Canine Cutaneous Leishmaniasis: Dissemination and Tissue Tropism of Genetically Distinct Leishmania (Viannia) braziliensis Populations
Little is known regarding the internal dissemination of initial cutaneous lesions and tissue tropism of Leishmania (Viannia) braziliensis populations in naturally infected dogs. The aim of this study was to investigate genetic polymorphisms of L. (V.) braziliensis populations in different anatomic s...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.1155/2013/982183 |
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| author | Guilherme Marx de Oliveira Maria de Fátima Madeira Fernanda Santos Oliveira Marize Quinhones Pires Raquel da Silva Pacheco |
| author_facet | Guilherme Marx de Oliveira Maria de Fátima Madeira Fernanda Santos Oliveira Marize Quinhones Pires Raquel da Silva Pacheco |
| author_sort | Guilherme Marx de Oliveira |
| collection | DOAJ |
| description | Little is known regarding the internal dissemination of initial cutaneous lesions and tissue tropism of Leishmania (Viannia) braziliensis populations in naturally infected dogs. The aim of this study was to investigate genetic polymorphisms of L. (V.) braziliensis populations in different anatomic sites of naturally infected dogs by using polymerase chain reaction (PCR) and low-stringency single specific primer-PCR (LSSP-PCR) techniques. The amplified products were analyzed by LSSP-PCR to investigate the genetic variability of the parasite populations present in different anatomical sites. Twenty-three out of the 52 samples gave PCR-positive results. The existence of L. (V.) braziliensis strains that remained restricted to cutaneous lesions and others showing characteristics of dissemination to internal organs and healthy skin was observed. LSSP-PCR and numerical analyses revealed that parasite populations that do not disseminate were genetically similar and belonged to a separate phenetic cluster. In contrast, populations that showed spreading to internal organs displayed a more polymorphic genetic profile. Despite the heterogeneity, L. (V.) braziliensis populations with identical genetic profiles were observed in popliteal and cervical lymph nodes of the same animal. Our results indicate that infection in dogs can be manifested by dissemination and tissue tropism of genetically distinct populations of L. (V.) braziliensis. |
| format | Article |
| id | doaj-art-16c7bf461cd6442cb47a623fd88fb04e |
| institution | Kabale University |
| issn | 2090-8113 2042-0048 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-16c7bf461cd6442cb47a623fd88fb04e2025-02-03T01:09:37ZengWileyVeterinary Medicine International2090-81132042-00482013-01-01201310.1155/2013/982183982183Canine Cutaneous Leishmaniasis: Dissemination and Tissue Tropism of Genetically Distinct Leishmania (Viannia) braziliensis PopulationsGuilherme Marx de Oliveira0Maria de Fátima Madeira1Fernanda Santos Oliveira2Marize Quinhones Pires3Raquel da Silva Pacheco4Laboratório de Epidemiologia e Sistemática Molecular, Instituto Oswaldo Cruz, FIOCRUZ, Avenida Brasil No. 4365, Manguinhos, 21040-360 Rio de Janeiro, RJ, BrazilLaboratório de Vigilância em Leishmanioses, Instituto de Pesquisa Clínica Evandro Chagas, FIOCRUZ, Avenida Brasil No. 4365, Manguinhos, 21040-360 Rio de Janeiro, RJ, BrazilLaboratório de Epidemiologia e Sistemática Molecular, Instituto Oswaldo Cruz, FIOCRUZ, Avenida Brasil No. 4365, Manguinhos, 21040-360 Rio de Janeiro, RJ, BrazilLaboratório de Epidemiologia e Sistemática Molecular, Instituto Oswaldo Cruz, FIOCRUZ, Avenida Brasil No. 4365, Manguinhos, 21040-360 Rio de Janeiro, RJ, BrazilLaboratório de Epidemiologia e Sistemática Molecular, Instituto Oswaldo Cruz, FIOCRUZ, Avenida Brasil No. 4365, Manguinhos, 21040-360 Rio de Janeiro, RJ, BrazilLittle is known regarding the internal dissemination of initial cutaneous lesions and tissue tropism of Leishmania (Viannia) braziliensis populations in naturally infected dogs. The aim of this study was to investigate genetic polymorphisms of L. (V.) braziliensis populations in different anatomic sites of naturally infected dogs by using polymerase chain reaction (PCR) and low-stringency single specific primer-PCR (LSSP-PCR) techniques. The amplified products were analyzed by LSSP-PCR to investigate the genetic variability of the parasite populations present in different anatomical sites. Twenty-three out of the 52 samples gave PCR-positive results. The existence of L. (V.) braziliensis strains that remained restricted to cutaneous lesions and others showing characteristics of dissemination to internal organs and healthy skin was observed. LSSP-PCR and numerical analyses revealed that parasite populations that do not disseminate were genetically similar and belonged to a separate phenetic cluster. In contrast, populations that showed spreading to internal organs displayed a more polymorphic genetic profile. Despite the heterogeneity, L. (V.) braziliensis populations with identical genetic profiles were observed in popliteal and cervical lymph nodes of the same animal. Our results indicate that infection in dogs can be manifested by dissemination and tissue tropism of genetically distinct populations of L. (V.) braziliensis.http://dx.doi.org/10.1155/2013/982183 |
| spellingShingle | Guilherme Marx de Oliveira Maria de Fátima Madeira Fernanda Santos Oliveira Marize Quinhones Pires Raquel da Silva Pacheco Canine Cutaneous Leishmaniasis: Dissemination and Tissue Tropism of Genetically Distinct Leishmania (Viannia) braziliensis Populations Veterinary Medicine International |
| title | Canine Cutaneous Leishmaniasis: Dissemination and Tissue Tropism of Genetically Distinct Leishmania (Viannia) braziliensis Populations |
| title_full | Canine Cutaneous Leishmaniasis: Dissemination and Tissue Tropism of Genetically Distinct Leishmania (Viannia) braziliensis Populations |
| title_fullStr | Canine Cutaneous Leishmaniasis: Dissemination and Tissue Tropism of Genetically Distinct Leishmania (Viannia) braziliensis Populations |
| title_full_unstemmed | Canine Cutaneous Leishmaniasis: Dissemination and Tissue Tropism of Genetically Distinct Leishmania (Viannia) braziliensis Populations |
| title_short | Canine Cutaneous Leishmaniasis: Dissemination and Tissue Tropism of Genetically Distinct Leishmania (Viannia) braziliensis Populations |
| title_sort | canine cutaneous leishmaniasis dissemination and tissue tropism of genetically distinct leishmania viannia braziliensis populations |
| url | http://dx.doi.org/10.1155/2013/982183 |
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