Optimization of Process Parameters for ε-Polylysine Production by Response Surface Methods
ε-Polylysine (ε-PL) is a highly safe natural food preservative with a broad antimicrobial spectrum, excellent corrosion resistances, and great commercial potentials. In the present work, we evaluated the ε-PL adsorption performances of HZB-3B and D155 resins and optimized the adsorption and desorpti...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2016/3785036 |
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| _version_ | 1832563687868596224 |
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| author | Maxiaoqi Zhu Zhicai Zhang Yiqiuyi Liu Feng Wang Lili Xia Jianwei Xia Hongming Guo |
| author_facet | Maxiaoqi Zhu Zhicai Zhang Yiqiuyi Liu Feng Wang Lili Xia Jianwei Xia Hongming Guo |
| author_sort | Maxiaoqi Zhu |
| collection | DOAJ |
| description | ε-Polylysine (ε-PL) is a highly safe natural food preservative with a broad antimicrobial spectrum, excellent corrosion resistances, and great commercial potentials. In the present work, we evaluated the ε-PL adsorption performances of HZB-3B and D155 resins and optimized the adsorption and desorption conditions by single-factor test, response surface method, and orthogonal design. The complexes of resin and ε-PL were characterized by SEM and FITR. The results indicated that D155 resin had the best ε-PL adsorption performance and was selected for the separation and purification of ε-PL. The conditions for the static adsorption of ε-PL on D155 resin were optimized as follows: ε-PL solution 40 g/L, pH 8.5, resins 15 g/L, and absorption time 14 h. The adsorption efficiency of ε-PL under the optimal conditions was 96.84%. The ε-PL adsorbed on the D155 resin was easily desorbed with 0.4 mol/L HCl at 30°C in 10 h. The highest desorption efficiency was 97.57% and the overall recovery of ε-PL was 94.49% under the optimal conditions. The excellent ε-PL adsorption and desorption properties of D155 resin including high selectivity and adsorption capacity, easy desorption, and high stability make it a good candidate for the isolation of ε-PL from fermentation broths. |
| format | Article |
| id | doaj-art-172da88b6e0445618df6ee12bde550f9 |
| institution | Kabale University |
| issn | 1687-9422 1687-9430 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Polymer Science |
| spelling | doaj-art-172da88b6e0445618df6ee12bde550f92025-02-03T01:12:49ZengWileyInternational Journal of Polymer Science1687-94221687-94302016-01-01201610.1155/2016/37850363785036Optimization of Process Parameters for ε-Polylysine Production by Response Surface MethodsMaxiaoqi Zhu0Zhicai Zhang1Yiqiuyi Liu2Feng Wang3Lili Xia4Jianwei Xia5Hongming Guo6School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, ChinaJiangsu Yiming Biological Co., Ltd., Taizhou, Jiangsu 223001, ChinaJiangsu Yiming Biological Co., Ltd., Taizhou, Jiangsu 223001, Chinaε-Polylysine (ε-PL) is a highly safe natural food preservative with a broad antimicrobial spectrum, excellent corrosion resistances, and great commercial potentials. In the present work, we evaluated the ε-PL adsorption performances of HZB-3B and D155 resins and optimized the adsorption and desorption conditions by single-factor test, response surface method, and orthogonal design. The complexes of resin and ε-PL were characterized by SEM and FITR. The results indicated that D155 resin had the best ε-PL adsorption performance and was selected for the separation and purification of ε-PL. The conditions for the static adsorption of ε-PL on D155 resin were optimized as follows: ε-PL solution 40 g/L, pH 8.5, resins 15 g/L, and absorption time 14 h. The adsorption efficiency of ε-PL under the optimal conditions was 96.84%. The ε-PL adsorbed on the D155 resin was easily desorbed with 0.4 mol/L HCl at 30°C in 10 h. The highest desorption efficiency was 97.57% and the overall recovery of ε-PL was 94.49% under the optimal conditions. The excellent ε-PL adsorption and desorption properties of D155 resin including high selectivity and adsorption capacity, easy desorption, and high stability make it a good candidate for the isolation of ε-PL from fermentation broths.http://dx.doi.org/10.1155/2016/3785036 |
| spellingShingle | Maxiaoqi Zhu Zhicai Zhang Yiqiuyi Liu Feng Wang Lili Xia Jianwei Xia Hongming Guo Optimization of Process Parameters for ε-Polylysine Production by Response Surface Methods International Journal of Polymer Science |
| title | Optimization of Process Parameters for ε-Polylysine Production by Response Surface Methods |
| title_full | Optimization of Process Parameters for ε-Polylysine Production by Response Surface Methods |
| title_fullStr | Optimization of Process Parameters for ε-Polylysine Production by Response Surface Methods |
| title_full_unstemmed | Optimization of Process Parameters for ε-Polylysine Production by Response Surface Methods |
| title_short | Optimization of Process Parameters for ε-Polylysine Production by Response Surface Methods |
| title_sort | optimization of process parameters for ε polylysine production by response surface methods |
| url | http://dx.doi.org/10.1155/2016/3785036 |
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