Thymic Stromal Lymphopoietin Is Implicated in the Pathogenesis of Bullous Pemphigoid by Dendritic Cells
Objectives. Both thymic stromal lymphopoietin (TSLP) and dendritic cells (DCs) are involved in many autoimmune diseases, but the potential roles of TSLP and DCs in bullous pemphigoid (BP) have not been clarified. We sought to explore the contributions of TSLP and DCs in patients with BP. Methods. TS...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2020/4594630 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832560297194291200 |
|---|---|
| author | Si-Zhe Li Xin-Xing Jin Xiao-Lei Ge Ya-Gang Zuo Hong-Zhong Jin |
| author_facet | Si-Zhe Li Xin-Xing Jin Xiao-Lei Ge Ya-Gang Zuo Hong-Zhong Jin |
| author_sort | Si-Zhe Li |
| collection | DOAJ |
| description | Objectives. Both thymic stromal lymphopoietin (TSLP) and dendritic cells (DCs) are involved in many autoimmune diseases, but the potential roles of TSLP and DCs in bullous pemphigoid (BP) have not been clarified. We sought to explore the contributions of TSLP and DCs in patients with BP. Methods. TSLP levels in sera and blister fluids were measured by enzyme-linked immunosorbent assay. The TSLP expression in the BP lesional skin was detected by immunohistochemical staining. Infiltration of DCs, marked by DC-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN), and its relationship with TSLP and TSLP receptors was evaluated by immunofluorescence staining. Results. We found that TSLP levels in sera and in blister fluids of patients with BP were higher compared to the control groups. In patients with BP, TSLP levels in sera correlated with TSLP levels in blisters. The expression of TSLP in the BP lesional skin was higher compared to the healthy controls’ skin. Greater numbers of TSLP-positive cells were observed in the epidermis of patients with BP compared to the healthy controls. Greater numbers of DC-SIGN-positive cells were present in the BP lesional skin compared to the skin of controls. The expression of TSLP was highly upregulated in DC-SIGN-positive cells, and most DC-SIGN-positive cells expressed TSLP receptors. Conclusions. We conclude that TSLP may activate DC-SIGN-positive DCs directly, which may be involved in the pathogenesis of BP. |
| format | Article |
| id | doaj-art-175b9a8276174ee6b502e57bad4ec344 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-175b9a8276174ee6b502e57bad4ec3442025-02-03T01:27:54ZengWileyJournal of Immunology Research2314-88612314-71562020-01-01202010.1155/2020/45946304594630Thymic Stromal Lymphopoietin Is Implicated in the Pathogenesis of Bullous Pemphigoid by Dendritic CellsSi-Zhe Li0Xin-Xing Jin1Xiao-Lei Ge2Ya-Gang Zuo3Hong-Zhong Jin4Department of Dermatology, National Clinical Research Center for Dermatologic and Immunologic Diseases, Translational medicine Center, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaDepartment of Dermatology, National Clinical Research Center for Dermatologic and Immunologic Diseases, Translational medicine Center, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaDepartment of Dermatology, National Clinical Research Center for Dermatologic and Immunologic Diseases, Translational medicine Center, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaDepartment of Dermatology, National Clinical Research Center for Dermatologic and Immunologic Diseases, Translational medicine Center, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaDepartment of Dermatology, National Clinical Research Center for Dermatologic and Immunologic Diseases, Translational medicine Center, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaObjectives. Both thymic stromal lymphopoietin (TSLP) and dendritic cells (DCs) are involved in many autoimmune diseases, but the potential roles of TSLP and DCs in bullous pemphigoid (BP) have not been clarified. We sought to explore the contributions of TSLP and DCs in patients with BP. Methods. TSLP levels in sera and blister fluids were measured by enzyme-linked immunosorbent assay. The TSLP expression in the BP lesional skin was detected by immunohistochemical staining. Infiltration of DCs, marked by DC-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN), and its relationship with TSLP and TSLP receptors was evaluated by immunofluorescence staining. Results. We found that TSLP levels in sera and in blister fluids of patients with BP were higher compared to the control groups. In patients with BP, TSLP levels in sera correlated with TSLP levels in blisters. The expression of TSLP in the BP lesional skin was higher compared to the healthy controls’ skin. Greater numbers of TSLP-positive cells were observed in the epidermis of patients with BP compared to the healthy controls. Greater numbers of DC-SIGN-positive cells were present in the BP lesional skin compared to the skin of controls. The expression of TSLP was highly upregulated in DC-SIGN-positive cells, and most DC-SIGN-positive cells expressed TSLP receptors. Conclusions. We conclude that TSLP may activate DC-SIGN-positive DCs directly, which may be involved in the pathogenesis of BP.http://dx.doi.org/10.1155/2020/4594630 |
| spellingShingle | Si-Zhe Li Xin-Xing Jin Xiao-Lei Ge Ya-Gang Zuo Hong-Zhong Jin Thymic Stromal Lymphopoietin Is Implicated in the Pathogenesis of Bullous Pemphigoid by Dendritic Cells Journal of Immunology Research |
| title | Thymic Stromal Lymphopoietin Is Implicated in the Pathogenesis of Bullous Pemphigoid by Dendritic Cells |
| title_full | Thymic Stromal Lymphopoietin Is Implicated in the Pathogenesis of Bullous Pemphigoid by Dendritic Cells |
| title_fullStr | Thymic Stromal Lymphopoietin Is Implicated in the Pathogenesis of Bullous Pemphigoid by Dendritic Cells |
| title_full_unstemmed | Thymic Stromal Lymphopoietin Is Implicated in the Pathogenesis of Bullous Pemphigoid by Dendritic Cells |
| title_short | Thymic Stromal Lymphopoietin Is Implicated in the Pathogenesis of Bullous Pemphigoid by Dendritic Cells |
| title_sort | thymic stromal lymphopoietin is implicated in the pathogenesis of bullous pemphigoid by dendritic cells |
| url | http://dx.doi.org/10.1155/2020/4594630 |
| work_keys_str_mv | AT sizheli thymicstromallymphopoietinisimplicatedinthepathogenesisofbullouspemphigoidbydendriticcells AT xinxingjin thymicstromallymphopoietinisimplicatedinthepathogenesisofbullouspemphigoidbydendriticcells AT xiaoleige thymicstromallymphopoietinisimplicatedinthepathogenesisofbullouspemphigoidbydendriticcells AT yagangzuo thymicstromallymphopoietinisimplicatedinthepathogenesisofbullouspemphigoidbydendriticcells AT hongzhongjin thymicstromallymphopoietinisimplicatedinthepathogenesisofbullouspemphigoidbydendriticcells |