Article |
| id | doaj-art-17d8150111e24d3784019b5e472ba074 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-17d8150111e24d3784019b5e472ba0742025-02-03T01:11:24ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732014-01-01201410.1155/2014/397492397492Identification of the Related Substances in Ampicillin Capsule by Rapid Resolution Liquid Chromatography Coupled with Electrospray Ionization Tandem Mass SpectrometryLei Zhang0Xian Long Cheng1Yang Liu2Miao Liang3Honghuan Dong4Beiran Lv5Wenning Yang6Zhiqiang Luo7Mingmin Tang8School of Chinese Materia Medica, Beijing University of Chinese Medicine, No. 6 Zhonghuan South Road, Wangjing, Chaoyang District, Beijing 100102, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, No. 6 Zhonghuan South Road, Wangjing, Chaoyang District, Beijing 100102, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, No. 6 Zhonghuan South Road, Wangjing, Chaoyang District, Beijing 100102, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, No. 6 Zhonghuan South Road, Wangjing, Chaoyang District, Beijing 100102, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, No. 6 Zhonghuan South Road, Wangjing, Chaoyang District, Beijing 100102, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, No. 6 Zhonghuan South Road, Wangjing, Chaoyang District, Beijing 100102, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, No. 6 Zhonghuan South Road, Wangjing, Chaoyang District, Beijing 100102, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, No. 6 Zhonghuan South Road, Wangjing, Chaoyang District, Beijing 100102, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, No. 6 Zhonghuan South Road, Wangjing, Chaoyang District, Beijing 100102, ChinaRapid Resolution Liquid Chromatography coupled with Electrospray Ionization Tandem Mass Spectrometry (RRLC-ESI-MSn) was used to separate and identify related substances in ampicillin capsule. The fragmentation behaviors of related substances were used to identify their chemical structures. Finally, a total of 13 related substances in ampicillin capsule were identified, including four identified components for the first time and three groups of isomers on the basis of the exact mass, fragmentation behaviors, retention time, and chemical structures in the literature. This study avoided time-consuming and complex chemosynthesis of related substances of ampicillin and the results could be useful for the quality control of ampicillin capsule to guarantee its safety in clinic. In the meantime, it provided a good example for the rapid identification of chemical structures of related substances of drugs.http://dx.doi.org/10.1155/2014/397492 |
| spellingShingle | Lei Zhang Xian Long Cheng Yang Liu Miao Liang Honghuan Dong Beiran Lv Wenning Yang Zhiqiang Luo Mingmin Tang Identification of the Related Substances in Ampicillin Capsule by Rapid Resolution Liquid Chromatography Coupled with Electrospray Ionization Tandem Mass Spectrometry Journal of Analytical Methods in Chemistry |
| title | Identification of the Related Substances in Ampicillin Capsule by Rapid Resolution Liquid Chromatography Coupled with Electrospray Ionization Tandem Mass Spectrometry |
| title_full | Identification of the Related Substances in Ampicillin Capsule by Rapid Resolution Liquid Chromatography Coupled with Electrospray Ionization Tandem Mass Spectrometry |
| title_fullStr | Identification of the Related Substances in Ampicillin Capsule by Rapid Resolution Liquid Chromatography Coupled with Electrospray Ionization Tandem Mass Spectrometry |
| title_full_unstemmed | Identification of the Related Substances in Ampicillin Capsule by Rapid Resolution Liquid Chromatography Coupled with Electrospray Ionization Tandem Mass Spectrometry |
| title_short | Identification of the Related Substances in Ampicillin Capsule by Rapid Resolution Liquid Chromatography Coupled with Electrospray Ionization Tandem Mass Spectrometry |
| title_sort | identification of the related substances in ampicillin capsule by rapid resolution liquid chromatography coupled with electrospray ionization tandem mass spectrometry |
| url | http://dx.doi.org/10.1155/2014/397492 |
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