Cell Imaging by Spontaneous and Amplified Raman Spectroscopies
Raman spectroscopy (RS) is a powerful, noninvasive optical technique able to detect vibrational modes of chemical bonds. The high chemical specificity due to its fingerprinting character and the minimal requests for sample preparation have rendered it nowadays very popular in the analysis of biosyst...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Spectroscopy |
| Online Access: | http://dx.doi.org/10.1155/2017/2193656 |
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| author | Giulia Rusciano Gianluigi Zito Giuseppe Pesce Antonio Sasso |
| author_facet | Giulia Rusciano Gianluigi Zito Giuseppe Pesce Antonio Sasso |
| author_sort | Giulia Rusciano |
| collection | DOAJ |
| description | Raman spectroscopy (RS) is a powerful, noninvasive optical technique able to detect vibrational modes of chemical bonds. The high chemical specificity due to its fingerprinting character and the minimal requests for sample preparation have rendered it nowadays very popular in the analysis of biosystems for diagnostic purposes. In this paper, we first discuss the main advantages of spontaneous RS by describing the study of a single protozoan (Acanthamoeba), which plays an important role in a severe ophthalmological disease (Acanthamoeba keratitis). Later on, we point out that the weak signals that originated from Raman scattering do not allow probing optically thin samples, such as cellular membrane. Experimental approaches able to overcome this drawback are based on the use of metallic nanostructures, which lead to a huge amplification of the Raman yields thanks to the excitation of localized surface plasmon resonances. Surface-enhanced Raman scattering (SERS) and tip-enhanced Raman scattering (TERS) are examples of such innovative techniques, in which metallic nanostructures are assembled on a flat surface or on the tip of a scanning probe microscope, respectively. Herein, we provide a couple of examples (red blood cells and bacterial spores) aimed at studying cell membranes with these techniques. |
| format | Article |
| id | doaj-art-16d5b16d20294083943feb4e8a147221 |
| institution | Kabale University |
| issn | 2314-4920 2314-4939 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Spectroscopy |
| spelling | doaj-art-16d5b16d20294083943feb4e8a1472212025-02-03T00:59:48ZengWileyJournal of Spectroscopy2314-49202314-49392017-01-01201710.1155/2017/21936562193656Cell Imaging by Spontaneous and Amplified Raman SpectroscopiesGiulia Rusciano0Gianluigi Zito1Giuseppe Pesce2Antonio Sasso3Department of Physics “E. Pancini”, University of Naples Federico II, Via Cintia, 80126 Naples, ItalyInstitute of Protein Biochemistry (IBP), National Research Council (CNR), Via Pietro Castellino 111, 80131 Napoli, ItalyDepartment of Physics “E. Pancini”, University of Naples Federico II, Via Cintia, 80126 Naples, ItalyDepartment of Physics “E. Pancini”, University of Naples Federico II, Via Cintia, 80126 Naples, ItalyRaman spectroscopy (RS) is a powerful, noninvasive optical technique able to detect vibrational modes of chemical bonds. The high chemical specificity due to its fingerprinting character and the minimal requests for sample preparation have rendered it nowadays very popular in the analysis of biosystems for diagnostic purposes. In this paper, we first discuss the main advantages of spontaneous RS by describing the study of a single protozoan (Acanthamoeba), which plays an important role in a severe ophthalmological disease (Acanthamoeba keratitis). Later on, we point out that the weak signals that originated from Raman scattering do not allow probing optically thin samples, such as cellular membrane. Experimental approaches able to overcome this drawback are based on the use of metallic nanostructures, which lead to a huge amplification of the Raman yields thanks to the excitation of localized surface plasmon resonances. Surface-enhanced Raman scattering (SERS) and tip-enhanced Raman scattering (TERS) are examples of such innovative techniques, in which metallic nanostructures are assembled on a flat surface or on the tip of a scanning probe microscope, respectively. Herein, we provide a couple of examples (red blood cells and bacterial spores) aimed at studying cell membranes with these techniques.http://dx.doi.org/10.1155/2017/2193656 |
| spellingShingle | Giulia Rusciano Gianluigi Zito Giuseppe Pesce Antonio Sasso Cell Imaging by Spontaneous and Amplified Raman Spectroscopies Journal of Spectroscopy |
| title | Cell Imaging by Spontaneous and Amplified Raman Spectroscopies |
| title_full | Cell Imaging by Spontaneous and Amplified Raman Spectroscopies |
| title_fullStr | Cell Imaging by Spontaneous and Amplified Raman Spectroscopies |
| title_full_unstemmed | Cell Imaging by Spontaneous and Amplified Raman Spectroscopies |
| title_short | Cell Imaging by Spontaneous and Amplified Raman Spectroscopies |
| title_sort | cell imaging by spontaneous and amplified raman spectroscopies |
| url | http://dx.doi.org/10.1155/2017/2193656 |
| work_keys_str_mv | AT giuliarusciano cellimagingbyspontaneousandamplifiedramanspectroscopies AT gianluigizito cellimagingbyspontaneousandamplifiedramanspectroscopies AT giuseppepesce cellimagingbyspontaneousandamplifiedramanspectroscopies AT antoniosasso cellimagingbyspontaneousandamplifiedramanspectroscopies |