Attenuation and Immunogenicity of a Live High Pathogenic PRRSV Vaccine Candidate with a 32-Amino Acid Deletion in the nsp2 Protein
A porcine reproductive and respiratory syndrome virus (PRRSV) QY1 was serially passed on Marc-145 cells. Virulence of different intermediate derivatives of QY1 (P5, P60, P80, and P100) were determined. The study found that QY1 had been gradually attenuated during the in vitro process. Pathogenicity...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2014/810523 |
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| author | Wenhui Lu Baoli Sun Jianyue Mo Xiduo Zeng Guanqun Zhang Lianxiang Wang Qingfeng Zhou Ling Zhu Zhili Li Qingmei Xie Yinzuo Bi Jingyun Ma |
| author_facet | Wenhui Lu Baoli Sun Jianyue Mo Xiduo Zeng Guanqun Zhang Lianxiang Wang Qingfeng Zhou Ling Zhu Zhili Li Qingmei Xie Yinzuo Bi Jingyun Ma |
| author_sort | Wenhui Lu |
| collection | DOAJ |
| description | A porcine reproductive and respiratory syndrome virus (PRRSV) QY1 was serially passed on Marc-145 cells. Virulence of different intermediate derivatives of QY1 (P5, P60, P80, and P100) were determined. The study found that QY1 had been gradually attenuated during the in vitro process. Pathogenicity study showed that pigs inoculated with QY1 P100 and P80 did not develop any significant PRRS clinic symptoms. However, mild-to-moderate clinical signs and acute HP-PRRSV symptoms of infection were observed in pigs inoculated with QY1 P60 and P5, respectively. Furthermore, we determined the whole genome sequences of these four intermediate viruses. The results showed that after 100 passages, compared to QY1 P5, a total of 32 amino acid mutations were found. Moreover, there were one nucleotide deletion and a unique 34-amino acid deletion found at 5′UTR and in nsp2 gene during the attenuation process, respectively. Such deletions were genetically stable in vivo. Following PRRSV experimental challenge, pigs inoculated with a single dose of QY1 P100 developed no significant clinic symptoms and well tolerated lethal challenge, while QY1 P80 group still developed mild fever in the clinic trial after challenge. Thus, we concluded that QY1 P100 was a promising and highly attenuated PRRSV vaccine candidate. |
| format | Article |
| id | doaj-art-1711220d76a44dd2b8c83be396ddd937 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-1711220d76a44dd2b8c83be396ddd9372025-02-03T01:07:24ZengWileyJournal of Immunology Research2314-88612314-71562014-01-01201410.1155/2014/810523810523Attenuation and Immunogenicity of a Live High Pathogenic PRRSV Vaccine Candidate with a 32-Amino Acid Deletion in the nsp2 ProteinWenhui Lu0Baoli Sun1Jianyue Mo2Xiduo Zeng3Guanqun Zhang4Lianxiang Wang5Qingfeng Zhou6Ling Zhu7Zhili Li8Qingmei Xie9Yinzuo Bi10Jingyun Ma11College of Animal Science, South China Agricultural University, Tianhe District, Wushan Road, Guangzhou, Guangdong 510642, ChinaCollege of Animal Science, South China Agricultural University, Tianhe District, Wushan Road, Guangzhou, Guangdong 510642, ChinaCollege of Animal Science, South China Agricultural University, Tianhe District, Wushan Road, Guangzhou, Guangdong 510642, ChinaCollege of Animal Science, South China Agricultural University, Tianhe District, Wushan Road, Guangzhou, Guangdong 510642, ChinaGuangdong Wen’s Foodstuff Group Co., Ltd., Yanjiang Street, Xinxing, Guangdong 527400, ChinaGuangdong Wen’s Foodstuff Group Co., Ltd., Yanjiang Street, Xinxing, Guangdong 527400, ChinaGuangdong Wen’s Foodstuff Group Co., Ltd., Yanjiang Street, Xinxing, Guangdong 527400, ChinaCollege of Animal Science, South China Agricultural University, Tianhe District, Wushan Road, Guangzhou, Guangdong 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Tianhe District, Wushan Road, Guangzhou, Guangdong 510642, ChinaCollege of Animal Science, South China Agricultural University, Tianhe District, Wushan Road, Guangzhou, Guangdong 510642, ChinaCollege of Animal Science, South China Agricultural University, Tianhe District, Wushan Road, Guangzhou, Guangdong 510642, ChinaCollege of Animal Science, South China Agricultural University, Tianhe District, Wushan Road, Guangzhou, Guangdong 510642, ChinaA porcine reproductive and respiratory syndrome virus (PRRSV) QY1 was serially passed on Marc-145 cells. Virulence of different intermediate derivatives of QY1 (P5, P60, P80, and P100) were determined. The study found that QY1 had been gradually attenuated during the in vitro process. Pathogenicity study showed that pigs inoculated with QY1 P100 and P80 did not develop any significant PRRS clinic symptoms. However, mild-to-moderate clinical signs and acute HP-PRRSV symptoms of infection were observed in pigs inoculated with QY1 P60 and P5, respectively. Furthermore, we determined the whole genome sequences of these four intermediate viruses. The results showed that after 100 passages, compared to QY1 P5, a total of 32 amino acid mutations were found. Moreover, there were one nucleotide deletion and a unique 34-amino acid deletion found at 5′UTR and in nsp2 gene during the attenuation process, respectively. Such deletions were genetically stable in vivo. Following PRRSV experimental challenge, pigs inoculated with a single dose of QY1 P100 developed no significant clinic symptoms and well tolerated lethal challenge, while QY1 P80 group still developed mild fever in the clinic trial after challenge. Thus, we concluded that QY1 P100 was a promising and highly attenuated PRRSV vaccine candidate.http://dx.doi.org/10.1155/2014/810523 |
| spellingShingle | Wenhui Lu Baoli Sun Jianyue Mo Xiduo Zeng Guanqun Zhang Lianxiang Wang Qingfeng Zhou Ling Zhu Zhili Li Qingmei Xie Yinzuo Bi Jingyun Ma Attenuation and Immunogenicity of a Live High Pathogenic PRRSV Vaccine Candidate with a 32-Amino Acid Deletion in the nsp2 Protein Journal of Immunology Research |
| title | Attenuation and Immunogenicity of a Live High Pathogenic PRRSV Vaccine Candidate with a 32-Amino Acid Deletion in the nsp2 Protein |
| title_full | Attenuation and Immunogenicity of a Live High Pathogenic PRRSV Vaccine Candidate with a 32-Amino Acid Deletion in the nsp2 Protein |
| title_fullStr | Attenuation and Immunogenicity of a Live High Pathogenic PRRSV Vaccine Candidate with a 32-Amino Acid Deletion in the nsp2 Protein |
| title_full_unstemmed | Attenuation and Immunogenicity of a Live High Pathogenic PRRSV Vaccine Candidate with a 32-Amino Acid Deletion in the nsp2 Protein |
| title_short | Attenuation and Immunogenicity of a Live High Pathogenic PRRSV Vaccine Candidate with a 32-Amino Acid Deletion in the nsp2 Protein |
| title_sort | attenuation and immunogenicity of a live high pathogenic prrsv vaccine candidate with a 32 amino acid deletion in the nsp2 protein |
| url | http://dx.doi.org/10.1155/2014/810523 |
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