Radical Reactions in the Gas Phase: Recent Development and Application in Biomolecules
This review summarizes recent literature describing the use of gas phase radical reactions for structural characterization of complex biomolecules other than peptides. Specifically, chemical derivatization, in-source chemical reaction, and gas phase ion/ion reactions have been demonstrated as effect...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Spectroscopy |
| Online Access: | http://dx.doi.org/10.1155/2014/570863 |
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| _version_ | 1832549075771195392 |
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| author | Yang Gao Jiexun Bu Zhou Peng Biwei Yang |
| author_facet | Yang Gao Jiexun Bu Zhou Peng Biwei Yang |
| author_sort | Yang Gao |
| collection | DOAJ |
| description | This review summarizes recent literature describing the use of gas phase radical reactions for structural characterization of complex biomolecules other than peptides. Specifically, chemical derivatization, in-source chemical reaction, and gas phase ion/ion reactions have been demonstrated as effective ways to generate radical precursor ions that yield structural informative fragments complementary to those from conventional collision-induced dissociation (CID). Radical driven dissociation has been applied to a variety of biomolecules including peptides, nucleic acids, carbohydrates, and phospholipids. The majority of the molecules discussed in this review see limited fragmentation from conventional CID, and the gas phase radical reactions open up completely new dissociation channels for these molecules and therefore yield high fidelity confirmation of the structures of the target molecules. Due to the extensively studied peptide fragmentation, this review focuses only on nonpeptide biomolecules such as nucleic acids, carbohydrates, and phospholipids. |
| format | Article |
| id | doaj-art-166b3f82bc994b8dbb5f967e6813219c |
| institution | Kabale University |
| issn | 2314-4920 2314-4939 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Spectroscopy |
| spelling | doaj-art-166b3f82bc994b8dbb5f967e6813219c2025-02-03T06:12:17ZengWileyJournal of Spectroscopy2314-49202314-49392014-01-01201410.1155/2014/570863570863Radical Reactions in the Gas Phase: Recent Development and Application in BiomoleculesYang Gao0Jiexun Bu1Zhou Peng2Biwei Yang3Bioanalytical Chemistry, Covance Laboratories, Inc., 3301 Kinsman Boulevard, Madison, WI 53704, USADepartment of Chemistry, Purdue University, West Lafayette, IN 47907, USADepartment of Chemistry, Purdue University, West Lafayette, IN 47907, USADepartment of Chemistry, Purdue University, West Lafayette, IN 47907, USAThis review summarizes recent literature describing the use of gas phase radical reactions for structural characterization of complex biomolecules other than peptides. Specifically, chemical derivatization, in-source chemical reaction, and gas phase ion/ion reactions have been demonstrated as effective ways to generate radical precursor ions that yield structural informative fragments complementary to those from conventional collision-induced dissociation (CID). Radical driven dissociation has been applied to a variety of biomolecules including peptides, nucleic acids, carbohydrates, and phospholipids. The majority of the molecules discussed in this review see limited fragmentation from conventional CID, and the gas phase radical reactions open up completely new dissociation channels for these molecules and therefore yield high fidelity confirmation of the structures of the target molecules. Due to the extensively studied peptide fragmentation, this review focuses only on nonpeptide biomolecules such as nucleic acids, carbohydrates, and phospholipids.http://dx.doi.org/10.1155/2014/570863 |
| spellingShingle | Yang Gao Jiexun Bu Zhou Peng Biwei Yang Radical Reactions in the Gas Phase: Recent Development and Application in Biomolecules Journal of Spectroscopy |
| title | Radical Reactions in the Gas Phase: Recent Development and Application in Biomolecules |
| title_full | Radical Reactions in the Gas Phase: Recent Development and Application in Biomolecules |
| title_fullStr | Radical Reactions in the Gas Phase: Recent Development and Application in Biomolecules |
| title_full_unstemmed | Radical Reactions in the Gas Phase: Recent Development and Application in Biomolecules |
| title_short | Radical Reactions in the Gas Phase: Recent Development and Application in Biomolecules |
| title_sort | radical reactions in the gas phase recent development and application in biomolecules |
| url | http://dx.doi.org/10.1155/2014/570863 |
| work_keys_str_mv | AT yanggao radicalreactionsinthegasphaserecentdevelopmentandapplicationinbiomolecules AT jiexunbu radicalreactionsinthegasphaserecentdevelopmentandapplicationinbiomolecules AT zhoupeng radicalreactionsinthegasphaserecentdevelopmentandapplicationinbiomolecules AT biweiyang radicalreactionsinthegasphaserecentdevelopmentandapplicationinbiomolecules |