Gut microbiota interacting with vitamin D but not anandamide might contribute to the pathogenesis of preeclampsia: a preliminary study
IntroductionPreeclampsia (PE) is a pregnancy-specific multisystem disorder and a leading cause of maternal and perinatal mortality globally. Despite numerous studies highlighting the potential roles of gut microbiota, anandamide (AEA), and Vitamin D (VitD) in PE, none have established them as reliab...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1469054/full |
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| author | Xiao-Qiang Han Xiao-Qiang Han Hui-Hui Jiang Meng-Ling Chen Meng-Ling Chen De-Yang Han De-Yang Han Su-Fen Zhou Su-Fen Zhou Jin-Wen Wang Shu-Shen Ji Ling-Yun Wang Jing-Wei Lou Ming-Qun Li Ming-Qun Li |
| author_facet | Xiao-Qiang Han Xiao-Qiang Han Hui-Hui Jiang Meng-Ling Chen Meng-Ling Chen De-Yang Han De-Yang Han Su-Fen Zhou Su-Fen Zhou Jin-Wen Wang Shu-Shen Ji Ling-Yun Wang Jing-Wei Lou Ming-Qun Li Ming-Qun Li |
| author_sort | Xiao-Qiang Han |
| collection | DOAJ |
| description | IntroductionPreeclampsia (PE) is a pregnancy-specific multisystem disorder and a leading cause of maternal and perinatal mortality globally. Despite numerous studies highlighting the potential roles of gut microbiota, anandamide (AEA), and Vitamin D (VitD) in PE, none have established them as reliable biomarkers for predicting disease onset. Moreover, their interactions in late-stage pregnancy women remain poorly understood.MethodsThirty-four preeclamptic patients (called PE group) and thirty-nine matched healthy late-pregnant women (called LP group) were involved in this case-control study. Fecal samples, which were used to acquire the diversity and composition of gut microbiota, were analyzed by 16S rRNA gene sequencing. Plasma AEA concentrations and serum VitD levels were determined by high-performance liquid chromatography-mass spectrometry (HPLC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS), respectively.ResultsIn this study, β diversity but not α diversity significantly differed between the LP and PE groups. Compared with the LP group, the relative abundances of Prevotella, Erysipelotrichaceae_UCG-003, and Dorea were increased dramatically in the PE group, whereas the relative abundances of Subdoligranulum, Parabacteroides, Bacteroides were significantly decreased in the PE group. Furthermore, women with PE had a substantially lower plasma level of AEA and a marked decrease in serum VitD compared to normal late-pregnant women. Lastly, although the serum level of AEA was not significantly correlated with VitD or any of the top 6 marker genera, VitD was significantly negatively correlated with the relative abundance of Dorea, a novel finding in this context.DiscussionThe gut microbiota profile of the PE group was significantly different from that of the LP group. Although no significant correlations were identified between the plasma AEA levels and serum VitD levels or any of the top 6 identified marker genera, a significant negative correlation was observed between VitD and Dorea, indicating VitD and gut microbiota have the potential to be combined targets for early diagnosis and management of PE. |
| format | Article |
| id | doaj-art-1758ec20d2f741069044ef4edf94e3f7 |
| institution | Kabale University |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-1758ec20d2f741069044ef4edf94e3f72025-02-05T07:32:37ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-02-011410.3389/fcimb.2024.14690541469054Gut microbiota interacting with vitamin D but not anandamide might contribute to the pathogenesis of preeclampsia: a preliminary studyXiao-Qiang Han0Xiao-Qiang Han1Hui-Hui Jiang2Meng-Ling Chen3Meng-Ling Chen4De-Yang Han5De-Yang Han6Su-Fen Zhou7Su-Fen Zhou8Jin-Wen Wang9Shu-Shen Ji10Ling-Yun Wang11Jing-Wei Lou12Ming-Qun Li13Ming-Qun Li14Department of Obstetrics and Gynaecology, Xiangyang No. 1 People’s Hospital, Hubei University of Medicine, Xiangyang, ChinaHubei Provincial Clinical Research Center for Accurate Fetus Malformation Diagnosis, Xiangyang, ChinaZhangjiang Center for Translational Medicine, Shanghai Biotecan Pharmaceuticals Co., Ltd., Shanghai, ChinaDepartment of Obstetrics and Gynaecology, Xiangyang No. 1 People’s Hospital, Hubei University of Medicine, Xiangyang, ChinaHubei Provincial Clinical Research Center for Accurate Fetus Malformation Diagnosis, Xiangyang, ChinaDepartment of Obstetrics and Gynaecology, Xiangyang No. 1 People’s Hospital, Hubei University of Medicine, Xiangyang, ChinaHubei Provincial Clinical Research Center for Accurate Fetus Malformation Diagnosis, Xiangyang, ChinaHubei Provincial Clinical Research Center for Accurate Fetus Malformation Diagnosis, Xiangyang, ChinaDepartment of Ultrasound, Xiangyang No. 1 People’s Hospital, Hubei University of Medicine, Xiangyang, ChinaZhangjiang Center for Translational Medicine, Shanghai Biotecan Pharmaceuticals Co., Ltd., Shanghai, ChinaZhangjiang Center for Translational Medicine, Shanghai Biotecan Pharmaceuticals Co., Ltd., Shanghai, ChinaZhangjiang Center for Translational Medicine, Shanghai Biotecan Pharmaceuticals Co., Ltd., Shanghai, ChinaZhangjiang Center for Translational Medicine, Shanghai Biotecan Pharmaceuticals Co., Ltd., Shanghai, ChinaDepartment of Obstetrics and Gynaecology, Xiangyang No. 1 People’s Hospital, Hubei University of Medicine, Xiangyang, ChinaHubei Provincial Clinical Research Center for Accurate Fetus Malformation Diagnosis, Xiangyang, ChinaIntroductionPreeclampsia (PE) is a pregnancy-specific multisystem disorder and a leading cause of maternal and perinatal mortality globally. Despite numerous studies highlighting the potential roles of gut microbiota, anandamide (AEA), and Vitamin D (VitD) in PE, none have established them as reliable biomarkers for predicting disease onset. Moreover, their interactions in late-stage pregnancy women remain poorly understood.MethodsThirty-four preeclamptic patients (called PE group) and thirty-nine matched healthy late-pregnant women (called LP group) were involved in this case-control study. Fecal samples, which were used to acquire the diversity and composition of gut microbiota, were analyzed by 16S rRNA gene sequencing. Plasma AEA concentrations and serum VitD levels were determined by high-performance liquid chromatography-mass spectrometry (HPLC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS), respectively.ResultsIn this study, β diversity but not α diversity significantly differed between the LP and PE groups. Compared with the LP group, the relative abundances of Prevotella, Erysipelotrichaceae_UCG-003, and Dorea were increased dramatically in the PE group, whereas the relative abundances of Subdoligranulum, Parabacteroides, Bacteroides were significantly decreased in the PE group. Furthermore, women with PE had a substantially lower plasma level of AEA and a marked decrease in serum VitD compared to normal late-pregnant women. Lastly, although the serum level of AEA was not significantly correlated with VitD or any of the top 6 marker genera, VitD was significantly negatively correlated with the relative abundance of Dorea, a novel finding in this context.DiscussionThe gut microbiota profile of the PE group was significantly different from that of the LP group. Although no significant correlations were identified between the plasma AEA levels and serum VitD levels or any of the top 6 identified marker genera, a significant negative correlation was observed between VitD and Dorea, indicating VitD and gut microbiota have the potential to be combined targets for early diagnosis and management of PE.https://www.frontiersin.org/articles/10.3389/fcimb.2024.1469054/fullgut microbiotaanandamidevitamin Dpreeclampsiainflammationinteraction |
| spellingShingle | Xiao-Qiang Han Xiao-Qiang Han Hui-Hui Jiang Meng-Ling Chen Meng-Ling Chen De-Yang Han De-Yang Han Su-Fen Zhou Su-Fen Zhou Jin-Wen Wang Shu-Shen Ji Ling-Yun Wang Jing-Wei Lou Ming-Qun Li Ming-Qun Li Gut microbiota interacting with vitamin D but not anandamide might contribute to the pathogenesis of preeclampsia: a preliminary study Frontiers in Cellular and Infection Microbiology gut microbiota anandamide vitamin D preeclampsia inflammation interaction |
| title | Gut microbiota interacting with vitamin D but not anandamide might contribute to the pathogenesis of preeclampsia: a preliminary study |
| title_full | Gut microbiota interacting with vitamin D but not anandamide might contribute to the pathogenesis of preeclampsia: a preliminary study |
| title_fullStr | Gut microbiota interacting with vitamin D but not anandamide might contribute to the pathogenesis of preeclampsia: a preliminary study |
| title_full_unstemmed | Gut microbiota interacting with vitamin D but not anandamide might contribute to the pathogenesis of preeclampsia: a preliminary study |
| title_short | Gut microbiota interacting with vitamin D but not anandamide might contribute to the pathogenesis of preeclampsia: a preliminary study |
| title_sort | gut microbiota interacting with vitamin d but not anandamide might contribute to the pathogenesis of preeclampsia a preliminary study |
| topic | gut microbiota anandamide vitamin D preeclampsia inflammation interaction |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1469054/full |
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