Modification of non-selective-solid media for aquatic bacteria
ABSTRACT The objective of this research was to produce an alternative media for aquatic bacteria using fish broth as pepton source and commercial consumption agar as material. This experiment consisted of six treatments; four treatments used fish broth with doses 200 g/L; 400 g/L; 600 g/L and 800...
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Format: | Article |
Language: | English |
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Asosiasi Sains Akuakultur Indonesia
2014-07-01
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Series: | Jurnal Akuakultur Indonesia |
Online Access: | https://journal.ipb.ac.id/index.php/jai/article/view/10317 |
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author | Sefti Heza Dwinanti , Tanbiyaskur |
author_facet | Sefti Heza Dwinanti , Tanbiyaskur |
author_sort | Sefti Heza Dwinanti |
collection | DOAJ |
description | ABSTRACT
The objective of this research was to produce an alternative media for aquatic bacteria using fish broth as pepton source and commercial consumption agar as material. This experiment consisted of six treatments; four treatments used fish broth with doses 200 g/L; 400 g/L; 600 g/L and 800 g/L; two treatments as controls which were commercial agar as negative control and tryptic soy agar (TSA) as positive control. The result showed that treatment 200 g/L had performed as good as TSA for bacterial growth.
Keywords: solid media, fish broth, aquatic bacteria
ABSTRAK
Tujuan dari penelitian ini adalah menciptakan alternatif media padat nonselektif untuk bakteri akuatik dengan memanfaatkan kaldu daging ikan sebagai sumber pepton dan agar-agar konsumsi komersial (AKK) sebagai pemadat. Penelitian ini terdiri atas enam perlakuan yaitu; empat perlakuan menggunakan kaldu ikan yang dibuat dengan dosis 200 g/L; 400 g/L; 600 g/L and 800 g/L; dua perlakuan sebagai kontrol yaitu ART sebagai kontrol negatif dan tryptic soy agar (TSA) sebagai kontrol positif. Hasil penelitian menunjukkan bahwa pemanfaatan kaldu daging ikan pada dosis 200 g/L memiliki performa yang sama baiknya dengan TSA sebagai media tumbuh bakteri.
Kata kunci: media padat, kaldu ikan, bakteri akuatik
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format | Article |
id | doaj-art-16a27870e00645048c5b2a0793c2f226 |
institution | Kabale University |
issn | 1412-5269 2354-6700 |
language | English |
publishDate | 2014-07-01 |
publisher | Asosiasi Sains Akuakultur Indonesia |
record_format | Article |
series | Jurnal Akuakultur Indonesia |
spelling | doaj-art-16a27870e00645048c5b2a0793c2f2262025-01-24T17:59:51ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002014-07-0113210.19027/jai.13.163-1668500Modification of non-selective-solid media for aquatic bacteriaSefti Heza Dwinanti0, Tanbiyaskur1Program Studi Budidaya Perairan - Fakultas Pertanian Universitas SriwijayaProgram Studi Budidaya Perairan - Fakultas Pertanian Universitas SriwijayaABSTRACT The objective of this research was to produce an alternative media for aquatic bacteria using fish broth as pepton source and commercial consumption agar as material. This experiment consisted of six treatments; four treatments used fish broth with doses 200 g/L; 400 g/L; 600 g/L and 800 g/L; two treatments as controls which were commercial agar as negative control and tryptic soy agar (TSA) as positive control. The result showed that treatment 200 g/L had performed as good as TSA for bacterial growth. Keywords: solid media, fish broth, aquatic bacteria ABSTRAK Tujuan dari penelitian ini adalah menciptakan alternatif media padat nonselektif untuk bakteri akuatik dengan memanfaatkan kaldu daging ikan sebagai sumber pepton dan agar-agar konsumsi komersial (AKK) sebagai pemadat. Penelitian ini terdiri atas enam perlakuan yaitu; empat perlakuan menggunakan kaldu ikan yang dibuat dengan dosis 200 g/L; 400 g/L; 600 g/L and 800 g/L; dua perlakuan sebagai kontrol yaitu ART sebagai kontrol negatif dan tryptic soy agar (TSA) sebagai kontrol positif. Hasil penelitian menunjukkan bahwa pemanfaatan kaldu daging ikan pada dosis 200 g/L memiliki performa yang sama baiknya dengan TSA sebagai media tumbuh bakteri. Kata kunci: media padat, kaldu ikan, bakteri akuatik https://journal.ipb.ac.id/index.php/jai/article/view/10317 |
spellingShingle | Sefti Heza Dwinanti , Tanbiyaskur Modification of non-selective-solid media for aquatic bacteria Jurnal Akuakultur Indonesia |
title | Modification of non-selective-solid media for aquatic bacteria |
title_full | Modification of non-selective-solid media for aquatic bacteria |
title_fullStr | Modification of non-selective-solid media for aquatic bacteria |
title_full_unstemmed | Modification of non-selective-solid media for aquatic bacteria |
title_short | Modification of non-selective-solid media for aquatic bacteria |
title_sort | modification of non selective solid media for aquatic bacteria |
url | https://journal.ipb.ac.id/index.php/jai/article/view/10317 |
work_keys_str_mv | AT seftihezadwinanti modificationofnonselectivesolidmediaforaquaticbacteria AT tanbiyaskur modificationofnonselectivesolidmediaforaquaticbacteria |