Insights of cellular and molecular changes in sugarcane response to oxidative signaling
Abstract Main conclusion Significant changes in the proteome highlight essential metabolic adaptations for development and oxidative signaling induced by the treatment of young sugarcane plants with hydrogen peroxide. These adaptations suggest that hydrogen peroxide acts not only as a stressor but p...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-01-01
|
| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-024-06036-x |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832594811018805248 |
|---|---|
| author | Lucas Felipe Silva Kellya F. M. Barreto Helaine Cristiane Silva Iara Dantas de Souza Carlos H. S. G. Meneses Adriana F. Uchôa Katia C. Scortecci |
| author_facet | Lucas Felipe Silva Kellya F. M. Barreto Helaine Cristiane Silva Iara Dantas de Souza Carlos H. S. G. Meneses Adriana F. Uchôa Katia C. Scortecci |
| author_sort | Lucas Felipe Silva |
| collection | DOAJ |
| description | Abstract Main conclusion Significant changes in the proteome highlight essential metabolic adaptations for development and oxidative signaling induced by the treatment of young sugarcane plants with hydrogen peroxide. These adaptations suggest that hydrogen peroxide acts not only as a stressor but primarily as a signaling molecule, triggering specific metabolic pathways that regulate growth and plant resilience. Abstract Sugarcane is a crucial crop for sugar and ethanol production, often influenced by environmental signals. Hydrogen peroxide (H2O2) is increasingly recognized as an important signaling molecule that regulates plant development and adaptation. In this study, two-month-old sugarcane plants were treated with varying concentrations of H2O2 to investigate how this molecule acts as a signal at the cellular, biochemical, and proteomic levels. Antioxidant enzyme activity exhibited fluctuations, suggesting a dynamic response to oxidative signaling. Lipid peroxidation, observed through TBARs and scanning electron microscopy, highlighted early membrane modifications. Proteomic analysis (ProteomeXchange PXD048142) identified 2,699 proteins, with 155 showing significant expression changes in response to H2O2 signaling. Bioinformatics, including Principal Component Analysis, revealed distinct proteomic profiles in roots and leaves, indicating tissue-specific metabolic reprogramming. Functional annotation through Gene Ontology and KEGG pathway enrichment showed that oxidative signaling led to the repression of photosynthesis-related pathways in leaves, while promoting pathways related to protein processing, glycolysis, and carbon metabolism in roots. Additionally, bioinformatic tools identified proteins involved in amino acid metabolism, the TCA cycle, and carbohydrate metabolism as critical components of sugarcane's adaptive signaling response. The data suggest that sugarcane plants responded to oxidative signals by adjusting their metabolic networks, promoting sustained development and potential pathways for targeted plant breeding. |
| format | Article |
| id | doaj-art-16c9be34978c483c96de5e77e58c85ef |
| institution | Kabale University |
| issn | 1471-2229 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj-art-16c9be34978c483c96de5e77e58c85ef2025-01-19T12:16:51ZengBMCBMC Plant Biology1471-22292025-01-0125112610.1186/s12870-024-06036-xInsights of cellular and molecular changes in sugarcane response to oxidative signalingLucas Felipe Silva0Kellya F. M. Barreto1Helaine Cristiane Silva2Iara Dantas de Souza3Carlos H. S. G. Meneses4Adriana F. Uchôa5Katia C. Scortecci6Bioinformatics Multidisciplinary Environment, IMD, Universidade Federal Do Rio Grande Do NorteLPTAM, Universidade Federal Do Rio Grande Do NorteLPTAM, Universidade Federal Do Rio Grande Do NorteBioinformatics Multidisciplinary Environment, IMD, Universidade Federal Do Rio Grande Do NortePlant Biotechnology Laboratory, Department of Biology – Centro de Ciências Biológicas e da Saúde, , Universidade Estadual da ParaíbaBiochemistry and Molecular Biology Graduate School, Centro de Biociências, Universidade Federal Do Rio Grande Do NorteBioinformatics Multidisciplinary Environment, IMD, Universidade Federal Do Rio Grande Do NorteAbstract Main conclusion Significant changes in the proteome highlight essential metabolic adaptations for development and oxidative signaling induced by the treatment of young sugarcane plants with hydrogen peroxide. These adaptations suggest that hydrogen peroxide acts not only as a stressor but primarily as a signaling molecule, triggering specific metabolic pathways that regulate growth and plant resilience. Abstract Sugarcane is a crucial crop for sugar and ethanol production, often influenced by environmental signals. Hydrogen peroxide (H2O2) is increasingly recognized as an important signaling molecule that regulates plant development and adaptation. In this study, two-month-old sugarcane plants were treated with varying concentrations of H2O2 to investigate how this molecule acts as a signal at the cellular, biochemical, and proteomic levels. Antioxidant enzyme activity exhibited fluctuations, suggesting a dynamic response to oxidative signaling. Lipid peroxidation, observed through TBARs and scanning electron microscopy, highlighted early membrane modifications. Proteomic analysis (ProteomeXchange PXD048142) identified 2,699 proteins, with 155 showing significant expression changes in response to H2O2 signaling. Bioinformatics, including Principal Component Analysis, revealed distinct proteomic profiles in roots and leaves, indicating tissue-specific metabolic reprogramming. Functional annotation through Gene Ontology and KEGG pathway enrichment showed that oxidative signaling led to the repression of photosynthesis-related pathways in leaves, while promoting pathways related to protein processing, glycolysis, and carbon metabolism in roots. Additionally, bioinformatic tools identified proteins involved in amino acid metabolism, the TCA cycle, and carbohydrate metabolism as critical components of sugarcane's adaptive signaling response. The data suggest that sugarcane plants responded to oxidative signals by adjusting their metabolic networks, promoting sustained development and potential pathways for targeted plant breeding.https://doi.org/10.1186/s12870-024-06036-xSaccharum spp.MicroscopyAntioxidant enzymesH2O2ProteomicsBioinformatics |
| spellingShingle | Lucas Felipe Silva Kellya F. M. Barreto Helaine Cristiane Silva Iara Dantas de Souza Carlos H. S. G. Meneses Adriana F. Uchôa Katia C. Scortecci Insights of cellular and molecular changes in sugarcane response to oxidative signaling BMC Plant Biology Saccharum spp. Microscopy Antioxidant enzymes H2O2 Proteomics Bioinformatics |
| title | Insights of cellular and molecular changes in sugarcane response to oxidative signaling |
| title_full | Insights of cellular and molecular changes in sugarcane response to oxidative signaling |
| title_fullStr | Insights of cellular and molecular changes in sugarcane response to oxidative signaling |
| title_full_unstemmed | Insights of cellular and molecular changes in sugarcane response to oxidative signaling |
| title_short | Insights of cellular and molecular changes in sugarcane response to oxidative signaling |
| title_sort | insights of cellular and molecular changes in sugarcane response to oxidative signaling |
| topic | Saccharum spp. Microscopy Antioxidant enzymes H2O2 Proteomics Bioinformatics |
| url | https://doi.org/10.1186/s12870-024-06036-x |
| work_keys_str_mv | AT lucasfelipesilva insightsofcellularandmolecularchangesinsugarcaneresponsetooxidativesignaling AT kellyafmbarreto insightsofcellularandmolecularchangesinsugarcaneresponsetooxidativesignaling AT helainecristianesilva insightsofcellularandmolecularchangesinsugarcaneresponsetooxidativesignaling AT iaradantasdesouza insightsofcellularandmolecularchangesinsugarcaneresponsetooxidativesignaling AT carloshsgmeneses insightsofcellularandmolecularchangesinsugarcaneresponsetooxidativesignaling AT adrianafuchoa insightsofcellularandmolecularchangesinsugarcaneresponsetooxidativesignaling AT katiacscortecci insightsofcellularandmolecularchangesinsugarcaneresponsetooxidativesignaling |