In Vivo Chemoprotective Activity of Bovine Dialyzable Leukocyte Extract in Mouse Bone Marrow Cells against Damage Induced by 5-Fluorouracil
Chemotherapy treatments induce a number of side effects, such as leukopenia neutropenia, peripheral erythropenia, and thrombocytopenia, affecting the quality of life for cancer patients. 5-Fluorouracil (5-FU) is wieldy used as myeloablative model in mice. The bovine dialyzable leukocyte extract (bDL...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/6942321 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832562033472569344 |
|---|---|
| author | Erika Evangelina Coronado-Cerda Moisés Armides Franco-Molina Edgar Mendoza-Gamboa Heriberto Prado-García Lydia Guadalupe Rivera-Morales Pablo Zapata-Benavides María del Carmen Rodríguez-Salazar Diana Caballero-Hernandez Reyes Silvestre Tamez-Guerra Cristina Rodríguez-Padilla |
| author_facet | Erika Evangelina Coronado-Cerda Moisés Armides Franco-Molina Edgar Mendoza-Gamboa Heriberto Prado-García Lydia Guadalupe Rivera-Morales Pablo Zapata-Benavides María del Carmen Rodríguez-Salazar Diana Caballero-Hernandez Reyes Silvestre Tamez-Guerra Cristina Rodríguez-Padilla |
| author_sort | Erika Evangelina Coronado-Cerda |
| collection | DOAJ |
| description | Chemotherapy treatments induce a number of side effects, such as leukopenia neutropenia, peripheral erythropenia, and thrombocytopenia, affecting the quality of life for cancer patients. 5-Fluorouracil (5-FU) is wieldy used as myeloablative model in mice. The bovine dialyzable leukocyte extract (bDLE) or IMMUNEPOTENT CRP® (ICRP) is an immunomodulatory compound that has antioxidants and anti-inflammatory effects. In order to investigate the chemoprotection effect of ICRP on bone marrow cells in 5-FU treated mice, total bone marrow (BM) cell count, bone marrow colony forming units-granulocyte/macrophage (CFU-GM), cell cycle, immunophenotypification, ROS/superoxide and Nrf2 by flow cytometry, and histological and hematological analyses were performed. Our results demonstrated that ICRP increased BM cell count and CFU-GM number, arrested BM cells in G0/G1 phase, increased the percentage of leukocyte, granulocytic, and erythroid populations, reduced ROS/superoxide formation and Nrf2 activation, and also improved hematological levels and weight gain in 5-FU treated mice. These results suggest that ICRP has a chemoprotective effect against 5-FU in BM cells that can be used in cancer patients. |
| format | Article |
| id | doaj-art-1698f89173cf416e873c780aa099b18f |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-1698f89173cf416e873c780aa099b18f2025-02-03T01:23:31ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/69423216942321In Vivo Chemoprotective Activity of Bovine Dialyzable Leukocyte Extract in Mouse Bone Marrow Cells against Damage Induced by 5-FluorouracilErika Evangelina Coronado-Cerda0Moisés Armides Franco-Molina1Edgar Mendoza-Gamboa2Heriberto Prado-García3Lydia Guadalupe Rivera-Morales4Pablo Zapata-Benavides5María del Carmen Rodríguez-Salazar6Diana Caballero-Hernandez7Reyes Silvestre Tamez-Guerra8Cristina Rodríguez-Padilla9Laboratorio de Inmunologia y Virologia, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), P.O. Box 46 “F”, 66455 San Nicolás de los Garza, NL, MexicoLaboratorio de Inmunologia y Virologia, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), P.O. Box 46 “F”, 66455 San Nicolás de los Garza, NL, MexicoLaboratorio de Inmunologia y Virologia, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), P.O. Box 46 “F”, 66455 San Nicolás de los Garza, NL, MexicoDepartamento de Enfermedades Crónico-Degenerativas, Instituto Nacional de Enfermedades Respiratorias, Tlalpan 4502, Colonia Sección XVI, 14080 Ciudad de México, DF, MexicoLaboratorio de Inmunologia y Virologia, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), P.O. Box 46 “F”, 66455 San Nicolás de los Garza, NL, MexicoLaboratorio de Inmunologia y Virologia, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), P.O. Box 46 “F”, 66455 San Nicolás de los Garza, NL, MexicoLaboratorio de Inmunologia y Virologia, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), P.O. Box 46 “F”, 66455 San Nicolás de los Garza, NL, MexicoLaboratorio de Inmunologia y Virologia, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), P.O. Box 46 “F”, 66455 San Nicolás de los Garza, NL, MexicoLaboratorio de Inmunologia y Virologia, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), P.O. Box 46 “F”, 66455 San Nicolás de los Garza, NL, MexicoLaboratorio de Inmunologia y Virologia, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), P.O. Box 46 “F”, 66455 San Nicolás de los Garza, NL, MexicoChemotherapy treatments induce a number of side effects, such as leukopenia neutropenia, peripheral erythropenia, and thrombocytopenia, affecting the quality of life for cancer patients. 5-Fluorouracil (5-FU) is wieldy used as myeloablative model in mice. The bovine dialyzable leukocyte extract (bDLE) or IMMUNEPOTENT CRP® (ICRP) is an immunomodulatory compound that has antioxidants and anti-inflammatory effects. In order to investigate the chemoprotection effect of ICRP on bone marrow cells in 5-FU treated mice, total bone marrow (BM) cell count, bone marrow colony forming units-granulocyte/macrophage (CFU-GM), cell cycle, immunophenotypification, ROS/superoxide and Nrf2 by flow cytometry, and histological and hematological analyses were performed. Our results demonstrated that ICRP increased BM cell count and CFU-GM number, arrested BM cells in G0/G1 phase, increased the percentage of leukocyte, granulocytic, and erythroid populations, reduced ROS/superoxide formation and Nrf2 activation, and also improved hematological levels and weight gain in 5-FU treated mice. These results suggest that ICRP has a chemoprotective effect against 5-FU in BM cells that can be used in cancer patients.http://dx.doi.org/10.1155/2016/6942321 |
| spellingShingle | Erika Evangelina Coronado-Cerda Moisés Armides Franco-Molina Edgar Mendoza-Gamboa Heriberto Prado-García Lydia Guadalupe Rivera-Morales Pablo Zapata-Benavides María del Carmen Rodríguez-Salazar Diana Caballero-Hernandez Reyes Silvestre Tamez-Guerra Cristina Rodríguez-Padilla In Vivo Chemoprotective Activity of Bovine Dialyzable Leukocyte Extract in Mouse Bone Marrow Cells against Damage Induced by 5-Fluorouracil Journal of Immunology Research |
| title | In Vivo Chemoprotective Activity of Bovine Dialyzable Leukocyte Extract in Mouse Bone Marrow Cells against Damage Induced by 5-Fluorouracil |
| title_full | In Vivo Chemoprotective Activity of Bovine Dialyzable Leukocyte Extract in Mouse Bone Marrow Cells against Damage Induced by 5-Fluorouracil |
| title_fullStr | In Vivo Chemoprotective Activity of Bovine Dialyzable Leukocyte Extract in Mouse Bone Marrow Cells against Damage Induced by 5-Fluorouracil |
| title_full_unstemmed | In Vivo Chemoprotective Activity of Bovine Dialyzable Leukocyte Extract in Mouse Bone Marrow Cells against Damage Induced by 5-Fluorouracil |
| title_short | In Vivo Chemoprotective Activity of Bovine Dialyzable Leukocyte Extract in Mouse Bone Marrow Cells against Damage Induced by 5-Fluorouracil |
| title_sort | in vivo chemoprotective activity of bovine dialyzable leukocyte extract in mouse bone marrow cells against damage induced by 5 fluorouracil |
| url | http://dx.doi.org/10.1155/2016/6942321 |
| work_keys_str_mv | AT erikaevangelinacoronadocerda invivochemoprotectiveactivityofbovinedialyzableleukocyteextractinmousebonemarrowcellsagainstdamageinducedby5fluorouracil AT moisesarmidesfrancomolina invivochemoprotectiveactivityofbovinedialyzableleukocyteextractinmousebonemarrowcellsagainstdamageinducedby5fluorouracil AT edgarmendozagamboa invivochemoprotectiveactivityofbovinedialyzableleukocyteextractinmousebonemarrowcellsagainstdamageinducedby5fluorouracil AT heribertopradogarcia invivochemoprotectiveactivityofbovinedialyzableleukocyteextractinmousebonemarrowcellsagainstdamageinducedby5fluorouracil AT lydiaguadaluperiveramorales invivochemoprotectiveactivityofbovinedialyzableleukocyteextractinmousebonemarrowcellsagainstdamageinducedby5fluorouracil AT pablozapatabenavides invivochemoprotectiveactivityofbovinedialyzableleukocyteextractinmousebonemarrowcellsagainstdamageinducedby5fluorouracil AT mariadelcarmenrodriguezsalazar invivochemoprotectiveactivityofbovinedialyzableleukocyteextractinmousebonemarrowcellsagainstdamageinducedby5fluorouracil AT dianacaballerohernandez invivochemoprotectiveactivityofbovinedialyzableleukocyteextractinmousebonemarrowcellsagainstdamageinducedby5fluorouracil AT reyessilvestretamezguerra invivochemoprotectiveactivityofbovinedialyzableleukocyteextractinmousebonemarrowcellsagainstdamageinducedby5fluorouracil AT cristinarodriguezpadilla invivochemoprotectiveactivityofbovinedialyzableleukocyteextractinmousebonemarrowcellsagainstdamageinducedby5fluorouracil |