Manufacturing of Human Umbilical Cord Mesenchymal Stromal Cells on Microcarriers in a Dynamic System for Clinical Use
The great properties of human mesenchymal stromal cells (hMSCs) make these cells an important tool in regenerative medicine. Because of the limitations of hMSCs derived from the bone marrow during isolation and expansion, hMSCs derived from the umbilical cord stroma are a great alternative to overco...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/4834616 |
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| author | Florian Petry J. Robert Smith Jasmin Leber Denise Salzig Peter Czermak Mark L. Weiss |
| author_facet | Florian Petry J. Robert Smith Jasmin Leber Denise Salzig Peter Czermak Mark L. Weiss |
| author_sort | Florian Petry |
| collection | DOAJ |
| description | The great properties of human mesenchymal stromal cells (hMSCs) make these cells an important tool in regenerative medicine. Because of the limitations of hMSCs derived from the bone marrow during isolation and expansion, hMSCs derived from the umbilical cord stroma are a great alternative to overcome these issues. For a large expansion of these cells, we performed a process transfer from static culture to a dynamic system. For this reason, a microcarrier selection out of five microcarrier types was made to achieve a suitable growth surface for the cells. The growth characteristics and metabolite consumption and production were used to compare the cells growth in 12-well plate and spinner flask. The goal to determine relevant process parameters to transfer the expansion process into a stirred tank bioreactor was achieved. |
| format | Article |
| id | doaj-art-172c7a8ea5e34c9ca156fefed6efd0ca |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-172c7a8ea5e34c9ca156fefed6efd0ca2025-02-03T01:22:50ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/48346164834616Manufacturing of Human Umbilical Cord Mesenchymal Stromal Cells on Microcarriers in a Dynamic System for Clinical UseFlorian Petry0J. Robert Smith1Jasmin Leber2Denise Salzig3Peter Czermak4Mark L. Weiss5Department of Anatomy and Physiology, Kansas State University, 228 Coles Hall, Manhattan, KS 66506, USADepartment of Anatomy and Physiology, Kansas State University, 228 Coles Hall, Manhattan, KS 66506, USAInstitute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Wiesenstraße 14, 35390 Gießen, GermanyInstitute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Wiesenstraße 14, 35390 Gießen, GermanyInstitute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Wiesenstraße 14, 35390 Gießen, GermanyDepartment of Anatomy and Physiology, Kansas State University, 228 Coles Hall, Manhattan, KS 66506, USAThe great properties of human mesenchymal stromal cells (hMSCs) make these cells an important tool in regenerative medicine. Because of the limitations of hMSCs derived from the bone marrow during isolation and expansion, hMSCs derived from the umbilical cord stroma are a great alternative to overcome these issues. For a large expansion of these cells, we performed a process transfer from static culture to a dynamic system. For this reason, a microcarrier selection out of five microcarrier types was made to achieve a suitable growth surface for the cells. The growth characteristics and metabolite consumption and production were used to compare the cells growth in 12-well plate and spinner flask. The goal to determine relevant process parameters to transfer the expansion process into a stirred tank bioreactor was achieved.http://dx.doi.org/10.1155/2016/4834616 |
| spellingShingle | Florian Petry J. Robert Smith Jasmin Leber Denise Salzig Peter Czermak Mark L. Weiss Manufacturing of Human Umbilical Cord Mesenchymal Stromal Cells on Microcarriers in a Dynamic System for Clinical Use Stem Cells International |
| title | Manufacturing of Human Umbilical Cord Mesenchymal Stromal Cells on Microcarriers in a Dynamic System for Clinical Use |
| title_full | Manufacturing of Human Umbilical Cord Mesenchymal Stromal Cells on Microcarriers in a Dynamic System for Clinical Use |
| title_fullStr | Manufacturing of Human Umbilical Cord Mesenchymal Stromal Cells on Microcarriers in a Dynamic System for Clinical Use |
| title_full_unstemmed | Manufacturing of Human Umbilical Cord Mesenchymal Stromal Cells on Microcarriers in a Dynamic System for Clinical Use |
| title_short | Manufacturing of Human Umbilical Cord Mesenchymal Stromal Cells on Microcarriers in a Dynamic System for Clinical Use |
| title_sort | manufacturing of human umbilical cord mesenchymal stromal cells on microcarriers in a dynamic system for clinical use |
| url | http://dx.doi.org/10.1155/2016/4834616 |
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