Simultaneous Detemination of Atorvastatin Calcium and Amlodipine Besylate by Spectrophotometry and Multivariate Calibration Methods in Pharmaceutical Formulations
Resolution of binary mixture of atorvastatin (ATV) and amlodipine (AML) with minimum sample pretreatment and without analyte separation has been successfully achieved using a rapid method based on partial least square analysis of UV–spectral data. Multivariate calibration modeling procedures, tradit...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2011-01-01
|
| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2011/292346 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832564244126629888 |
|---|---|
| author | Amir H. M. Sarrafi Elahe Konoz Maryam Ghiyasvand |
| author_facet | Amir H. M. Sarrafi Elahe Konoz Maryam Ghiyasvand |
| author_sort | Amir H. M. Sarrafi |
| collection | DOAJ |
| description | Resolution of binary mixture of atorvastatin (ATV) and amlodipine (AML) with minimum sample pretreatment and without analyte separation has been successfully achieved using a rapid method based on partial least square analysis of UV–spectral data. Multivariate calibration modeling procedures, traditional partial least squares (PLS-2), interval partial least squares (iPLS) and synergy partial least squares (siPLS), were applied to select a spectral range that provided the lowest prediction error in comparison to the full-spectrum model. The simultaneous determination of both analytes was possible by PLS processing of sample absorbance between 220-425 nm. The correlation coefficients (R) and root mean squared error of cross validation (RMSECV) for ATV and AML in synthetic mixture were 0.9991, 0.9958 and 0.4538, 0.2411 in best siPLS models respectively. The optimized method has been used for determination of ATV and AML in amostatin commercial tablets. The proposed method are simple, fast, inexpensive and do not need any separation or preparation methods. |
| format | Article |
| id | doaj-art-17667da2bb1a48918f584ff768bbbc1d |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-17667da2bb1a48918f584ff768bbbc1d2025-02-03T01:11:28ZengWileyE-Journal of Chemistry0973-49452090-98102011-01-01841670167910.1155/2011/292346Simultaneous Detemination of Atorvastatin Calcium and Amlodipine Besylate by Spectrophotometry and Multivariate Calibration Methods in Pharmaceutical FormulationsAmir H. M. Sarrafi0Elahe Konoz1Maryam Ghiyasvand2Department of Chemistry, Faculty of Science, Islamic Azad University, Central Tehran Branch, P. O. Box 13185-768, Tehran, IranDepartment of Chemistry, Faculty of Science, Islamic Azad University, Central Tehran Branch, P. O. Box 13185-768, Tehran, IranDepartment of Chemistry, Faculty of Science, Islamic Azad University, Central Tehran Branch, P. O. Box 13185-768, Tehran, IranResolution of binary mixture of atorvastatin (ATV) and amlodipine (AML) with minimum sample pretreatment and without analyte separation has been successfully achieved using a rapid method based on partial least square analysis of UV–spectral data. Multivariate calibration modeling procedures, traditional partial least squares (PLS-2), interval partial least squares (iPLS) and synergy partial least squares (siPLS), were applied to select a spectral range that provided the lowest prediction error in comparison to the full-spectrum model. The simultaneous determination of both analytes was possible by PLS processing of sample absorbance between 220-425 nm. The correlation coefficients (R) and root mean squared error of cross validation (RMSECV) for ATV and AML in synthetic mixture were 0.9991, 0.9958 and 0.4538, 0.2411 in best siPLS models respectively. The optimized method has been used for determination of ATV and AML in amostatin commercial tablets. The proposed method are simple, fast, inexpensive and do not need any separation or preparation methods.http://dx.doi.org/10.1155/2011/292346 |
| spellingShingle | Amir H. M. Sarrafi Elahe Konoz Maryam Ghiyasvand Simultaneous Detemination of Atorvastatin Calcium and Amlodipine Besylate by Spectrophotometry and Multivariate Calibration Methods in Pharmaceutical Formulations E-Journal of Chemistry |
| title | Simultaneous Detemination of Atorvastatin Calcium and Amlodipine Besylate by Spectrophotometry and Multivariate Calibration Methods in Pharmaceutical Formulations |
| title_full | Simultaneous Detemination of Atorvastatin Calcium and Amlodipine Besylate by Spectrophotometry and Multivariate Calibration Methods in Pharmaceutical Formulations |
| title_fullStr | Simultaneous Detemination of Atorvastatin Calcium and Amlodipine Besylate by Spectrophotometry and Multivariate Calibration Methods in Pharmaceutical Formulations |
| title_full_unstemmed | Simultaneous Detemination of Atorvastatin Calcium and Amlodipine Besylate by Spectrophotometry and Multivariate Calibration Methods in Pharmaceutical Formulations |
| title_short | Simultaneous Detemination of Atorvastatin Calcium and Amlodipine Besylate by Spectrophotometry and Multivariate Calibration Methods in Pharmaceutical Formulations |
| title_sort | simultaneous detemination of atorvastatin calcium and amlodipine besylate by spectrophotometry and multivariate calibration methods in pharmaceutical formulations |
| url | http://dx.doi.org/10.1155/2011/292346 |
| work_keys_str_mv | AT amirhmsarrafi simultaneousdeteminationofatorvastatincalciumandamlodipinebesylatebyspectrophotometryandmultivariatecalibrationmethodsinpharmaceuticalformulations AT elahekonoz simultaneousdeteminationofatorvastatincalciumandamlodipinebesylatebyspectrophotometryandmultivariatecalibrationmethodsinpharmaceuticalformulations AT maryamghiyasvand simultaneousdeteminationofatorvastatincalciumandamlodipinebesylatebyspectrophotometryandmultivariatecalibrationmethodsinpharmaceuticalformulations |