Extraction, Chemical Composition and Antidiabetic Potential of Crude Polysaccharides from Centella asiatica (L.) Urban
The global prevalence of diabetes mellitus (DM) is escalating, posing a significant challenge to human healthcare systems. In the present study, mineral and monosaccharide compositions, and antidiabetic potential of polysaccharide fractions separated from Centella asiatica (L.) leaves (CAL) were a...
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Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
2024-05-01
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| Series: | Polish Journal of Food and Nutrition Sciences |
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| Online Access: | http://journal.pan.olsztyn.pl/Extraction-Chemical-Composition-and-Antidiabetic-Potential-of-Crude-Polysaccharides,188316,0,2.html |
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| author | Meng Li Muhammad Shahid Xiaobin Zhang Douglas Law Mukram Muhammad Mackeen Arnida Hani Teh Ahmad Abdul Kareem Najm Shazrul Fazry Babul Airianah Othman |
| author_facet | Meng Li Muhammad Shahid Xiaobin Zhang Douglas Law Mukram Muhammad Mackeen Arnida Hani Teh Ahmad Abdul Kareem Najm Shazrul Fazry Babul Airianah Othman |
| author_sort | Meng Li |
| collection | DOAJ |
| description | The global prevalence of diabetes mellitus (DM) is escalating, posing a significant challenge to human healthcare systems. In the present study, mineral and monosaccharide compositions, and antidiabetic potential of polysaccharide fractions separated from Centella asiatica (L.) leaves (CAL) were assessed. Initial single-factor experiments identified key extraction parameters, further optimized through response surface methodology (RSM) with optimal conditions determined as a liquid-to-solid ratio of 24.43:1 (mL/g), extraction time of 60.76 min, and a temperature of 83.31℃. Alcohol insoluble residue (AIR) extraction yielded three crude polysaccharide fractions (P50, P70, and P90). P50 was obtained with the highest yield (14.31%) and exhibited the highest content of total sugars and uronic acids (68.01 and 24.28 g/100 g, respectively). Calcium, magnesium, and manganese were dominant minerals in P50 and P70. Monosaccharide composition analysis via high-performance liquid chromatography (HPLC) demonstrated enrichment of galacturonic acid, galactose, and arabinose in P50 and P70. Furthermore, both fractions (P50 and P70) exhibited dose-dependent inhibitory effects on α-amylase and α-glucosidase. At 5 mg/mL, P50 caused 68.3% α-amylase inhibition and 62.3% α-glucosidase inhibition, while P70 triggered 46.8% and 34.1% inhibition, respectively. In conclusion, this study is the first to provide valuable insights into optimizing CAL extraction conditions. Potential utilization of polysaccharide fractions obtained from CAL as components for formulating functional foods can be explored in the future. |
| format | Article |
| id | doaj-art-16e2758980324d63af24cc2d11b6d9fd |
| institution | Kabale University |
| issn | 2083-6007 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences |
| record_format | Article |
| series | Polish Journal of Food and Nutrition Sciences |
| spelling | doaj-art-16e2758980324d63af24cc2d11b6d9fd2025-02-02T06:04:56ZengInstitute of Animal Reproduction and Food Research of the Polish Academy of SciencesPolish Journal of Food and Nutrition Sciences2083-60072024-05-0174217718710.31883/pjfns/188316188316Extraction, Chemical Composition and Antidiabetic Potential of Crude Polysaccharides from Centella asiatica (L.) UrbanMeng Li0https://orcid.org/0000-0001-5862-3778Muhammad Shahid1Xiaobin Zhang2Douglas Law3https://orcid.org/0000-0002-7824-3542Mukram Muhammad Mackeen4Arnida Hani Teh5Ahmad Abdul Kareem Najm6Shazrul Fazry7https://orcid.org/0000-0002-7072-8609Babul Airianah Othman8https://orcid.org/0000-0003-3250-9648Department of Biological Sciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600, MalaysiaDepartment of Biological Sciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600, MalaysiaCollege of Horticulture, Hainan University, Haikou, Hainan 570228, ChinaFaculty of Health and Life Sciences, INTI International University, Persiaran Perdana BBN Putra Nilai, Nilai 71800, MalaysiaDepartment of Chemistry, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600, MalaysiaDepartment of Food Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600, MalaysiaDepartment of Food Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600, MalaysiaDepartment of Food Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600, MalaysiaDepartment of Food Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600, MalaysiaThe global prevalence of diabetes mellitus (DM) is escalating, posing a significant challenge to human healthcare systems. In the present study, mineral and monosaccharide compositions, and antidiabetic potential of polysaccharide fractions separated from Centella asiatica (L.) leaves (CAL) were assessed. Initial single-factor experiments identified key extraction parameters, further optimized through response surface methodology (RSM) with optimal conditions determined as a liquid-to-solid ratio of 24.43:1 (mL/g), extraction time of 60.76 min, and a temperature of 83.31℃. Alcohol insoluble residue (AIR) extraction yielded three crude polysaccharide fractions (P50, P70, and P90). P50 was obtained with the highest yield (14.31%) and exhibited the highest content of total sugars and uronic acids (68.01 and 24.28 g/100 g, respectively). Calcium, magnesium, and manganese were dominant minerals in P50 and P70. Monosaccharide composition analysis via high-performance liquid chromatography (HPLC) demonstrated enrichment of galacturonic acid, galactose, and arabinose in P50 and P70. Furthermore, both fractions (P50 and P70) exhibited dose-dependent inhibitory effects on α-amylase and α-glucosidase. At 5 mg/mL, P50 caused 68.3% α-amylase inhibition and 62.3% α-glucosidase inhibition, while P70 triggered 46.8% and 34.1% inhibition, respectively. In conclusion, this study is the first to provide valuable insights into optimizing CAL extraction conditions. Potential utilization of polysaccharide fractions obtained from CAL as components for formulating functional foods can be explored in the future.http://journal.pan.olsztyn.pl/Extraction-Chemical-Composition-and-Antidiabetic-Potential-of-Crude-Polysaccharides,188316,0,2.htmlalcohol insoluble residueantidiabeticmineral elementsmonosaccharide compositionresponse surface methodology |
| spellingShingle | Meng Li Muhammad Shahid Xiaobin Zhang Douglas Law Mukram Muhammad Mackeen Arnida Hani Teh Ahmad Abdul Kareem Najm Shazrul Fazry Babul Airianah Othman Extraction, Chemical Composition and Antidiabetic Potential of Crude Polysaccharides from Centella asiatica (L.) Urban Polish Journal of Food and Nutrition Sciences alcohol insoluble residue antidiabetic mineral elements monosaccharide composition response surface methodology |
| title | Extraction, Chemical Composition and Antidiabetic Potential of Crude Polysaccharides from Centella asiatica (L.) Urban |
| title_full | Extraction, Chemical Composition and Antidiabetic Potential of Crude Polysaccharides from Centella asiatica (L.) Urban |
| title_fullStr | Extraction, Chemical Composition and Antidiabetic Potential of Crude Polysaccharides from Centella asiatica (L.) Urban |
| title_full_unstemmed | Extraction, Chemical Composition and Antidiabetic Potential of Crude Polysaccharides from Centella asiatica (L.) Urban |
| title_short | Extraction, Chemical Composition and Antidiabetic Potential of Crude Polysaccharides from Centella asiatica (L.) Urban |
| title_sort | extraction chemical composition and antidiabetic potential of crude polysaccharides from centella asiatica l urban |
| topic | alcohol insoluble residue antidiabetic mineral elements monosaccharide composition response surface methodology |
| url | http://journal.pan.olsztyn.pl/Extraction-Chemical-Composition-and-Antidiabetic-Potential-of-Crude-Polysaccharides,188316,0,2.html |
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