Genome-Wide Expression Difference of MicroRNAs in Basal Cell Carcinoma
Distinct expression of the miRNAs has rarely been explored in basal cell carcinoma (BCC) of skin, and the regulatory role of miRNAs in BCC development remains quite opaque. Here, we collected control tissues from adjacent noncancerous skin (n=15; control group) and tissues at tumor centers from pati...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-01-01
|
| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2021/7223500 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832556868504911872 |
|---|---|
| author | Hai-Peng Wei Song Zhan Qing-An Zhu Zhen-Juan Chen Xian Feng Jun-Yuan Chen Qi-Lin Zhang Jingjie Zhao Lingzhang Meng |
| author_facet | Hai-Peng Wei Song Zhan Qing-An Zhu Zhen-Juan Chen Xian Feng Jun-Yuan Chen Qi-Lin Zhang Jingjie Zhao Lingzhang Meng |
| author_sort | Hai-Peng Wei |
| collection | DOAJ |
| description | Distinct expression of the miRNAs has rarely been explored in basal cell carcinoma (BCC) of skin, and the regulatory role of miRNAs in BCC development remains quite opaque. Here, we collected control tissues from adjacent noncancerous skin (n=15; control group) and tissues at tumor centers from patients with cheek BCC (n=15; BCC group) using punch biopsies. After six small RNA sequencing- (sRNA-seq-) based miRNA expression profiles were generated for both BCC and controls, including three biological replicates, we conducted comparative analysis on the sRNA-seq dataset, discovering 181 differentially expressed miRNAs (DEMs) out of the 1,873 miRNAs in BCCs. In order to validate the sRNA-seq data, expression of 15 randomly selected DEMs was measured using the TaqMan probe-based quantitative real-time PCR. Functional analysis of predicted target genes of DEMs in BCCs shows that these miRNAs are primarily involved in various types of cancers, immune response, epithelial growth, and morphogenesis, as well as energy production and metabolism, indicating that BCC development is caused, at least in part, by changes in miRNA regulation for biological and disease processes. In particular, the “basal cell carcinoma pathways” were found to be enriched by predicted DEM targets, and regulatory relationships between DEMs and their targeted genes in this pathway were further uncovered. These results revealed the association between BCCs and abundant miRNA molecules that regulate target genes, functional modules, and signaling pathways in carcinogenesis. |
| format | Article |
| id | doaj-art-17269a0b815e49a7a521b4daaa471a8f |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-17269a0b815e49a7a521b4daaa471a8f2025-02-03T05:44:08ZengWileyJournal of Immunology Research2314-88612314-71562021-01-01202110.1155/2021/72235007223500Genome-Wide Expression Difference of MicroRNAs in Basal Cell CarcinomaHai-Peng Wei0Song Zhan1Qing-An Zhu2Zhen-Juan Chen3Xian Feng4Jun-Yuan Chen5Qi-Lin Zhang6Jingjie Zhao7Lingzhang Meng8Department of Dermatology & STD, Beihai People’s Hospital, Beihai City, Guangxi Province, ChinaDepartment of Dermatology & STD, Beihai People’s Hospital, Beihai City, Guangxi Province, ChinaDepartment of Dermatology & STD, Beihai People’s Hospital, Beihai City, Guangxi Province, ChinaDepartment of Dermatology & STD, Beihai People’s Hospital, Beihai City, Guangxi Province, ChinaDepartment of Dermatology & STD, Beihai People’s Hospital, Beihai City, Guangxi Province, ChinaState Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing City, Guangxi Province, ChinaDepartment of Dermatology & STD, Beihai People’s Hospital, Beihai City, Guangxi Province, ChinaLife Science and Clinical Research Center, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaCenter for Systemic Inflammation Research (CSIR), School of Preclinical Medicine, Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaDistinct expression of the miRNAs has rarely been explored in basal cell carcinoma (BCC) of skin, and the regulatory role of miRNAs in BCC development remains quite opaque. Here, we collected control tissues from adjacent noncancerous skin (n=15; control group) and tissues at tumor centers from patients with cheek BCC (n=15; BCC group) using punch biopsies. After six small RNA sequencing- (sRNA-seq-) based miRNA expression profiles were generated for both BCC and controls, including three biological replicates, we conducted comparative analysis on the sRNA-seq dataset, discovering 181 differentially expressed miRNAs (DEMs) out of the 1,873 miRNAs in BCCs. In order to validate the sRNA-seq data, expression of 15 randomly selected DEMs was measured using the TaqMan probe-based quantitative real-time PCR. Functional analysis of predicted target genes of DEMs in BCCs shows that these miRNAs are primarily involved in various types of cancers, immune response, epithelial growth, and morphogenesis, as well as energy production and metabolism, indicating that BCC development is caused, at least in part, by changes in miRNA regulation for biological and disease processes. In particular, the “basal cell carcinoma pathways” were found to be enriched by predicted DEM targets, and regulatory relationships between DEMs and their targeted genes in this pathway were further uncovered. These results revealed the association between BCCs and abundant miRNA molecules that regulate target genes, functional modules, and signaling pathways in carcinogenesis.http://dx.doi.org/10.1155/2021/7223500 |
| spellingShingle | Hai-Peng Wei Song Zhan Qing-An Zhu Zhen-Juan Chen Xian Feng Jun-Yuan Chen Qi-Lin Zhang Jingjie Zhao Lingzhang Meng Genome-Wide Expression Difference of MicroRNAs in Basal Cell Carcinoma Journal of Immunology Research |
| title | Genome-Wide Expression Difference of MicroRNAs in Basal Cell Carcinoma |
| title_full | Genome-Wide Expression Difference of MicroRNAs in Basal Cell Carcinoma |
| title_fullStr | Genome-Wide Expression Difference of MicroRNAs in Basal Cell Carcinoma |
| title_full_unstemmed | Genome-Wide Expression Difference of MicroRNAs in Basal Cell Carcinoma |
| title_short | Genome-Wide Expression Difference of MicroRNAs in Basal Cell Carcinoma |
| title_sort | genome wide expression difference of micrornas in basal cell carcinoma |
| url | http://dx.doi.org/10.1155/2021/7223500 |
| work_keys_str_mv | AT haipengwei genomewideexpressiondifferenceofmicrornasinbasalcellcarcinoma AT songzhan genomewideexpressiondifferenceofmicrornasinbasalcellcarcinoma AT qinganzhu genomewideexpressiondifferenceofmicrornasinbasalcellcarcinoma AT zhenjuanchen genomewideexpressiondifferenceofmicrornasinbasalcellcarcinoma AT xianfeng genomewideexpressiondifferenceofmicrornasinbasalcellcarcinoma AT junyuanchen genomewideexpressiondifferenceofmicrornasinbasalcellcarcinoma AT qilinzhang genomewideexpressiondifferenceofmicrornasinbasalcellcarcinoma AT jingjiezhao genomewideexpressiondifferenceofmicrornasinbasalcellcarcinoma AT lingzhangmeng genomewideexpressiondifferenceofmicrornasinbasalcellcarcinoma |