Biopsy of Different Oral Soft Tissues Lesions by KTP and Diode Laser: Histological Evaluation
Introduction. Oral biopsy aims to obtain clear and safe diagnosis; it can be performed by scalpel or laser. The controversy in this latter application is the thermal alteration due to tissue heating. The aim of this study is the histological evaluation of margins of “in vivo” biopsies collected by d...
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/761704 |
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| author | Umberto Romeo Claudia Russo Gaspare Palaia Rossella Lo Giudice Alessandro Del Vecchio Paolo Visca Guido Migliau Alberto De Biase |
| author_facet | Umberto Romeo Claudia Russo Gaspare Palaia Rossella Lo Giudice Alessandro Del Vecchio Paolo Visca Guido Migliau Alberto De Biase |
| author_sort | Umberto Romeo |
| collection | DOAJ |
| description | Introduction. Oral biopsy aims to obtain clear and safe diagnosis; it can be performed by scalpel or laser. The controversy in this latter application is the thermal alteration due to tissue heating. The aim of this study is the histological evaluation of margins of “in vivo” biopsies collected by diode and KTP lasers. Material and Methods. 17 oral benign lesions biopsies were made by diode 808 nm (SOL, DenMatItalia, Italy) and KTP 532 nm (SmartLite, DEKA, Italy). Samples were observed at OM LEICA DM 2000; margin alterations were evaluated through Leica Application Suite 3.4. Results. Epithelial and connective damages were assessed for each pathology with an average of 0.245 mm and a standard deviation of ±0.162 mm in mucoceles, 0.382 mm ± 0.149 mm in fibromas, 0.336 mm ± 0.106 mm in hyperkeratosis, 0.473 mm ± 0.105 mm in squamous hyperplasia, 0.182 mm in giant cell granuloma, and 0.149 mm in melanotic macula. Discussion. The histologic aspect of lesions influenced the response to laser, whereas the greater inflammation and cellularity were linked with the higher thermal signs. Many artifacts were also associated to histologic procedures. Conclusion. Both tested lasers permitted sure histologic diagnosis. However, it is suggested to enlarge biopsies of about 0.5 mm, to avoid thermal alterations, especially in inflammatory lesions like oral lichen planus. |
| format | Article |
| id | doaj-art-16b4ccb1a4d54dca9b245f8354dcd123 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
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| series | The Scientific World Journal |
| spelling | doaj-art-16b4ccb1a4d54dca9b245f8354dcd1232025-02-03T05:43:41ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/761704761704Biopsy of Different Oral Soft Tissues Lesions by KTP and Diode Laser: Histological EvaluationUmberto Romeo0Claudia Russo1Gaspare Palaia2Rossella Lo Giudice3Alessandro Del Vecchio4Paolo Visca5Guido Migliau6Alberto De Biase7Department of Oral and Maxillofacial Sciences, “Sapienza” University of Rome, Via Caserta 6, 00161 Rome, ItalyDepartment of Oral and Maxillofacial Sciences, “Sapienza” University of Rome, Via Caserta 6, 00161 Rome, ItalyDepartment of Oral and Maxillofacial Sciences, “Sapienza” University of Rome, Via Caserta 6, 00161 Rome, ItalyDepartment of Oral and Maxillofacial Sciences, “Sapienza” University of Rome, Via Caserta 6, 00161 Rome, ItalyDepartment of Oral and Maxillofacial Sciences, “Sapienza” University of Rome, Via Caserta 6, 00161 Rome, ItalyDepartment of Cytology and Cellular Diagnostics, Regina Elena Institute, Via Elio Chianesi 53, 00144 Rome, ItalyDepartment of Oral and Maxillofacial Sciences, “Sapienza” University of Rome, Via Caserta 6, 00161 Rome, ItalyDepartment of Oral and Maxillofacial Sciences, “Sapienza” University of Rome, Via Caserta 6, 00161 Rome, ItalyIntroduction. Oral biopsy aims to obtain clear and safe diagnosis; it can be performed by scalpel or laser. The controversy in this latter application is the thermal alteration due to tissue heating. The aim of this study is the histological evaluation of margins of “in vivo” biopsies collected by diode and KTP lasers. Material and Methods. 17 oral benign lesions biopsies were made by diode 808 nm (SOL, DenMatItalia, Italy) and KTP 532 nm (SmartLite, DEKA, Italy). Samples were observed at OM LEICA DM 2000; margin alterations were evaluated through Leica Application Suite 3.4. Results. Epithelial and connective damages were assessed for each pathology with an average of 0.245 mm and a standard deviation of ±0.162 mm in mucoceles, 0.382 mm ± 0.149 mm in fibromas, 0.336 mm ± 0.106 mm in hyperkeratosis, 0.473 mm ± 0.105 mm in squamous hyperplasia, 0.182 mm in giant cell granuloma, and 0.149 mm in melanotic macula. Discussion. The histologic aspect of lesions influenced the response to laser, whereas the greater inflammation and cellularity were linked with the higher thermal signs. Many artifacts were also associated to histologic procedures. Conclusion. Both tested lasers permitted sure histologic diagnosis. However, it is suggested to enlarge biopsies of about 0.5 mm, to avoid thermal alterations, especially in inflammatory lesions like oral lichen planus.http://dx.doi.org/10.1155/2014/761704 |
| spellingShingle | Umberto Romeo Claudia Russo Gaspare Palaia Rossella Lo Giudice Alessandro Del Vecchio Paolo Visca Guido Migliau Alberto De Biase Biopsy of Different Oral Soft Tissues Lesions by KTP and Diode Laser: Histological Evaluation The Scientific World Journal |
| title | Biopsy of Different Oral Soft Tissues Lesions by KTP and Diode Laser: Histological Evaluation |
| title_full | Biopsy of Different Oral Soft Tissues Lesions by KTP and Diode Laser: Histological Evaluation |
| title_fullStr | Biopsy of Different Oral Soft Tissues Lesions by KTP and Diode Laser: Histological Evaluation |
| title_full_unstemmed | Biopsy of Different Oral Soft Tissues Lesions by KTP and Diode Laser: Histological Evaluation |
| title_short | Biopsy of Different Oral Soft Tissues Lesions by KTP and Diode Laser: Histological Evaluation |
| title_sort | biopsy of different oral soft tissues lesions by ktp and diode laser histological evaluation |
| url | http://dx.doi.org/10.1155/2014/761704 |
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