Antibacterial activity of 1 % Roselle flower nano-emulsion extract (Hibiscus sabdariffa) against peri-implantitis-related bacteria on orthodontic mini-implants: An in vitro study
Objective: The orthodontic mini-implant (OMI) failure often occurs due to the accumulation of peri-implantitis bacteria surrounding it, which results in a stable, resistant form of absolute skeletal anchorage during orthodontic treatment. Administering doxycycline may be the solution, but long-term...
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| Language: | English |
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Elsevier
2025-01-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426824001933 |
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| author | Ridhofar Akbar Khusnul Abdillah Theresia Indah Budhy Ida Bagus Narmada I Gusti Aju Wahju Ardani Nastiti Faradilla Ramadhani Ratri Maya Sitalaksmi Muhammad Luthfi Ninik Mas Ulfa Albertus Putera Nugraha Tengku Natasha Eleena binti Tengku Ahmad Noor Alexander Patera Nugraha |
| author_facet | Ridhofar Akbar Khusnul Abdillah Theresia Indah Budhy Ida Bagus Narmada I Gusti Aju Wahju Ardani Nastiti Faradilla Ramadhani Ratri Maya Sitalaksmi Muhammad Luthfi Ninik Mas Ulfa Albertus Putera Nugraha Tengku Natasha Eleena binti Tengku Ahmad Noor Alexander Patera Nugraha |
| author_sort | Ridhofar Akbar Khusnul Abdillah |
| collection | DOAJ |
| description | Objective: The orthodontic mini-implant (OMI) failure often occurs due to the accumulation of peri-implantitis bacteria surrounding it, which results in a stable, resistant form of absolute skeletal anchorage during orthodontic treatment. Administering doxycycline may be the solution, but long-term side effects result in antibiotic resistance. Roselle flowers (Hibiscus sabdariffa) possess beneficial active phytochemical substances, which may have potential as an OMI peri-implantitis alternative therapy. This study investigates the antibacterial activity of 1 % Roselle flower (H. sabdarifa) nanoemulsion (NE) extract (1 % RNE) toward peri-implantitis bacteria in OMIs. Methods: A phytochemical analysis of 1 % RNE was carried out to examine the active substances possessed in it, such as flavonoids, quinone, saponin, alkaloids, tannins, terpenoids, and steroids. Nanoemulsion characterization was carried out using a particle size analyzer (PSA). The antibacterial activity of 1 % RNE toward Prevotella intermedia (Pi), Porphyromonas gingivalis (Pg), Aggregatibacter actinomycetemcomitans (Aa), and Fusobacterium nucleatum (Fn) was carried out to determine the minimum inhibitory concentration, minimum bactericidal concentration, and inhibitory zone compared with doxycycline as a positive control. Results: Roselle flower NE extract (1 %) possessed flavonoids, quinone, saponin, alkaloids, tannins, terpenoids, and steroids positively. The PSA showed that the 1 % RNE had a size of 98.13 d nm. The antibacterial activity of 1 % RNE against Aa, Pg, Pi, and Fn bacteria at 3.125 % showed significant differences (p < 0.05). The antibacterial activity of 1 % RNE toward peri-implantitis bacteria is lower than that of doxycycline. Conclusion: Roselle flower NE extract (1 %) has antibacterial activity against peri-implantitis bacteria at a concentration of 3.125 %. |
| format | Article |
| id | doaj-art-1732ed8cb12945adb841d639ebfe4b73 |
| institution | Kabale University |
| issn | 2212-4268 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-1732ed8cb12945adb841d639ebfe4b732025-02-09T05:00:14ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682025-01-01151170177Antibacterial activity of 1 % Roselle flower nano-emulsion extract (Hibiscus sabdariffa) against peri-implantitis-related bacteria on orthodontic mini-implants: An in vitro studyRidhofar Akbar Khusnul Abdillah0Theresia Indah Budhy1Ida Bagus Narmada2I Gusti Aju Wahju Ardani3Nastiti Faradilla Ramadhani4Ratri Maya Sitalaksmi5Muhammad Luthfi6Ninik Mas Ulfa7Albertus Putera Nugraha8Tengku Natasha Eleena binti Tengku Ahmad Noor9Alexander Patera Nugraha10Graduate Student of Dental Health Science, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, IndonesiaOral and Maxillofacial Pathology Department, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, IndonesiaOrthodontic Department, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, IndonesiaOrthodontic Department, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, IndonesiaDentomaxillofacial Radiology Department, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, IndonesiaProsthodontic Department, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, IndonesiaOral Biology Department, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, IndonesiaPharmacy Academy, Surabaya, East Java, IndonesiaFaculty of Medicine, Universitas Airlangga, Surabaya, East Java, IndonesiaMilitary Dental Officer of Royal Medical and Dental Corps, Malaysian Armed Forces, Semenggo Camp, Kuching, Serawak, MalaysiaOrthodontic Department, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia; Corresponding author.Objective: The orthodontic mini-implant (OMI) failure often occurs due to the accumulation of peri-implantitis bacteria surrounding it, which results in a stable, resistant form of absolute skeletal anchorage during orthodontic treatment. Administering doxycycline may be the solution, but long-term side effects result in antibiotic resistance. Roselle flowers (Hibiscus sabdariffa) possess beneficial active phytochemical substances, which may have potential as an OMI peri-implantitis alternative therapy. This study investigates the antibacterial activity of 1 % Roselle flower (H. sabdarifa) nanoemulsion (NE) extract (1 % RNE) toward peri-implantitis bacteria in OMIs. Methods: A phytochemical analysis of 1 % RNE was carried out to examine the active substances possessed in it, such as flavonoids, quinone, saponin, alkaloids, tannins, terpenoids, and steroids. Nanoemulsion characterization was carried out using a particle size analyzer (PSA). The antibacterial activity of 1 % RNE toward Prevotella intermedia (Pi), Porphyromonas gingivalis (Pg), Aggregatibacter actinomycetemcomitans (Aa), and Fusobacterium nucleatum (Fn) was carried out to determine the minimum inhibitory concentration, minimum bactericidal concentration, and inhibitory zone compared with doxycycline as a positive control. Results: Roselle flower NE extract (1 %) possessed flavonoids, quinone, saponin, alkaloids, tannins, terpenoids, and steroids positively. The PSA showed that the 1 % RNE had a size of 98.13 d nm. The antibacterial activity of 1 % RNE against Aa, Pg, Pi, and Fn bacteria at 3.125 % showed significant differences (p < 0.05). The antibacterial activity of 1 % RNE toward peri-implantitis bacteria is lower than that of doxycycline. Conclusion: Roselle flower NE extract (1 %) has antibacterial activity against peri-implantitis bacteria at a concentration of 3.125 %.http://www.sciencedirect.com/science/article/pii/S2212426824001933OrthodonticHibiscus sabdarifaNanoemulsionPeri-implantitisMedicine |
| spellingShingle | Ridhofar Akbar Khusnul Abdillah Theresia Indah Budhy Ida Bagus Narmada I Gusti Aju Wahju Ardani Nastiti Faradilla Ramadhani Ratri Maya Sitalaksmi Muhammad Luthfi Ninik Mas Ulfa Albertus Putera Nugraha Tengku Natasha Eleena binti Tengku Ahmad Noor Alexander Patera Nugraha Antibacterial activity of 1 % Roselle flower nano-emulsion extract (Hibiscus sabdariffa) against peri-implantitis-related bacteria on orthodontic mini-implants: An in vitro study Journal of Oral Biology and Craniofacial Research Orthodontic Hibiscus sabdarifa Nanoemulsion Peri-implantitis Medicine |
| title | Antibacterial activity of 1 % Roselle flower nano-emulsion extract (Hibiscus sabdariffa) against peri-implantitis-related bacteria on orthodontic mini-implants: An in vitro study |
| title_full | Antibacterial activity of 1 % Roselle flower nano-emulsion extract (Hibiscus sabdariffa) against peri-implantitis-related bacteria on orthodontic mini-implants: An in vitro study |
| title_fullStr | Antibacterial activity of 1 % Roselle flower nano-emulsion extract (Hibiscus sabdariffa) against peri-implantitis-related bacteria on orthodontic mini-implants: An in vitro study |
| title_full_unstemmed | Antibacterial activity of 1 % Roselle flower nano-emulsion extract (Hibiscus sabdariffa) against peri-implantitis-related bacteria on orthodontic mini-implants: An in vitro study |
| title_short | Antibacterial activity of 1 % Roselle flower nano-emulsion extract (Hibiscus sabdariffa) against peri-implantitis-related bacteria on orthodontic mini-implants: An in vitro study |
| title_sort | antibacterial activity of 1 roselle flower nano emulsion extract hibiscus sabdariffa against peri implantitis related bacteria on orthodontic mini implants an in vitro study |
| topic | Orthodontic Hibiscus sabdarifa Nanoemulsion Peri-implantitis Medicine |
| url | http://www.sciencedirect.com/science/article/pii/S2212426824001933 |
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