Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry for Identification of In Vitro and In Vivo Metabolites of Bornyl Gallate in Rats
Bornyl gallate (BG) is a potential drug candidate synthesized by the reaction of two natural products, gallic acid and borneol. Previous studies have strongly suggested that BG is worthy of further investigation due to antioxidant, antiatherosclerosis activities, and obvious activity of stimulating...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/473649 |
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| author | Wei Lan Liujiao Bian Xinfeng Zhao Pu Jia Xue Meng Yizhen Wu Shixiang Wang Sha Liao Jie Yu Xiaohui Zheng |
| author_facet | Wei Lan Liujiao Bian Xinfeng Zhao Pu Jia Xue Meng Yizhen Wu Shixiang Wang Sha Liao Jie Yu Xiaohui Zheng |
| author_sort | Wei Lan |
| collection | DOAJ |
| description | Bornyl gallate (BG) is a potential drug candidate synthesized by the reaction of two natural products, gallic acid and borneol. Previous studies have strongly suggested that BG is worthy of further investigation due to antioxidant, antiatherosclerosis activities, and obvious activity of stimulating intersegmental vessel growth in zebrafish. This work was designed to elucidate the metabolic profile of BG through analyzing its metabolites in vitro and in vivo by a chromatographic separation coupled with a mass spectrometry. The metabolites of BG were characterized from the rat liver microsome incubation solution, as well as rat urine and plasma after oral administration. Chromatographic separation was performed on an Agilent TC-C18 column (250 mm × 4.6 mm, 5 μm) with gradient elution using methanol and water containing 0.2% (V : V) formic acid as the mobile phase. Metabolites identification involved analyzing the retention behaviors, changes of molecular weights and MS/MS fragment patterns of BG and the metabolites. Five compounds were identified as isomers of hydroxylated BG metabolites in vitro. The major metabolites of BG in rat urine and plasma proved to be BG-O-glucuronide and O-methyl BG-O-glucuronide. The proposed method confirmed to be a reliable and sensitive alternative for characterizing metabolic pathways of BG. |
| format | Article |
| id | doaj-art-169d34610b154b438b05d72a8cadf534 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-169d34610b154b438b05d72a8cadf5342025-02-03T06:00:10ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732013-01-01201310.1155/2013/473649473649Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry for Identification of In Vitro and In Vivo Metabolites of Bornyl Gallate in RatsWei Lan0Liujiao Bian1Xinfeng Zhao2Pu Jia3Xue Meng4Yizhen Wu5Shixiang Wang6Sha Liao7Jie Yu8Xiaohui Zheng9College of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, ChinaCollege of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, ChinaCollege of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, ChinaCollege of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, ChinaCollege of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, ChinaCollege of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, ChinaCollege of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, ChinaCollege of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, ChinaCollege of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, ChinaCollege of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, ChinaBornyl gallate (BG) is a potential drug candidate synthesized by the reaction of two natural products, gallic acid and borneol. Previous studies have strongly suggested that BG is worthy of further investigation due to antioxidant, antiatherosclerosis activities, and obvious activity of stimulating intersegmental vessel growth in zebrafish. This work was designed to elucidate the metabolic profile of BG through analyzing its metabolites in vitro and in vivo by a chromatographic separation coupled with a mass spectrometry. The metabolites of BG were characterized from the rat liver microsome incubation solution, as well as rat urine and plasma after oral administration. Chromatographic separation was performed on an Agilent TC-C18 column (250 mm × 4.6 mm, 5 μm) with gradient elution using methanol and water containing 0.2% (V : V) formic acid as the mobile phase. Metabolites identification involved analyzing the retention behaviors, changes of molecular weights and MS/MS fragment patterns of BG and the metabolites. Five compounds were identified as isomers of hydroxylated BG metabolites in vitro. The major metabolites of BG in rat urine and plasma proved to be BG-O-glucuronide and O-methyl BG-O-glucuronide. The proposed method confirmed to be a reliable and sensitive alternative for characterizing metabolic pathways of BG.http://dx.doi.org/10.1155/2013/473649 |
| spellingShingle | Wei Lan Liujiao Bian Xinfeng Zhao Pu Jia Xue Meng Yizhen Wu Shixiang Wang Sha Liao Jie Yu Xiaohui Zheng Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry for Identification of In Vitro and In Vivo Metabolites of Bornyl Gallate in Rats Journal of Analytical Methods in Chemistry |
| title | Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry for Identification of In Vitro and In Vivo Metabolites of Bornyl Gallate in Rats |
| title_full | Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry for Identification of In Vitro and In Vivo Metabolites of Bornyl Gallate in Rats |
| title_fullStr | Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry for Identification of In Vitro and In Vivo Metabolites of Bornyl Gallate in Rats |
| title_full_unstemmed | Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry for Identification of In Vitro and In Vivo Metabolites of Bornyl Gallate in Rats |
| title_short | Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry for Identification of In Vitro and In Vivo Metabolites of Bornyl Gallate in Rats |
| title_sort | liquid chromatography quadrupole time of flight mass spectrometry for identification of in vitro and in vivo metabolites of bornyl gallate in rats |
| url | http://dx.doi.org/10.1155/2013/473649 |
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