Lipopolysaccharide-induced changes in the hepatic transcriptome revealed breed-specific response in St. Croix and Suffolk sheep
This study aimed to investigate breed-specific differences in the liver transcriptomic response to lipopolysaccharide (LPS) in St. Croix (STC) and Suffolk (SUF) sheep. A total of 18 sheep (9 STC and 9 SUF) were administered LPS (E. coli O111), at a dosage of 2.5 mg/kg via I.V. After euthanasia, live...
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Animal Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fanim.2025.1565253/full |
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| author | Samanthia R. Johnson Kelsey L. Bentley Scott A. Bowdridge Scott A. Bowdridge Ibukun M. Ogunade |
| author_facet | Samanthia R. Johnson Kelsey L. Bentley Scott A. Bowdridge Scott A. Bowdridge Ibukun M. Ogunade |
| author_sort | Samanthia R. Johnson |
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| description | This study aimed to investigate breed-specific differences in the liver transcriptomic response to lipopolysaccharide (LPS) in St. Croix (STC) and Suffolk (SUF) sheep. A total of 18 sheep (9 STC and 9 SUF) were administered LPS (E. coli O111), at a dosage of 2.5 mg/kg via I.V. After euthanasia, liver samples were collected at three time points: HR0 (n = 3 per breed; no LPS), HR2 (n = 3 per breed; 2 hours after LPS), and HR6 (n = 3 per breed; 6 hours after LPS). RNA sequencing and pathway enrichment analysis were conducted to assess differentially expressed genes (DEG; FDR ≤ 0.05) and associated biological processes. At baseline (HR0), 141 DEGs were identified between STC and SUF. SUF sheep upregulated pathways related to immune readiness, including defense responses and innate immune responses. At HR2, STC had 1,719 DEGs with top upregulated genes including SELE and CCL20. Alternatively, SUF had 2,742 DEGs at HR2, with similar immune response genes expressed but at lower magnitudes. At HR6, STC had a significant shift in gene expression, with 5,568 DEGs. Notably, STC shifted from immunity-related pathways to metabolic processes. In contrast, SUF had 5,862 DEGs at HR6, continuing to express pathways related to cytokine signaling and cellular responses. Overall, STC exhibited a more intense initial response to LPS, followed by a shift towards increased differential expression of genes associated with metabolism rather than immunity. SUF, however, maintained heightened immune activity even after 6 hours, suggesting a less effective resolution of the LPS-induced challenge. |
| format | Article |
| id | doaj-art-16fe18eaed3d4f868c7d84e67b52a852 |
| institution | Kabale University |
| issn | 2673-6225 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Animal Science |
| spelling | doaj-art-16fe18eaed3d4f868c7d84e67b52a8522025-08-20T03:33:27ZengFrontiers Media S.A.Frontiers in Animal Science2673-62252025-07-01610.3389/fanim.2025.15652531565253Lipopolysaccharide-induced changes in the hepatic transcriptome revealed breed-specific response in St. Croix and Suffolk sheepSamanthia R. Johnson0Kelsey L. Bentley1Scott A. Bowdridge2Scott A. Bowdridge3Ibukun M. Ogunade4School of Agriculture and Food Systems, West Virginia University, Morgantown, WV, United StatesSchool of Agriculture and Food Systems, West Virginia University, Morgantown, WV, United StatesSchool of Agriculture and Food Systems, West Virginia University, Morgantown, WV, United StatesWest Virginia University Extension, Morgantown, WV, United StatesSchool of Agriculture and Food Systems, West Virginia University, Morgantown, WV, United StatesThis study aimed to investigate breed-specific differences in the liver transcriptomic response to lipopolysaccharide (LPS) in St. Croix (STC) and Suffolk (SUF) sheep. A total of 18 sheep (9 STC and 9 SUF) were administered LPS (E. coli O111), at a dosage of 2.5 mg/kg via I.V. After euthanasia, liver samples were collected at three time points: HR0 (n = 3 per breed; no LPS), HR2 (n = 3 per breed; 2 hours after LPS), and HR6 (n = 3 per breed; 6 hours after LPS). RNA sequencing and pathway enrichment analysis were conducted to assess differentially expressed genes (DEG; FDR ≤ 0.05) and associated biological processes. At baseline (HR0), 141 DEGs were identified between STC and SUF. SUF sheep upregulated pathways related to immune readiness, including defense responses and innate immune responses. At HR2, STC had 1,719 DEGs with top upregulated genes including SELE and CCL20. Alternatively, SUF had 2,742 DEGs at HR2, with similar immune response genes expressed but at lower magnitudes. At HR6, STC had a significant shift in gene expression, with 5,568 DEGs. Notably, STC shifted from immunity-related pathways to metabolic processes. In contrast, SUF had 5,862 DEGs at HR6, continuing to express pathways related to cytokine signaling and cellular responses. Overall, STC exhibited a more intense initial response to LPS, followed by a shift towards increased differential expression of genes associated with metabolism rather than immunity. SUF, however, maintained heightened immune activity even after 6 hours, suggesting a less effective resolution of the LPS-induced challenge.https://www.frontiersin.org/articles/10.3389/fanim.2025.1565253/fulltranscriptomeLPSinflammatoryDEGsresponse |
| spellingShingle | Samanthia R. Johnson Kelsey L. Bentley Scott A. Bowdridge Scott A. Bowdridge Ibukun M. Ogunade Lipopolysaccharide-induced changes in the hepatic transcriptome revealed breed-specific response in St. Croix and Suffolk sheep Frontiers in Animal Science transcriptome LPS inflammatory DEGs response |
| title | Lipopolysaccharide-induced changes in the hepatic transcriptome revealed breed-specific response in St. Croix and Suffolk sheep |
| title_full | Lipopolysaccharide-induced changes in the hepatic transcriptome revealed breed-specific response in St. Croix and Suffolk sheep |
| title_fullStr | Lipopolysaccharide-induced changes in the hepatic transcriptome revealed breed-specific response in St. Croix and Suffolk sheep |
| title_full_unstemmed | Lipopolysaccharide-induced changes in the hepatic transcriptome revealed breed-specific response in St. Croix and Suffolk sheep |
| title_short | Lipopolysaccharide-induced changes in the hepatic transcriptome revealed breed-specific response in St. Croix and Suffolk sheep |
| title_sort | lipopolysaccharide induced changes in the hepatic transcriptome revealed breed specific response in st croix and suffolk sheep |
| topic | transcriptome LPS inflammatory DEGs response |
| url | https://www.frontiersin.org/articles/10.3389/fanim.2025.1565253/full |
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