The grapevine ABC transporter B family member 15 is a trans-resveratrol transporter out of grapevine cells
Stilbenes, particularly trans-resveratrol, play a highly relevant defense role in grapevines as phytoalexin is induced in response to stress. Metabolism and transport of stilbenes can be conveniently investigated in grapevine cell culture since large amounts of trans-resveratrol are accumulated in t...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1450638/full |
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| author | Ascensión Martínez-Márquez Ascensión Martínez-Márquez Viviana Martins Susana Sellés-Marchart Susana Sellés-Marchart Hernâni Gerós Purificación Corchete Roque Bru-Martínez Roque Bru-Martínez Roque Bru-Martínez |
| author_facet | Ascensión Martínez-Márquez Ascensión Martínez-Márquez Viviana Martins Susana Sellés-Marchart Susana Sellés-Marchart Hernâni Gerós Purificación Corchete Roque Bru-Martínez Roque Bru-Martínez Roque Bru-Martínez |
| author_sort | Ascensión Martínez-Márquez |
| collection | DOAJ |
| description | Stilbenes, particularly trans-resveratrol, play a highly relevant defense role in grapevines as phytoalexin is induced in response to stress. Metabolism and transport of stilbenes can be conveniently investigated in grapevine cell culture since large amounts of trans-resveratrol are accumulated in the extracellular medium upon treatment with the elicitor methylated cyclodextrin, either alone or combined with methyl jasmonate. A proteomic approach on grapevine cell membrane fractions was performed to find trans-resveratrol transporter candidates. The candidate VvABCB15 was functionally characterized. Its stable expression in both yeast and Silybum marianum cells’ heterologous systems led to increased trans-resveratrol transport in these hosts. Transient expression in Vitis cells showed an enhanced absorbent- or elicitor-assisted accumulation of extracellular trans-resveratrol in VvABCB15-expressing or VvGSTU10/VvABCB15-co-expressing cell suspension cultures. Experiments of transient expression in Vitis cell suspensions using light-switchable stilbene synthase (pHYH::VvSTS3) and VvABCB15 further confirmed the candidate’s role as a trans-resveratrol transporter. VvABCB15-YFP fusion proteins in Nicotiana leaf showed localization in the plasma membrane, consistent with a functional role in trans-resveratrol transport. This is the first report to provide evidence for the involvement of an ABC transporter B type, VvABCB15, in trans-resveratrol transport to the extracellular medium of grapevine cells. |
| format | Article |
| id | doaj-art-172db67814c342f48ff2d3bddedad50d |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-172db67814c342f48ff2d3bddedad50d2025-01-21T15:25:27ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-01-011510.3389/fpls.2024.14506381450638The grapevine ABC transporter B family member 15 is a trans-resveratrol transporter out of grapevine cellsAscensión Martínez-Márquez0Ascensión Martínez-Márquez1Viviana Martins2Susana Sellés-Marchart3Susana Sellés-Marchart4Hernâni Gerós5Purificación Corchete6Roque Bru-Martínez7Roque Bru-Martínez8Roque Bru-Martínez9Plant Proteomics and Functional Genomics Group, Department of Biochemistry and Molecular Biology and Soil Science and Agricultural Chemistry, Faculty of Science, University of Alicante, Alicante, SpainAlicante Institute for Health and Biomedical Research (ISABIAL), Alicante, SpainCentre of Molecular and Environmental Biology, Department of Biology, University of Minho, de Gualtar, Braga, PortugalPlant Proteomics and Functional Genomics Group, Department of Biochemistry and Molecular Biology and Soil Science and Agricultural Chemistry, Faculty of Science, University of Alicante, Alicante, SpainResearch Technical Facility, Proteomics and Genomics Division, University of Alicante, Alicante, SpainCentre of Molecular and Environmental Biology, Department of Biology, University of Minho, de Gualtar, Braga, PortugalDepartment of Plant Physiology, Miguel de Unamuno, University of Salamanca, Salamanca, SpainPlant Proteomics and Functional Genomics Group, Department of Biochemistry and Molecular Biology and Soil Science and Agricultural Chemistry, Faculty of Science, University of Alicante, Alicante, SpainAlicante Institute for Health and Biomedical Research (ISABIAL), Alicante, SpainMultidisciplinary Institute for the Study of the Environment (IMEM), University of Alicante, Alicante, SpainStilbenes, particularly trans-resveratrol, play a highly relevant defense role in grapevines as phytoalexin is induced in response to stress. Metabolism and transport of stilbenes can be conveniently investigated in grapevine cell culture since large amounts of trans-resveratrol are accumulated in the extracellular medium upon treatment with the elicitor methylated cyclodextrin, either alone or combined with methyl jasmonate. A proteomic approach on grapevine cell membrane fractions was performed to find trans-resveratrol transporter candidates. The candidate VvABCB15 was functionally characterized. Its stable expression in both yeast and Silybum marianum cells’ heterologous systems led to increased trans-resveratrol transport in these hosts. Transient expression in Vitis cells showed an enhanced absorbent- or elicitor-assisted accumulation of extracellular trans-resveratrol in VvABCB15-expressing or VvGSTU10/VvABCB15-co-expressing cell suspension cultures. Experiments of transient expression in Vitis cell suspensions using light-switchable stilbene synthase (pHYH::VvSTS3) and VvABCB15 further confirmed the candidate’s role as a trans-resveratrol transporter. VvABCB15-YFP fusion proteins in Nicotiana leaf showed localization in the plasma membrane, consistent with a functional role in trans-resveratrol transport. This is the first report to provide evidence for the involvement of an ABC transporter B type, VvABCB15, in trans-resveratrol transport to the extracellular medium of grapevine cells.https://www.frontiersin.org/articles/10.3389/fpls.2024.1450638/fullATP binding cassette (ABC) transporter B family member 15label-free proteomics analysisgrapevine cell cultureresveratroltransformationtransport |
| spellingShingle | Ascensión Martínez-Márquez Ascensión Martínez-Márquez Viviana Martins Susana Sellés-Marchart Susana Sellés-Marchart Hernâni Gerós Purificación Corchete Roque Bru-Martínez Roque Bru-Martínez Roque Bru-Martínez The grapevine ABC transporter B family member 15 is a trans-resveratrol transporter out of grapevine cells Frontiers in Plant Science ATP binding cassette (ABC) transporter B family member 15 label-free proteomics analysis grapevine cell culture resveratrol transformation transport |
| title | The grapevine ABC transporter B family member 15 is a trans-resveratrol transporter out of grapevine cells |
| title_full | The grapevine ABC transporter B family member 15 is a trans-resveratrol transporter out of grapevine cells |
| title_fullStr | The grapevine ABC transporter B family member 15 is a trans-resveratrol transporter out of grapevine cells |
| title_full_unstemmed | The grapevine ABC transporter B family member 15 is a trans-resveratrol transporter out of grapevine cells |
| title_short | The grapevine ABC transporter B family member 15 is a trans-resveratrol transporter out of grapevine cells |
| title_sort | grapevine abc transporter b family member 15 is a trans resveratrol transporter out of grapevine cells |
| topic | ATP binding cassette (ABC) transporter B family member 15 label-free proteomics analysis grapevine cell culture resveratrol transformation transport |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1450638/full |
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